Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3322 and BT3323 are separated by 219 nucleotides BT3323 and BT3324 are separated by 231 nucleotides BT3324 and BT3325 are separated by 794 nucleotides
BT3322: BT3322 - putative thioredoxin family protein (NCBI ptt file), at 4,265,719 to 4,268,331
BT3322
BT3323: BT3323 - hypothetical protein (NCBI ptt file), at 4,268,551 to 4,269,072
BT3323
BT3324: BT3324 - chondroitinase (chondroitin lyase) precursor (NCBI ptt file), at 4,269,304 to 4,272,348
BT3324
BT3325: BT3325 - hypothetical protein (NCBI ptt file), at 4,273,143 to 4,274,372
BT3325
Position (kb)
4269
4270
4271
4272
4273 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4268.427 kb on + strand at 4268.431 kb on + strand at 4268.504 kb on + strand at 4268.523 kb on + strand at 4268.593 kb on - strand at 4268.842 kb on + strand, within BT3323 at 4268.846 kb on + strand, within BT3323 at 4268.851 kb on - strand, within BT3323 at 4268.856 kb on + strand, within BT3323 at 4268.897 kb on - strand, within BT3323 at 4268.961 kb on - strand, within BT3323 at 4268.964 kb on - strand, within BT3323 at 4268.990 kb on - strand, within BT3323 at 4269.070 kb on + strand at 4269.234 kb on - strand at 4269.271 kb on - strand at 4269.276 kb on - strand at 4269.288 kb on + strand at 4269.289 kb on - strand at 4269.311 kb on + strand at 4269.311 kb on + strand at 4269.312 kb on - strand at 4269.351 kb on - strand at 4269.354 kb on + strand at 4269.354 kb on + strand at 4269.392 kb on + strand at 4269.393 kb on - strand at 4269.393 kb on - strand at 4269.402 kb on + strand at 4269.448 kb on - strand at 4269.553 kb on + strand at 4269.554 kb on - strand at 4269.580 kb on + strand at 4269.580 kb on + strand at 4269.581 kb on - strand at 4269.989 kb on - strand, within BT3324 at 4269.989 kb on - strand, within BT3324 at 4269.991 kb on - strand, within BT3324 at 4270.060 kb on + strand, within BT3324 at 4270.060 kb on + strand, within BT3324 at 4270.062 kb on + strand at 4270.063 kb on - strand, within BT3324 at 4270.118 kb on - strand, within BT3324 at 4270.149 kb on + strand, within BT3324 at 4270.184 kb on - strand, within BT3324 at 4270.217 kb on - strand, within BT3324 at 4270.217 kb on - strand, within BT3324 at 4270.288 kb on - strand, within BT3324 at 4270.315 kb on - strand, within BT3324 at 4270.329 kb on + strand, within BT3324 at 4270.391 kb on + strand, within BT3324 at 4270.429 kb on - strand, within BT3324 at 4270.441 kb on + strand, within BT3324 at 4270.442 kb on - strand, within BT3324 at 4270.442 kb on - strand, within BT3324 at 4270.447 kb on - strand, within BT3324 at 4270.460 kb on + strand, within BT3324 at 4270.601 kb on - strand, within BT3324 at 4270.671 kb on + strand, within BT3324 at 4270.684 kb on + strand, within BT3324 at 4270.724 kb on - strand, within BT3324 at 4270.882 kb on + strand, within BT3324 at 4271.002 kb on + strand, within BT3324 at 4271.005 kb on - strand, within BT3324 at 4271.034 kb on + strand, within BT3324 at 4271.118 kb on - strand, within BT3324 at 4271.191 kb on + strand, within BT3324 at 4271.192 kb on - strand, within BT3324 at 4271.210 kb on - strand, within BT3324 at 4271.218 kb on + strand, within BT3324 at 4271.219 kb on - strand, within BT3324 at 4271.382 kb on - strand, within BT3324 at 4271.386 kb on - strand, within BT3324 at 4271.386 kb on - strand, within BT3324 at 4271.413 kb on + strand, within BT3324 at 4271.707 kb on + strand, within BT3324 at 4271.708 kb on - strand, within BT3324 at 4271.840 kb on - strand, within BT3324 at 4271.845 kb on + strand, within BT3324 at 4271.846 kb on - strand, within BT3324 at 4271.846 kb on - strand, within BT3324 at 4271.938 kb on - strand, within BT3324 at 4271.938 kb on - strand, within BT3324 at 4271.971 kb on + strand, within BT3324 at 4272.025 kb on - strand, within BT3324 at 4272.030 kb on + strand, within BT3324 at 4272.041 kb on + strand, within BT3324 at 4272.041 kb on + strand, within BT3324 at 4272.041 kb on + strand, within BT3324 at 4272.077 kb on + strand at 4272.077 kb on + strand at 4272.087 kb on - strand at 4272.087 kb on - strand at 4272.109 kb on + strand at 4272.110 kb on - strand at 4272.110 kb on - strand at 4272.154 kb on - strand at 4272.154 kb on - strand at 4272.365 kb on - strand at 4272.401 kb on - strand at 4272.422 kb on - strand at 4272.425 kb on - strand at 4272.449 kb on + strand at 4272.450 kb on - strand at 4272.450 kb on - strand at 4272.526 kb on - strand at 4272.568 kb on + strand at 4272.695 kb on + strand at 4272.714 kb on + strand at 4272.739 kb on + strand at 4272.747 kb on - strand at 4272.747 kb on - strand at 4272.749 kb on + strand at 4272.749 kb on + strand at 4272.750 kb on - strand at 4272.903 kb on - strand at 4272.903 kb on - strand at 4272.910 kb on + strand at 4272.910 kb on + strand at 4272.920 kb on - strand at 4272.996 kb on - strand at 4273.023 kb on - strand at 4273.108 kb on - strand at 4273.172 kb on + strand at 4273.173 kb on - strand at 4273.209 kb on - strand at 4273.343 kb on - strand, within BT3325
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 4,268,427 + +1.2 4,268,431 + -0.8 4,268,504 + +0.2 4,268,523 + -1.0 4,268,593 - -1.4 4,268,842 + BT3323 0.56 -2.2 4,268,846 + BT3323 0.57 +0.2 4,268,851 - BT3323 0.57 -0.8 4,268,856 + BT3323 0.58 -2.2 4,268,897 - BT3323 0.66 -1.3 4,268,961 - BT3323 0.79 -0.3 4,268,964 - BT3323 0.79 -1.4 4,268,990 - BT3323 0.84 -1.2 4,269,070 + -1.1 4,269,234 - -1.1 4,269,271 - -1.4 4,269,276 - -1.8 4,269,288 + -1.2 4,269,289 - +1.0 4,269,311 + -1.1 4,269,311 + +0.4 4,269,312 - -3.5 4,269,351 - -1.8 4,269,354 + -1.8 4,269,354 + -0.8 4,269,392 + -1.4 4,269,393 - -1.4 4,269,393 - +0.2 4,269,402 + +0.2 4,269,448 - -0.8 4,269,553 + +0.2 4,269,554 - +0.2 4,269,580 + -2.3 4,269,580 + -1.0 4,269,581 - -1.4 4,269,989 - BT3324 0.22 -4.2 4,269,989 - BT3324 0.22 -0.2 4,269,991 - BT3324 0.23 +0.2 4,270,060 + BT3324 0.25 -0.6 4,270,060 + BT3324 0.25 +0.2 4,270,062 + +0.2 4,270,063 - BT3324 0.25 -0.3 4,270,118 - BT3324 0.27 +0.7 4,270,149 + BT3324 0.28 -0.8 4,270,184 - BT3324 0.29 -2.8 4,270,217 - BT3324 0.30 -1.8 4,270,217 - BT3324 0.30 +0.2 4,270,288 - BT3324 0.32 -1.4 4,270,315 - BT3324 0.33 +0.2 4,270,329 + BT3324 0.34 +0.2 4,270,391 + BT3324 0.36 -1.6 4,270,429 - BT3324 0.37 +0.2 4,270,441 + BT3324 0.37 -3.4 4,270,442 - BT3324 0.37 -0.6 4,270,442 - BT3324 0.37 +2.0 4,270,447 - BT3324 0.38 +0.2 4,270,460 + BT3324 0.38 -2.4 4,270,601 - BT3324 0.43 -1.8 4,270,671 + BT3324 0.45 +0.1 4,270,684 + BT3324 0.45 -1.4 4,270,724 - BT3324 0.47 -2.6 4,270,882 + BT3324 0.52 -1.8 4,271,002 + BT3324 0.56 -0.8 4,271,005 - BT3324 0.56 +0.4 4,271,034 + BT3324 0.57 -0.8 4,271,118 - BT3324 0.60 +0.2 4,271,191 + BT3324 0.62 +0.2 4,271,192 - BT3324 0.62 -2.2 4,271,210 - BT3324 0.63 +0.2 4,271,218 + BT3324 0.63 -0.8 4,271,219 - BT3324 0.63 +0.2 4,271,382 - BT3324 0.68 +0.2 4,271,386 - BT3324 0.68 +0.6 4,271,386 - BT3324 0.68 -1.4 4,271,413 + BT3324 0.69 -1.9 4,271,707 + BT3324 0.79 -1.8 4,271,708 - BT3324 0.79 +0.2 4,271,840 - BT3324 0.83 -0.8 4,271,845 + BT3324 0.83 -2.8 4,271,846 - BT3324 0.83 +0.2 4,271,846 - BT3324 0.83 +1.6 4,271,938 - BT3324 0.87 -1.1 4,271,938 - BT3324 0.87 -0.9 4,271,971 + BT3324 0.88 +1.1 4,272,025 - BT3324 0.89 -0.6 4,272,030 + BT3324 0.90 +0.5 4,272,041 + BT3324 0.90 +1.2 4,272,041 + BT3324 0.90 -1.3 4,272,041 + BT3324 0.90 -1.4 4,272,077 + +0.8 4,272,077 + -2.2 4,272,087 - -0.6 4,272,087 - -3.3 4,272,109 + -2.6 4,272,110 - -1.0 4,272,110 - -1.8 4,272,154 - +0.3 4,272,154 - -1.4 4,272,365 - -1.0 4,272,401 - -1.4 4,272,422 - +0.2 4,272,425 - -0.2 4,272,449 + -0.8 4,272,450 - -0.5 4,272,450 - -2.2 4,272,526 - +0.2 4,272,568 + -1.8 4,272,695 + +0.4 4,272,714 + -0.8 4,272,739 + -0.8 4,272,747 - -1.4 4,272,747 - -1.6 4,272,749 + +0.2 4,272,749 + -1.8 4,272,750 - -2.4 4,272,903 - -2.6 4,272,903 - -0.4 4,272,910 + -0.8 4,272,910 + -0.6 4,272,920 - -2.2 4,272,996 - -3.2 4,273,023 - -1.4 4,273,108 - -0.4 4,273,172 + -1.8 4,273,173 - +1.0 4,273,209 - -0.8 4,273,343 - BT3325 0.16 -1.0
Or see this region's nucleotide sequence