Experiment: Betaine (C)(N); with TAPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS04065 and PFLU_RS04070 are separated by 331 nucleotides PFLU_RS04070 and PFLU_RS04075 are separated by 259 nucleotides PFLU_RS04075 and PFLU_RS04080 are separated by 98 nucleotides
PFLU_RS04065: PFLU_RS04065 - ABC transporter permease, at 921,348 to 922,064
_RS04065
PFLU_RS04070: PFLU_RS04070 - peptide chain release factor 3, at 922,396 to 923,979
_RS04070
PFLU_RS04075: PFLU_RS04075 - hypothetical protein, at 924,239 to 924,484
_RS04075
PFLU_RS04080: PFLU_RS04080 - hypothetical protein, at 924,583 to 924,846
_RS04080
Position (kb) 922
923
924 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 921.615 kb on + strand, within PFLU_RS04065 at 921.616 kb on - strand, within PFLU_RS04065 at 921.616 kb on - strand, within PFLU_RS04065 at 921.616 kb on - strand, within PFLU_RS04065 at 921.687 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.688 kb on + strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.689 kb on - strand, within PFLU_RS04065 at 921.704 kb on + strand, within PFLU_RS04065 at 921.705 kb on - strand, within PFLU_RS04065 at 921.807 kb on - strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.915 kb on + strand, within PFLU_RS04065 at 921.916 kb on - strand, within PFLU_RS04065 at 921.916 kb on - strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.943 kb on + strand, within PFLU_RS04065 at 921.965 kb on - strand, within PFLU_RS04065 at 922.035 kb on + strand at 922.035 kb on + strand at 922.036 kb on - strand at 922.036 kb on - strand at 922.213 kb on - strand at 922.226 kb on - strand at 922.250 kb on - strand at 922.270 kb on + strand at 922.271 kb on - strand at 922.271 kb on - strand at 922.271 kb on - strand at 922.271 kb on - strand at 922.303 kb on + strand at 922.304 kb on - strand at 922.304 kb on - strand at 922.354 kb on + strand at 922.355 kb on - strand at 922.355 kb on - strand at 922.355 kb on - strand at 922.443 kb on + strand at 922.443 kb on + strand at 922.444 kb on - strand at 922.590 kb on + strand, within PFLU_RS04070 at 922.599 kb on + strand, within PFLU_RS04070 at 922.600 kb on - strand, within PFLU_RS04070 at 922.687 kb on + strand, within PFLU_RS04070 at 922.688 kb on - strand, within PFLU_RS04070 at 922.936 kb on + strand, within PFLU_RS04070 at 922.936 kb on + strand, within PFLU_RS04070 at 922.936 kb on + strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.937 kb on - strand, within PFLU_RS04070 at 922.954 kb on + strand, within PFLU_RS04070 at 922.966 kb on + strand, within PFLU_RS04070 at 922.967 kb on - strand, within PFLU_RS04070 at 922.967 kb on - strand, within PFLU_RS04070 at 923.360 kb on - strand, within PFLU_RS04070 at 923.360 kb on - strand, within PFLU_RS04070 at 923.755 kb on + strand, within PFLU_RS04070 at 923.756 kb on - strand, within PFLU_RS04070 at 923.756 kb on - strand, within PFLU_RS04070 at 923.756 kb on - strand, within PFLU_RS04070 at 923.756 kb on - strand, within PFLU_RS04070 at 923.773 kb on + strand, within PFLU_RS04070 at 923.773 kb on + strand, within PFLU_RS04070 at 923.774 kb on - strand, within PFLU_RS04070 at 923.774 kb on - strand, within PFLU_RS04070 at 923.774 kb on - strand, within PFLU_RS04070 at 923.791 kb on + strand, within PFLU_RS04070 at 923.893 kb on + strand at 923.894 kb on - strand at 923.977 kb on + strand at 923.977 kb on + strand at 923.978 kb on - strand at 924.076 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.084 kb on + strand at 924.094 kb on + strand at 924.094 kb on + strand at 924.094 kb on + strand at 924.111 kb on - strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.180 kb on + strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.181 kb on - strand at 924.191 kb on + strand at 924.191 kb on + strand at 924.203 kb on + strand at 924.204 kb on - strand at 924.204 kb on - strand at 924.212 kb on + strand at 924.212 kb on + strand at 924.213 kb on - strand at 924.213 kb on - strand at 924.227 kb on + strand at 924.227 kb on + strand at 924.227 kb on + strand at 924.228 kb on - strand at 924.228 kb on - strand at 924.228 kb on - strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.244 kb on + strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.245 kb on - strand at 924.246 kb on + strand at 924.246 kb on + strand at 924.246 kb on + strand at 924.246 kb on + strand at 924.247 kb on - strand at 924.247 kb on - strand at 924.247 kb on - strand at 924.247 kb on - strand at 924.247 kb on - strand at 924.438 kb on - strand, within PFLU_RS04075 at 924.438 kb on - strand, within PFLU_RS04075 at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.464 kb on + strand at 924.465 kb on - strand at 924.465 kb on - strand at 924.465 kb on - strand at 924.465 kb on - strand at 924.465 kb on - strand at 924.465 kb on - strand at 924.485 kb on + strand at 924.485 kb on + strand at 924.486 kb on - strand at 924.486 kb on - strand at 924.486 kb on - strand at 924.503 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.512 kb on + strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.513 kb on - strand at 924.560 kb on + strand at 924.560 kb on + strand at 924.560 kb on + strand at 924.561 kb on - strand at 924.561 kb on - strand at 924.561 kb on - strand at 924.561 kb on - strand at 924.588 kb on + strand at 924.589 kb on - strand at 924.589 kb on - strand at 924.614 kb on + strand, within PFLU_RS04080 at 924.614 kb on + strand, within PFLU_RS04080 at 924.614 kb on + strand, within PFLU_RS04080 at 924.615 kb on - strand, within PFLU_RS04080 at 924.615 kb on - strand, within PFLU_RS04080 at 924.642 kb on + strand, within PFLU_RS04080 at 924.642 kb on + strand, within PFLU_RS04080 at 924.642 kb on + strand, within PFLU_RS04080 at 924.642 kb on + strand, within PFLU_RS04080 at 924.643 kb on - strand, within PFLU_RS04080 at 924.643 kb on - strand, within PFLU_RS04080 at 924.643 kb on - strand, within PFLU_RS04080 at 924.643 kb on - strand, within PFLU_RS04080 at 924.663 kb on + strand, within PFLU_RS04080 at 924.663 kb on + strand, within PFLU_RS04080 at 924.663 kb on + strand, within PFLU_RS04080 at 924.663 kb on + strand, within PFLU_RS04080 at 924.664 kb on - strand, within PFLU_RS04080 at 924.664 kb on - strand, within PFLU_RS04080 at 924.664 kb on - strand, within PFLU_RS04080 at 924.844 kb on + strand at 924.844 kb on + strand at 924.844 kb on + strand at 924.845 kb on - strand at 924.845 kb on - strand at 924.845 kb on - strand at 924.845 kb on - strand at 924.845 kb on - strand at 924.927 kb on + strand at 924.928 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Betaine (C)(N); with TAPS; with chloride remove 921,615 + PFLU_RS04065 0.37 -1.7 921,616 - PFLU_RS04065 0.37 -0.4 921,616 - PFLU_RS04065 0.37 -0.5 921,616 - PFLU_RS04065 0.37 -1.3 921,687 + PFLU_RS04065 0.47 -2.0 921,688 + PFLU_RS04065 0.47 -1.5 921,688 + PFLU_RS04065 0.47 -2.6 921,688 + PFLU_RS04065 0.47 -2.3 921,688 + PFLU_RS04065 0.47 -1.9 921,688 + PFLU_RS04065 0.47 -1.2 921,688 + PFLU_RS04065 0.47 -3.4 921,688 + PFLU_RS04065 0.47 -2.3 921,688 + PFLU_RS04065 0.47 -2.7 921,688 + PFLU_RS04065 0.47 -0.9 921,688 + PFLU_RS04065 0.47 -2.8 921,688 + PFLU_RS04065 0.47 -0.1 921,688 + PFLU_RS04065 0.47 -0.1 921,688 + PFLU_RS04065 0.47 -0.9 921,688 + PFLU_RS04065 0.47 -1.1 921,688 + PFLU_RS04065 0.47 -3.1 921,689 - PFLU_RS04065 0.48 +1.1 921,689 - PFLU_RS04065 0.48 -1.5 921,689 - PFLU_RS04065 0.48 -2.1 921,689 - PFLU_RS04065 0.48 -3.0 921,689 - PFLU_RS04065 0.48 -2.8 921,689 - PFLU_RS04065 0.48 -2.2 921,689 - PFLU_RS04065 0.48 -1.2 921,689 - PFLU_RS04065 0.48 -1.6 921,704 + PFLU_RS04065 0.50 -2.1 921,705 - PFLU_RS04065 0.50 -2.1 921,807 - PFLU_RS04065 0.64 -2.8 921,915 + PFLU_RS04065 0.79 -1.3 921,915 + PFLU_RS04065 0.79 -2.8 921,915 + PFLU_RS04065 0.79 -2.7 921,915 + PFLU_RS04065 0.79 -2.4 921,915 + PFLU_RS04065 0.79 +1.8 921,916 - PFLU_RS04065 0.79 +0.7 921,916 - PFLU_RS04065 0.79 -3.1 921,943 + PFLU_RS04065 0.83 +0.3 921,943 + PFLU_RS04065 0.83 -0.5 921,943 + PFLU_RS04065 0.83 -1.2 921,943 + PFLU_RS04065 0.83 +0.6 921,965 - PFLU_RS04065 0.86 -1.8 922,035 + -1.8 922,035 + -1.3 922,036 - -3.2 922,036 - -1.1 922,213 - -0.9 922,226 - +1.8 922,250 - +0.9 922,270 + -0.9 922,271 - +1.2 922,271 - +2.6 922,271 - +1.9 922,271 - +2.6 922,303 + -0.8 922,304 - +3.4 922,304 - +1.6 922,354 + -0.3 922,355 - +3.8 922,355 - +0.8 922,355 - -0.1 922,443 + +1.3 922,443 + -0.1 922,444 - +1.3 922,590 + PFLU_RS04070 0.12 +2.3 922,599 + PFLU_RS04070 0.13 +3.9 922,600 - PFLU_RS04070 0.13 +2.9 922,687 + PFLU_RS04070 0.18 -0.1 922,688 - PFLU_RS04070 0.18 +3.9 922,936 + PFLU_RS04070 0.34 -0.1 922,936 + PFLU_RS04070 0.34 +1.3 922,936 + PFLU_RS04070 0.34 +0.7 922,937 - PFLU_RS04070 0.34 +0.7 922,937 - PFLU_RS04070 0.34 +1.3 922,937 - PFLU_RS04070 0.34 +1.3 922,937 - PFLU_RS04070 0.34 +0.7 922,954 + PFLU_RS04070 0.35 +1.3 922,966 + PFLU_RS04070 0.36 +2.3 922,967 - PFLU_RS04070 0.36 +2.3 922,967 - PFLU_RS04070 0.36 +2.3 923,360 - PFLU_RS04070 0.61 +1.3 923,360 - PFLU_RS04070 0.61 -0.7 923,755 + PFLU_RS04070 0.86 +2.3 923,756 - PFLU_RS04070 0.86 +4.6 923,756 - PFLU_RS04070 0.86 +2.3 923,756 - PFLU_RS04070 0.86 +1.3 923,756 - PFLU_RS04070 0.86 +2.3 923,773 + PFLU_RS04070 0.87 -0.1 923,773 + PFLU_RS04070 0.87 +0.7 923,774 - PFLU_RS04070 0.87 +1.3 923,774 - PFLU_RS04070 0.87 +1.3 923,774 - PFLU_RS04070 0.87 -0.5 923,791 + PFLU_RS04070 0.88 -0.5 923,893 + -0.5 923,894 - +0.7 923,977 + +0.3 923,977 + +1.1 923,978 - -0.1 924,076 + -0.7 924,084 + -0.3 924,084 + -0.9 924,084 + -0.9 924,084 + +0.5 924,084 + +0.3 924,084 + +1.7 924,084 + +0.3 924,084 + +1.4 924,084 + +1.9 924,084 + -1.5 924,094 + +1.9 924,094 + +0.9 924,094 + +2.6 924,111 - +1.4 924,180 + +2.1 924,180 + -0.9 924,180 + +0.9 924,180 + +1.1 924,180 + +2.3 924,180 + +0.7 924,180 + -0.9 924,180 + -0.5 924,180 + +1.0 924,180 + -1.8 924,180 + -2.5 924,180 + +0.7 924,180 + -0.5 924,180 + -0.1 924,180 + +1.3 924,180 + +0.5 924,180 + -1.2 924,180 + -2.7 924,181 - -1.1 924,181 - -1.6 924,181 - +0.4 924,181 - +0.8 924,181 - -1.4 924,181 - +0.3 924,181 - -0.3 924,181 - +1.6 924,181 - -0.1 924,181 - +0.3 924,181 - -0.7 924,181 - +0.5 924,181 - -2.2 924,181 - +1.6 924,181 - +0.3 924,181 - -0.6 924,181 - +0.2 924,181 - -0.7 924,191 + -0.5 924,191 + +1.2 924,203 + +1.1 924,204 - +1.4 924,204 - +0.7 924,212 + -2.1 924,212 + +0.9 924,213 - +0.3 924,213 - +0.6 924,227 + +1.0 924,227 + +3.2 924,227 + -0.1 924,228 - +1.5 924,228 - -2.5 924,228 - -1.1 924,244 + +1.7 924,244 + +1.7 924,244 + +0.7 924,244 + -2.3 924,244 + +2.7 924,244 + -0.2 924,244 + -1.3 924,244 + +0.5 924,244 + -0.1 924,244 + +0.9 924,244 + +0.4 924,244 + -0.9 924,245 - -1.8 924,245 - -0.9 924,245 - +1.3 924,245 - -1.6 924,245 - +0.2 924,245 - +0.3 924,245 - -0.3 924,245 - +0.2 924,245 - +0.8 924,245 - +1.6 924,245 - -0.7 924,245 - +1.5 924,245 - -2.6 924,245 - -0.1 924,245 - +0.2 924,245 - +0.9 924,246 + +1.9 924,246 + +0.7 924,246 + -1.1 924,246 + -0.2 924,247 - +0.9 924,247 - -2.4 924,247 - -1.3 924,247 - -0.1 924,247 - -1.1 924,438 - PFLU_RS04075 0.81 +0.3 924,438 - PFLU_RS04075 0.81 -1.8 924,464 + +0.3 924,464 + -0.7 924,464 + +1.2 924,464 + -0.3 924,464 + +0.3 924,464 + +1.6 924,464 + -0.1 924,464 + -2.1 924,464 + +2.3 924,464 + -1.0 924,464 + -0.6 924,464 + +2.2 924,464 + -2.3 924,464 + -0.5 924,465 - -1.2 924,465 - -0.9 924,465 - -0.3 924,465 - +0.4 924,465 - -0.4 924,465 - +1.6 924,485 + -0.2 924,485 + -0.2 924,486 - +1.4 924,486 - +0.9 924,486 - -0.1 924,503 + +0.7 924,512 + +1.0 924,512 + -1.1 924,512 + +2.9 924,512 + -1.3 924,512 + +1.9 924,512 + -1.3 924,512 + -0.5 924,512 + +2.4 924,512 + +0.6 924,513 - -0.8 924,513 - +1.9 924,513 - +1.1 924,513 - -1.8 924,513 - -0.5 924,513 - -2.3 924,513 - +2.1 924,513 - -0.5 924,513 - +0.6 924,513 - +0.6 924,560 + -0.6 924,560 + +2.6 924,560 + -0.7 924,561 - +1.7 924,561 - -0.5 924,561 - -0.8 924,561 - -0.6 924,588 + +0.9 924,589 - +0.3 924,589 - -0.1 924,614 + PFLU_RS04080 0.12 +2.1 924,614 + PFLU_RS04080 0.12 +1.1 924,614 + PFLU_RS04080 0.12 -0.6 924,615 - PFLU_RS04080 0.12 +0.2 924,615 - PFLU_RS04080 0.12 -1.9 924,642 + PFLU_RS04080 0.22 -1.1 924,642 + PFLU_RS04080 0.22 +1.3 924,642 + PFLU_RS04080 0.22 +0.8 924,642 + PFLU_RS04080 0.22 -2.1 924,643 - PFLU_RS04080 0.23 -1.1 924,643 - PFLU_RS04080 0.23 +0.3 924,643 - PFLU_RS04080 0.23 -0.6 924,643 - PFLU_RS04080 0.23 -1.5 924,663 + PFLU_RS04080 0.30 +0.3 924,663 + PFLU_RS04080 0.30 +2.4 924,663 + PFLU_RS04080 0.30 +1.5 924,663 + PFLU_RS04080 0.30 +1.7 924,664 - PFLU_RS04080 0.31 +1.2 924,664 - PFLU_RS04080 0.31 +0.3 924,664 - PFLU_RS04080 0.31 -1.5 924,844 + +0.8 924,844 + -2.9 924,844 + -1.9 924,845 - -0.1 924,845 - -0.1 924,845 - -0.5 924,845 - -1.6 924,845 - +1.2 924,927 + +1.2 924,928 - +0.5
Or see this region's nucleotide sequence