Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4420 and BT4421 are separated by 56 nucleotides BT4421 and BT4422 are separated by 13 nucleotides BT4422 and BT4423 are separated by 28 nucleotides
BT4420: BT4420 - conserved hypothetical protein, with a conserved domain (NCBI ptt file), at 5,823,549 to 5,824,637
BT4420
BT4421: BT4421 - conserved hypothetical protein (NCBI ptt file), at 5,824,694 to 5,825,065
BT4421
BT4422: BT4422 - conserved hypothetical protein (NCBI ptt file), at 5,825,079 to 5,825,558
BT4422
BT4423: BT4423 - xylulose kinase (NCBI ptt file), at 5,825,587 to 5,827,104
BT4423
Position (kb)
5824
5825
5826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5823.789 kb on - strand, within BT4420 at 5823.789 kb on - strand, within BT4420 at 5823.992 kb on + strand, within BT4420 at 5823.993 kb on - strand, within BT4420 at 5824.052 kb on - strand, within BT4420 at 5824.054 kb on - strand, within BT4420 at 5824.116 kb on + strand, within BT4420 at 5824.158 kb on - strand, within BT4420 at 5824.389 kb on - strand, within BT4420 at 5824.586 kb on - strand at 5824.636 kb on - strand at 5824.666 kb on - strand at 5824.688 kb on + strand at 5824.689 kb on - strand at 5824.700 kb on + strand at 5824.701 kb on - strand at 5824.701 kb on - strand at 5824.704 kb on - strand at 5824.778 kb on + strand, within BT4421 at 5824.832 kb on + strand, within BT4421 at 5824.834 kb on + strand, within BT4421 at 5824.976 kb on - strand, within BT4421 at 5825.020 kb on - strand, within BT4421 at 5825.172 kb on - strand, within BT4422 at 5825.172 kb on - strand, within BT4422 at 5825.490 kb on - strand, within BT4422 at 5825.566 kb on - strand at 5825.584 kb on - strand at 5825.592 kb on + strand at 5825.592 kb on + strand at 5825.692 kb on + strand at 5825.693 kb on - strand at 5825.801 kb on + strand, within BT4423 at 5825.842 kb on - strand, within BT4423 at 5825.876 kb on - strand, within BT4423 at 5825.876 kb on - strand, within BT4423 at 5826.059 kb on - strand, within BT4423
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 5,823,789 - BT4420 0.22 -0.1 5,823,789 - BT4420 0.22 +2.1 5,823,992 + BT4420 0.41 -2.1 5,823,993 - BT4420 0.41 -0.1 5,824,052 - BT4420 0.46 -2.9 5,824,054 - BT4420 0.46 -1.7 5,824,116 + BT4420 0.52 -1.1 5,824,158 - BT4420 0.56 -1.7 5,824,389 - BT4420 0.77 -2.1 5,824,586 - -3.6 5,824,636 - -0.1 5,824,666 - +2.0 5,824,688 + -0.1 5,824,689 - -1.1 5,824,700 + +1.9 5,824,701 - -2.4 5,824,701 - -0.3 5,824,704 - -0.1 5,824,778 + BT4421 0.23 -1.7 5,824,832 + BT4421 0.37 -0.1 5,824,834 + BT4421 0.38 +2.1 5,824,976 - BT4421 0.76 -0.1 5,825,020 - BT4421 0.88 -1.1 5,825,172 - BT4422 0.19 -0.6 5,825,172 - BT4422 0.19 -1.7 5,825,490 - BT4422 0.86 -2.9 5,825,566 - -1.1 5,825,584 - -3.4 5,825,592 + -3.4 5,825,592 + +1.6 5,825,692 + +0.9 5,825,693 - -2.1 5,825,801 + BT4423 0.14 -1.7 5,825,842 - BT4423 0.17 +0.6 5,825,876 - BT4423 0.19 +0.1 5,825,876 - BT4423 0.19 -2.1 5,826,059 - BT4423 0.31 -0.1
Or see this region's nucleotide sequence