Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22180 and BPHYT_RS22185 are separated by 56 nucleotides BPHYT_RS22185 and BPHYT_RS22190 are separated by 603 nucleotides BPHYT_RS22190 and BPHYT_RS22195 are separated by 22 nucleotides BPHYT_RS22195 and BPHYT_RS22200 are separated by 146 nucleotides
BPHYT_RS22180: BPHYT_RS22180 - hypothetical protein, at 536,031 to 536,270
_RS22180
BPHYT_RS22185: BPHYT_RS22185 - hypothetical protein, at 536,327 to 536,617
_RS22185
BPHYT_RS22190: BPHYT_RS22190 - nucleoid-structuring protein H-NS, at 537,221 to 538,072
_RS22190
BPHYT_RS22195: BPHYT_RS22195 - hypothetical protein, at 538,095 to 538,580
_RS22195
BPHYT_RS22200: BPHYT_RS22200 - cardiolipin synthetase, at 538,727 to 539,989
_RS22200
Position (kb) 537
538
539 Strain fitness (log2 ratio) -1
0
1
2
3 at 536.257 kb on + strand at 536.258 kb on - strand at 536.258 kb on - strand at 536.313 kb on + strand at 536.316 kb on - strand at 536.487 kb on + strand, within BPHYT_RS22185 at 536.718 kb on - strand at 536.853 kb on + strand at 536.920 kb on + strand at 536.922 kb on + strand at 536.936 kb on + strand at 537.452 kb on + strand, within BPHYT_RS22190 at 537.916 kb on - strand, within BPHYT_RS22190 at 538.093 kb on - strand at 538.310 kb on - strand, within BPHYT_RS22195 at 538.414 kb on + strand, within BPHYT_RS22195 at 538.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB remove 536,257 + -1.4 536,258 - +0.1 536,258 - +0.1 536,313 + +2.8 536,316 - +0.4 536,487 + BPHYT_RS22185 0.55 +0.7 536,718 - +0.8 536,853 + -0.9 536,920 + +1.0 536,922 + +1.0 536,936 + +1.3 537,452 + BPHYT_RS22190 0.27 -0.9 537,916 - BPHYT_RS22190 0.82 -1.6 538,093 - +1.2 538,310 - BPHYT_RS22195 0.44 -0.4 538,414 + BPHYT_RS22195 0.66 +1.0 538,833 - -0.1
Or see this region's nucleotide sequence