Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1546

Experiment: BHIS with Simvastatin 30 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1544 and BT1545 are separated by 6 nucleotidesBT1545 and BT1546 are separated by 126 nucleotidesBT1546 and BT1547 are separated by 9 nucleotides BT1544: BT1544 - NADH pyrophosphatase, MutT family hydrolase (NCBI ptt file), at 1,904,740 to 1,905,528 BT1544 BT1545: BT1545 - conserved hypothetical protein, putative regulatory protein with a cAMP-binding domain, (NCBI ptt file), at 1,905,535 to 1,906,098 BT1545 BT1546: BT1546 - acyltransferase (NCBI ptt file), at 1,906,225 to 1,907,232 BT1546 BT1547: BT1547 - conserved hypothetical protein (NCBI ptt file), at 1,907,242 to 1,908,417 BT1547 Position (kb) 1906 1907 1908Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 1905.385 kb on + strand, within BT1544at 1905.523 kb on - strandat 1905.647 kb on + strand, within BT1545at 1905.647 kb on + strand, within BT1545at 1905.647 kb on + strand, within BT1545at 1905.782 kb on + strand, within BT1545at 1905.836 kb on + strand, within BT1545at 1905.836 kb on + strand, within BT1545at 1905.839 kb on - strand, within BT1545at 1905.931 kb on + strand, within BT1545at 1905.941 kb on + strand, within BT1545at 1905.959 kb on + strand, within BT1545at 1905.961 kb on + strand, within BT1545at 1905.962 kb on - strand, within BT1545at 1905.996 kb on - strand, within BT1545at 1906.038 kb on + strand, within BT1545at 1906.041 kb on - strand, within BT1545at 1906.113 kb on - strandat 1906.113 kb on - strandat 1906.126 kb on + strandat 1906.230 kb on - strandat 1906.329 kb on + strand, within BT1546at 1906.401 kb on + strand, within BT1546at 1906.428 kb on - strand, within BT1546at 1906.452 kb on + strand, within BT1546at 1906.453 kb on - strand, within BT1546at 1906.463 kb on - strand, within BT1546at 1906.471 kb on + strand, within BT1546at 1906.471 kb on + strand, within BT1546at 1906.603 kb on + strand, within BT1546at 1906.662 kb on - strand, within BT1546at 1906.696 kb on - strand, within BT1546at 1906.700 kb on - strand, within BT1546at 1906.730 kb on - strand, within BT1546at 1906.730 kb on - strand, within BT1546at 1906.735 kb on + strand, within BT1546at 1906.896 kb on + strand, within BT1546at 1906.897 kb on - strand, within BT1546at 1906.899 kb on - strand, within BT1546at 1906.902 kb on + strand, within BT1546at 1906.908 kb on - strand, within BT1546at 1906.981 kb on - strand, within BT1546at 1907.034 kb on + strand, within BT1546at 1907.062 kb on - strand, within BT1546at 1907.211 kb on - strandat 1907.277 kb on - strandat 1907.279 kb on + strandat 1907.280 kb on - strandat 1907.325 kb on + strandat 1907.403 kb on + strand, within BT1547at 1907.445 kb on - strand, within BT1547at 1907.467 kb on - strand, within BT1547at 1907.476 kb on - strand, within BT1547at 1907.543 kb on + strand, within BT1547at 1907.632 kb on - strand, within BT1547at 1907.632 kb on - strand, within BT1547at 1907.658 kb on + strand, within BT1547at 1907.664 kb on - strand, within BT1547at 1907.674 kb on - strand, within BT1547at 1907.738 kb on - strand, within BT1547at 1907.738 kb on - strand, within BT1547at 1907.770 kb on + strand, within BT1547at 1907.838 kb on - strand, within BT1547at 1907.838 kb on - strand, within BT1547at 1908.068 kb on + strand, within BT1547at 1908.084 kb on + strand, within BT1547at 1908.105 kb on + strand, within BT1547at 1908.106 kb on - strand, within BT1547at 1908.151 kb on - strand, within BT1547at 1908.209 kb on - strand, within BT1547at 1908.222 kb on + strand, within BT1547at 1908.226 kb on - strand, within BT1547

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM
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1,905,385 + BT1544 0.82 -0.5
1,905,523 - -2.2
1,905,647 + BT1545 0.20 -0.1
1,905,647 + BT1545 0.20 -1.2
1,905,647 + BT1545 0.20 -1.8
1,905,782 + BT1545 0.44 -0.2
1,905,836 + BT1545 0.53 -0.0
1,905,836 + BT1545 0.53 -2.2
1,905,839 - BT1545 0.54 -0.0
1,905,931 + BT1545 0.70 -2.5
1,905,941 + BT1545 0.72 +0.6
1,905,959 + BT1545 0.75 -0.2
1,905,961 + BT1545 0.76 -0.9
1,905,962 - BT1545 0.76 -1.5
1,905,996 - BT1545 0.82 -0.2
1,906,038 + BT1545 0.89 -4.9
1,906,041 - BT1545 0.90 -1.8
1,906,113 - -1.2
1,906,113 - -2.2
1,906,126 + +1.1
1,906,230 - -1.8
1,906,329 + BT1546 0.10 -1.2
1,906,401 + BT1546 0.17 -1.2
1,906,428 - BT1546 0.20 -1.2
1,906,452 + BT1546 0.23 -1.2
1,906,453 - BT1546 0.23 -1.2
1,906,463 - BT1546 0.24 -0.2
1,906,471 + BT1546 0.24 -0.2
1,906,471 + BT1546 0.24 -0.7
1,906,603 + BT1546 0.38 -0.2
1,906,662 - BT1546 0.43 -0.2
1,906,696 - BT1546 0.47 -0.2
1,906,700 - BT1546 0.47 -3.4
1,906,730 - BT1546 0.50 -3.0
1,906,730 - BT1546 0.50 -1.6
1,906,735 + BT1546 0.51 -2.0
1,906,896 + BT1546 0.67 -2.0
1,906,897 - BT1546 0.67 +1.6
1,906,899 - BT1546 0.67 -3.0
1,906,902 + BT1546 0.67 -2.7
1,906,908 - BT1546 0.68 -1.8
1,906,981 - BT1546 0.75 -3.0
1,907,034 + BT1546 0.80 -2.5
1,907,062 - BT1546 0.83 -0.9
1,907,211 - -1.8
1,907,277 - +0.0
1,907,279 + -2.4
1,907,280 - -0.2
1,907,325 + +1.0
1,907,403 + BT1547 0.14 -3.0
1,907,445 - BT1547 0.17 -0.5
1,907,467 - BT1547 0.19 +1.8
1,907,476 - BT1547 0.20 -0.5
1,907,543 + BT1547 0.26 -0.4
1,907,632 - BT1547 0.33 -1.5
1,907,632 - BT1547 0.33 -0.2
1,907,658 + BT1547 0.35 -1.8
1,907,664 - BT1547 0.36 -0.2
1,907,674 - BT1547 0.37 -2.5
1,907,738 - BT1547 0.42 +3.0
1,907,738 - BT1547 0.42 -2.5
1,907,770 + BT1547 0.45 -2.2
1,907,838 - BT1547 0.51 -1.2
1,907,838 - BT1547 0.51 +0.7
1,908,068 + BT1547 0.70 -1.4
1,908,084 + BT1547 0.72 -0.2
1,908,105 + BT1547 0.73 +0.4
1,908,106 - BT1547 0.73 -3.0
1,908,151 - BT1547 0.77 -3.4
1,908,209 - BT1547 0.82 -1.8
1,908,222 + BT1547 0.83 -2.8
1,908,226 - BT1547 0.84 -2.2

Or see this region's nucleotide sequence