Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4484 and BT4485 are separated by 208 nucleotides BT4485 and BT4486 are separated by 33 nucleotides BT4486 and BT4487 are separated by 83 nucleotides
BT4484: BT4484 - conserved hypothetical protein (NCBI ptt file), at 5,901,239 to 5,901,847
BT4484
BT4485: BT4485 - conserved hypothetical protein (NCBI ptt file), at 5,902,056 to 5,902,421
BT4485
BT4486: BT4486 - voltage-gated K+ channel protein (NCBI ptt file), at 5,902,455 to 5,903,333
BT4486
BT4487: BT4487 - putative membrane-bound lytic murein transglycosylase (NCBI ptt file), at 5,903,417 to 5,904,418
BT4487
Position (kb)
5902
5903
5904 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5901.460 kb on - strand, within BT4484 at 5901.460 kb on - strand, within BT4484 at 5901.467 kb on + strand, within BT4484 at 5901.491 kb on + strand, within BT4484 at 5901.516 kb on + strand, within BT4484 at 5901.516 kb on + strand, within BT4484 at 5901.560 kb on + strand, within BT4484 at 5901.711 kb on + strand, within BT4484 at 5901.711 kb on - strand, within BT4484 at 5901.740 kb on - strand, within BT4484 at 5901.836 kb on + strand at 5901.842 kb on + strand at 5901.868 kb on + strand at 5901.868 kb on + strand at 5901.869 kb on - strand at 5901.936 kb on + strand at 5901.940 kb on + strand at 5901.990 kb on + strand at 5901.990 kb on + strand at 5901.991 kb on - strand at 5901.991 kb on - strand at 5902.034 kb on + strand at 5902.050 kb on + strand at 5902.104 kb on - strand, within BT4485 at 5902.125 kb on + strand, within BT4485 at 5902.126 kb on - strand, within BT4485 at 5902.136 kb on + strand, within BT4485 at 5902.144 kb on + strand, within BT4485 at 5902.152 kb on + strand, within BT4485 at 5902.185 kb on + strand, within BT4485 at 5902.199 kb on + strand, within BT4485 at 5902.201 kb on + strand, within BT4485 at 5902.202 kb on - strand, within BT4485 at 5902.366 kb on - strand, within BT4485 at 5902.386 kb on - strand at 5902.456 kb on + strand at 5902.462 kb on - strand at 5902.514 kb on - strand at 5902.699 kb on - strand, within BT4486 at 5902.700 kb on + strand, within BT4486 at 5902.745 kb on + strand, within BT4486 at 5903.003 kb on - strand, within BT4486 at 5903.003 kb on - strand, within BT4486 at 5903.050 kb on - strand, within BT4486 at 5903.053 kb on + strand, within BT4486 at 5903.053 kb on + strand, within BT4486 at 5903.054 kb on - strand, within BT4486 at 5903.078 kb on - strand, within BT4486 at 5903.079 kb on + strand, within BT4486 at 5903.122 kb on + strand, within BT4486 at 5903.190 kb on + strand, within BT4486 at 5903.194 kb on - strand, within BT4486 at 5903.208 kb on - strand, within BT4486 at 5903.272 kb on + strand at 5903.273 kb on - strand at 5903.273 kb on - strand at 5903.280 kb on - strand at 5903.280 kb on - strand at 5903.373 kb on + strand at 5903.400 kb on - strand at 5903.403 kb on + strand at 5903.406 kb on + strand at 5903.406 kb on + strand at 5903.406 kb on + strand at 5903.423 kb on + strand at 5903.440 kb on + strand at 5903.440 kb on + strand at 5903.443 kb on - strand at 5903.466 kb on + strand at 5903.470 kb on - strand at 5903.485 kb on + strand at 5903.572 kb on - strand, within BT4487 at 5903.734 kb on + strand, within BT4487 at 5903.734 kb on + strand, within BT4487 at 5903.735 kb on - strand, within BT4487 at 5903.753 kb on + strand, within BT4487 at 5903.840 kb on + strand, within BT4487 at 5903.840 kb on + strand, within BT4487 at 5903.840 kb on + strand, within BT4487 at 5903.931 kb on - strand, within BT4487 at 5904.017 kb on + strand, within BT4487 at 5904.108 kb on + strand, within BT4487 at 5904.125 kb on - strand, within BT4487 at 5904.152 kb on - strand, within BT4487 at 5904.153 kb on + strand, within BT4487 at 5904.268 kb on - strand, within BT4487 at 5904.325 kb on + strand at 5904.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 5,901,460 - BT4484 0.36 -4.4 5,901,460 - BT4484 0.36 -0.6 5,901,467 + BT4484 0.37 +0.3 5,901,491 + BT4484 0.41 -1.6 5,901,516 + BT4484 0.45 -0.1 5,901,516 + BT4484 0.45 -1.1 5,901,560 + BT4484 0.53 +2.2 5,901,711 + BT4484 0.78 -1.4 5,901,711 - BT4484 0.78 -0.1 5,901,740 - BT4484 0.82 -2.4 5,901,836 + -1.6 5,901,842 + -0.3 5,901,868 + -0.6 5,901,868 + -0.1 5,901,869 - -0.6 5,901,936 + +1.5 5,901,940 + -0.1 5,901,990 + -1.1 5,901,990 + -0.1 5,901,991 - +0.8 5,901,991 - -2.1 5,902,034 + -1.9 5,902,050 + -0.1 5,902,104 - BT4485 0.13 -1.6 5,902,125 + BT4485 0.19 -3.4 5,902,126 - BT4485 0.19 -1.1 5,902,136 + BT4485 0.22 -1.7 5,902,144 + BT4485 0.24 -0.7 5,902,152 + BT4485 0.26 +2.5 5,902,185 + BT4485 0.35 -0.1 5,902,199 + BT4485 0.39 -1.1 5,902,201 + BT4485 0.40 -1.1 5,902,202 - BT4485 0.40 -2.1 5,902,366 - BT4485 0.85 -0.1 5,902,386 - -2.1 5,902,456 + -1.6 5,902,462 - -2.1 5,902,514 - -2.4 5,902,699 - BT4486 0.28 -4.9 5,902,700 + BT4486 0.28 -1.6 5,902,745 + BT4486 0.33 -0.1 5,903,003 - BT4486 0.62 -1.6 5,903,003 - BT4486 0.62 -0.1 5,903,050 - BT4486 0.68 -1.4 5,903,053 + BT4486 0.68 -1.6 5,903,053 + BT4486 0.68 -1.6 5,903,054 - BT4486 0.68 -1.1 5,903,078 - BT4486 0.71 -1.1 5,903,079 + BT4486 0.71 -1.5 5,903,122 + BT4486 0.76 -2.1 5,903,190 + BT4486 0.84 -1.6 5,903,194 - BT4486 0.84 -1.6 5,903,208 - BT4486 0.86 -0.1 5,903,272 + -1.6 5,903,273 - -0.1 5,903,273 - -1.1 5,903,280 - -2.4 5,903,280 - -0.1 5,903,373 + -2.2 5,903,400 - -0.1 5,903,403 + -1.9 5,903,406 + -0.4 5,903,406 + -2.1 5,903,406 + -2.9 5,903,423 + -0.1 5,903,440 + -2.1 5,903,440 + -0.1 5,903,443 - -2.9 5,903,466 + -3.1 5,903,470 - -0.1 5,903,485 + -1.1 5,903,572 - BT4487 0.15 -2.1 5,903,734 + BT4487 0.32 +0.0 5,903,734 + BT4487 0.32 -3.5 5,903,735 - BT4487 0.32 +0.9 5,903,753 + BT4487 0.34 -3.0 5,903,840 + BT4487 0.42 +0.0 5,903,840 + BT4487 0.42 -0.5 5,903,840 + BT4487 0.42 -1.4 5,903,931 - BT4487 0.51 -2.1 5,904,017 + BT4487 0.60 -1.6 5,904,108 + BT4487 0.69 -2.1 5,904,125 - BT4487 0.71 +0.6 5,904,152 - BT4487 0.73 +0.1 5,904,153 + BT4487 0.73 -1.1 5,904,268 - BT4487 0.85 -3.6 5,904,325 + -2.1 5,904,325 + -0.2
Or see this region's nucleotide sequence