Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1544 and BT1545 are separated by 6 nucleotides BT1545 and BT1546 are separated by 126 nucleotides BT1546 and BT1547 are separated by 9 nucleotides
BT1544: BT1544 - NADH pyrophosphatase, MutT family hydrolase (NCBI ptt file), at 1,904,740 to 1,905,528
BT1544
BT1545: BT1545 - conserved hypothetical protein, putative regulatory protein with a cAMP-binding domain, (NCBI ptt file), at 1,905,535 to 1,906,098
BT1545
BT1546: BT1546 - acyltransferase (NCBI ptt file), at 1,906,225 to 1,907,232
BT1546
BT1547: BT1547 - conserved hypothetical protein (NCBI ptt file), at 1,907,242 to 1,908,417
BT1547
Position (kb)
1906
1907
1908 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1905.385 kb on + strand, within BT1544 at 1905.523 kb on - strand at 1905.647 kb on + strand, within BT1545 at 1905.647 kb on + strand, within BT1545 at 1905.647 kb on + strand, within BT1545 at 1905.782 kb on + strand, within BT1545 at 1905.836 kb on + strand, within BT1545 at 1905.836 kb on + strand, within BT1545 at 1905.839 kb on - strand, within BT1545 at 1905.931 kb on + strand, within BT1545 at 1905.941 kb on + strand, within BT1545 at 1905.959 kb on + strand, within BT1545 at 1905.961 kb on + strand, within BT1545 at 1905.962 kb on - strand, within BT1545 at 1905.996 kb on - strand, within BT1545 at 1906.038 kb on + strand, within BT1545 at 1906.041 kb on - strand, within BT1545 at 1906.113 kb on - strand at 1906.113 kb on - strand at 1906.126 kb on + strand at 1906.230 kb on - strand at 1906.329 kb on + strand, within BT1546 at 1906.401 kb on + strand, within BT1546 at 1906.428 kb on - strand, within BT1546 at 1906.452 kb on + strand, within BT1546 at 1906.453 kb on - strand, within BT1546 at 1906.463 kb on - strand, within BT1546 at 1906.471 kb on + strand, within BT1546 at 1906.471 kb on + strand, within BT1546 at 1906.603 kb on + strand, within BT1546 at 1906.662 kb on - strand, within BT1546 at 1906.696 kb on - strand, within BT1546 at 1906.700 kb on - strand, within BT1546 at 1906.730 kb on - strand, within BT1546 at 1906.730 kb on - strand, within BT1546 at 1906.735 kb on + strand, within BT1546 at 1906.896 kb on + strand, within BT1546 at 1906.897 kb on - strand, within BT1546 at 1906.899 kb on - strand, within BT1546 at 1906.902 kb on + strand, within BT1546 at 1906.908 kb on - strand, within BT1546 at 1906.981 kb on - strand, within BT1546 at 1907.034 kb on + strand, within BT1546 at 1907.062 kb on - strand, within BT1546 at 1907.211 kb on - strand at 1907.277 kb on - strand at 1907.279 kb on + strand at 1907.280 kb on - strand at 1907.325 kb on + strand at 1907.403 kb on + strand, within BT1547 at 1907.445 kb on - strand, within BT1547 at 1907.467 kb on - strand, within BT1547 at 1907.476 kb on - strand, within BT1547 at 1907.543 kb on + strand, within BT1547 at 1907.632 kb on - strand, within BT1547 at 1907.632 kb on - strand, within BT1547 at 1907.658 kb on + strand, within BT1547 at 1907.664 kb on - strand, within BT1547 at 1907.674 kb on - strand, within BT1547 at 1907.738 kb on - strand, within BT1547 at 1907.738 kb on - strand, within BT1547 at 1907.770 kb on + strand, within BT1547 at 1907.838 kb on - strand, within BT1547 at 1907.838 kb on - strand, within BT1547 at 1908.068 kb on + strand, within BT1547 at 1908.084 kb on + strand, within BT1547 at 1908.105 kb on + strand, within BT1547 at 1908.106 kb on - strand, within BT1547 at 1908.151 kb on - strand, within BT1547 at 1908.209 kb on - strand, within BT1547 at 1908.222 kb on + strand, within BT1547 at 1908.226 kb on - strand, within BT1547
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,905,385 + BT1544 0.82 -0.5 1,905,523 - -2.2 1,905,647 + BT1545 0.20 -0.1 1,905,647 + BT1545 0.20 -1.2 1,905,647 + BT1545 0.20 -1.8 1,905,782 + BT1545 0.44 -0.2 1,905,836 + BT1545 0.53 -0.0 1,905,836 + BT1545 0.53 -2.2 1,905,839 - BT1545 0.54 -0.0 1,905,931 + BT1545 0.70 -2.5 1,905,941 + BT1545 0.72 +0.6 1,905,959 + BT1545 0.75 -0.2 1,905,961 + BT1545 0.76 -0.9 1,905,962 - BT1545 0.76 -1.5 1,905,996 - BT1545 0.82 -0.2 1,906,038 + BT1545 0.89 -4.9 1,906,041 - BT1545 0.90 -1.8 1,906,113 - -1.2 1,906,113 - -2.2 1,906,126 + +1.1 1,906,230 - -1.8 1,906,329 + BT1546 0.10 -1.2 1,906,401 + BT1546 0.17 -1.2 1,906,428 - BT1546 0.20 -1.2 1,906,452 + BT1546 0.23 -1.2 1,906,453 - BT1546 0.23 -1.2 1,906,463 - BT1546 0.24 -0.2 1,906,471 + BT1546 0.24 -0.2 1,906,471 + BT1546 0.24 -0.7 1,906,603 + BT1546 0.38 -0.2 1,906,662 - BT1546 0.43 -0.2 1,906,696 - BT1546 0.47 -0.2 1,906,700 - BT1546 0.47 -3.4 1,906,730 - BT1546 0.50 -3.0 1,906,730 - BT1546 0.50 -1.6 1,906,735 + BT1546 0.51 -2.0 1,906,896 + BT1546 0.67 -2.0 1,906,897 - BT1546 0.67 +1.6 1,906,899 - BT1546 0.67 -3.0 1,906,902 + BT1546 0.67 -2.7 1,906,908 - BT1546 0.68 -1.8 1,906,981 - BT1546 0.75 -3.0 1,907,034 + BT1546 0.80 -2.5 1,907,062 - BT1546 0.83 -0.9 1,907,211 - -1.8 1,907,277 - +0.0 1,907,279 + -2.4 1,907,280 - -0.2 1,907,325 + +1.0 1,907,403 + BT1547 0.14 -3.0 1,907,445 - BT1547 0.17 -0.5 1,907,467 - BT1547 0.19 +1.8 1,907,476 - BT1547 0.20 -0.5 1,907,543 + BT1547 0.26 -0.4 1,907,632 - BT1547 0.33 -1.5 1,907,632 - BT1547 0.33 -0.2 1,907,658 + BT1547 0.35 -1.8 1,907,664 - BT1547 0.36 -0.2 1,907,674 - BT1547 0.37 -2.5 1,907,738 - BT1547 0.42 +3.0 1,907,738 - BT1547 0.42 -2.5 1,907,770 + BT1547 0.45 -2.2 1,907,838 - BT1547 0.51 -1.2 1,907,838 - BT1547 0.51 +0.7 1,908,068 + BT1547 0.70 -1.4 1,908,084 + BT1547 0.72 -0.2 1,908,105 + BT1547 0.73 +0.4 1,908,106 - BT1547 0.73 -3.0 1,908,151 - BT1547 0.77 -3.4 1,908,209 - BT1547 0.82 -1.8 1,908,222 + BT1547 0.83 -2.8 1,908,226 - BT1547 0.84 -2.2
Or see this region's nucleotide sequence