Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4339 and BT4340 are separated by 182 nucleotides BT4340 and BT4341 are separated by 428 nucleotides BT4341 and BT4342 are separated by 205 nucleotides
BT4339: BT4339 - glutamine synthetase (NCBI ptt file), at 5,714,701 to 5,716,890
BT4339
BT4340: BT4340 - hypothetical protein (NCBI ptt file), at 5,717,073 to 5,717,273
BT4340
BT4341: BT4341 - hypothetical protein (NCBI ptt file), at 5,717,702 to 5,719,861
BT4341
BT4342: BT4342 - RNA-directed DNA polymerase (NCBI ptt file), at 5,720,067 to 5,721,200
BT4342
Position (kb)
5717
5718
5719
5720 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5716.761 kb on - strand at 5717.064 kb on + strand at 5717.067 kb on - strand at 5717.154 kb on + strand, within BT4340 at 5717.169 kb on - strand, within BT4340 at 5717.170 kb on + strand, within BT4340 at 5717.170 kb on + strand, within BT4340 at 5717.171 kb on - strand, within BT4340 at 5717.211 kb on - strand, within BT4340 at 5717.241 kb on + strand, within BT4340 at 5717.380 kb on + strand at 5717.381 kb on - strand at 5717.425 kb on - strand at 5717.458 kb on + strand at 5717.461 kb on + strand at 5717.462 kb on - strand at 5717.527 kb on + strand at 5717.535 kb on - strand at 5717.668 kb on - strand at 5717.683 kb on + strand at 5717.688 kb on + strand at 5717.702 kb on - strand at 5717.702 kb on - strand at 5717.753 kb on - strand at 5717.761 kb on + strand at 5717.856 kb on + strand at 5717.856 kb on + strand at 5717.856 kb on + strand at 5717.858 kb on + strand at 5717.860 kb on + strand at 5717.863 kb on - strand at 5717.948 kb on + strand, within BT4341 at 5717.966 kb on - strand, within BT4341 at 5717.981 kb on - strand, within BT4341 at 5717.997 kb on - strand, within BT4341 at 5718.071 kb on - strand, within BT4341 at 5718.083 kb on + strand, within BT4341 at 5718.084 kb on - strand, within BT4341 at 5718.155 kb on + strand, within BT4341 at 5718.250 kb on + strand, within BT4341 at 5718.272 kb on - strand, within BT4341 at 5718.376 kb on - strand at 5718.380 kb on + strand, within BT4341 at 5718.400 kb on + strand, within BT4341 at 5718.416 kb on - strand, within BT4341 at 5718.432 kb on - strand, within BT4341 at 5718.633 kb on - strand, within BT4341 at 5718.786 kb on - strand, within BT4341 at 5718.883 kb on + strand, within BT4341 at 5718.906 kb on - strand, within BT4341 at 5718.989 kb on + strand, within BT4341 at 5719.096 kb on + strand, within BT4341 at 5719.234 kb on + strand, within BT4341 at 5719.238 kb on + strand, within BT4341 at 5719.287 kb on - strand, within BT4341 at 5719.290 kb on - strand, within BT4341 at 5719.294 kb on + strand, within BT4341 at 5719.353 kb on - strand, within BT4341 at 5719.404 kb on + strand, within BT4341 at 5719.424 kb on - strand, within BT4341 at 5719.424 kb on - strand, within BT4341 at 5719.499 kb on + strand, within BT4341 at 5719.595 kb on + strand, within BT4341 at 5719.596 kb on - strand, within BT4341 at 5719.596 kb on - strand, within BT4341 at 5719.617 kb on + strand, within BT4341 at 5719.674 kb on - strand at 5719.706 kb on - strand at 5719.883 kb on + strand at 5719.884 kb on - strand at 5719.884 kb on - strand at 5719.892 kb on - strand at 5719.893 kb on - strand at 5719.897 kb on + strand at 5719.898 kb on - strand at 5719.910 kb on - strand at 5719.941 kb on - strand at 5719.949 kb on + strand at 5719.949 kb on + strand at 5719.993 kb on + strand at 5719.994 kb on - strand at 5720.003 kb on + strand at 5720.052 kb on - strand at 5720.054 kb on - strand at 5720.085 kb on - strand at 5720.090 kb on - strand at 5720.093 kb on + strand at 5720.094 kb on - strand at 5720.151 kb on + strand at 5720.187 kb on - strand, within BT4342 at 5720.190 kb on - strand at 5720.245 kb on + strand, within BT4342 at 5720.460 kb on - strand, within BT4342 at 5720.463 kb on + strand, within BT4342 at 5720.464 kb on - strand, within BT4342 at 5720.471 kb on + strand, within BT4342 at 5720.473 kb on + strand, within BT4342 at 5720.473 kb on + strand, within BT4342 at 5720.474 kb on - strand, within BT4342 at 5720.474 kb on - strand, within BT4342 at 5720.474 kb on - strand, within BT4342 at 5720.476 kb on + strand, within BT4342 at 5720.485 kb on + strand, within BT4342 at 5720.565 kb on + strand, within BT4342 at 5720.658 kb on - strand, within BT4342 at 5720.687 kb on + strand, within BT4342 at 5720.799 kb on + strand, within BT4342 at 5720.809 kb on - strand, within BT4342 at 5720.822 kb on + strand, within BT4342
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 5,716,761 - -0.1 5,717,064 + -0.1 5,717,067 - -0.1 5,717,154 + BT4340 0.40 -0.4 5,717,169 - BT4340 0.48 -0.0 5,717,170 + BT4340 0.48 -0.1 5,717,170 + BT4340 0.48 -2.1 5,717,171 - BT4340 0.49 +0.4 5,717,211 - BT4340 0.69 -1.7 5,717,241 + BT4340 0.84 -2.1 5,717,380 + -1.1 5,717,381 - -0.1 5,717,425 - +1.3 5,717,458 + -2.1 5,717,461 + -1.7 5,717,462 - -3.5 5,717,527 + -0.1 5,717,535 - -2.1 5,717,668 - +1.5 5,717,683 + -1.7 5,717,688 + +0.9 5,717,702 - +1.6 5,717,702 - +1.7 5,717,753 - -2.9 5,717,761 + +1.5 5,717,856 + -2.1 5,717,856 + -2.1 5,717,856 + -1.1 5,717,858 + -2.9 5,717,860 + -1.7 5,717,863 - -1.1 5,717,948 + BT4341 0.11 -1.7 5,717,966 - BT4341 0.12 -1.1 5,717,981 - BT4341 0.13 -0.1 5,717,997 - BT4341 0.14 -1.1 5,718,071 - BT4341 0.17 -1.3 5,718,083 + BT4341 0.18 -0.1 5,718,084 - BT4341 0.18 +0.1 5,718,155 + BT4341 0.21 +1.9 5,718,250 + BT4341 0.25 +0.7 5,718,272 - BT4341 0.26 -1.1 5,718,376 - -0.1 5,718,380 + BT4341 0.31 -1.1 5,718,400 + BT4341 0.32 -1.7 5,718,416 - BT4341 0.33 -3.4 5,718,432 - BT4341 0.34 +1.1 5,718,633 - BT4341 0.43 +1.4 5,718,786 - BT4341 0.50 -1.1 5,718,883 + BT4341 0.55 +0.5 5,718,906 - BT4341 0.56 -0.1 5,718,989 + BT4341 0.60 -0.1 5,719,096 + BT4341 0.65 -1.1 5,719,234 + BT4341 0.71 +1.1 5,719,238 + BT4341 0.71 -0.1 5,719,287 - BT4341 0.73 +1.8 5,719,290 - BT4341 0.74 -0.7 5,719,294 + BT4341 0.74 -2.4 5,719,353 - BT4341 0.76 -2.9 5,719,404 + BT4341 0.79 -2.4 5,719,424 - BT4341 0.80 -0.1 5,719,424 - BT4341 0.80 -1.1 5,719,499 + BT4341 0.83 -0.1 5,719,595 + BT4341 0.88 -0.9 5,719,596 - BT4341 0.88 -1.7 5,719,596 - BT4341 0.88 -1.8 5,719,617 + BT4341 0.89 -1.7 5,719,674 - -0.1 5,719,706 - +0.5 5,719,883 + -2.4 5,719,884 - -0.1 5,719,884 - -0.1 5,719,892 - -1.1 5,719,893 - -2.1 5,719,897 + +2.1 5,719,898 - -2.1 5,719,910 - -2.1 5,719,941 - -1.1 5,719,949 + -1.1 5,719,949 + -0.1 5,719,993 + -1.7 5,719,994 - -1.1 5,720,003 + +0.3 5,720,052 - -1.7 5,720,054 - -1.4 5,720,085 - -1.1 5,720,090 - -0.1 5,720,093 + -1.3 5,720,094 - -0.1 5,720,151 + -1.7 5,720,187 - BT4342 0.11 -1.1 5,720,190 - -0.1 5,720,245 + BT4342 0.16 -1.7 5,720,460 - BT4342 0.35 -0.1 5,720,463 + BT4342 0.35 -2.1 5,720,464 - BT4342 0.35 -1.3 5,720,471 + BT4342 0.36 -1.1 5,720,473 + BT4342 0.36 -0.1 5,720,473 + BT4342 0.36 -0.1 5,720,474 - BT4342 0.36 -0.1 5,720,474 - BT4342 0.36 -1.9 5,720,474 - BT4342 0.36 -1.1 5,720,476 + BT4342 0.36 -1.7 5,720,485 + BT4342 0.37 -2.9 5,720,565 + BT4342 0.44 -1.1 5,720,658 - BT4342 0.52 -4.3 5,720,687 + BT4342 0.55 -0.1 5,720,799 + BT4342 0.65 -3.3 5,720,809 - BT4342 0.65 -0.1 5,720,822 + BT4342 0.67 -1.3
Or see this region's nucleotide sequence