Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4207 and BT4208 are separated by 81 nucleotides BT4208 and BT4209 are separated by 9 nucleotides BT4209 and BT4210 are separated by 16 nucleotides
BT4207: BT4207 - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (NCBI ptt file), at 5,543,651 to 5,544,691
BT4207
BT4208: BT4208 - phosphohydrolase (NCBI ptt file), at 5,544,773 to 5,546,002
BT4208
BT4209: BT4209 - orotidine 5'-phosphate decarboxylase (NCBI ptt file), at 5,546,012 to 5,546,836
BT4209
BT4210: BT4210 - peptide chain release factor 1 (NCBI ptt file), at 5,546,853 to 5,547,965
BT4210
Position (kb)
5544
5545
5546
5547 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5544.775 kb on - strand at 5544.775 kb on - strand at 5544.775 kb on - strand at 5544.775 kb on - strand at 5544.802 kb on - strand at 5544.802 kb on - strand at 5544.802 kb on - strand at 5544.872 kb on - strand at 5544.886 kb on - strand at 5544.890 kb on - strand at 5544.893 kb on - strand at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5544.919 kb on - strand, within BT4208 at 5545.014 kb on - strand, within BT4208 at 5545.085 kb on - strand, within BT4208 at 5545.122 kb on - strand, within BT4208 at 5545.219 kb on - strand, within BT4208 at 5545.219 kb on - strand, within BT4208 at 5545.301 kb on - strand at 5545.486 kb on - strand, within BT4208 at 5545.598 kb on - strand, within BT4208 at 5545.679 kb on - strand, within BT4208 at 5545.739 kb on + strand, within BT4208 at 5545.764 kb on - strand, within BT4208 at 5545.766 kb on - strand, within BT4208 at 5545.766 kb on - strand, within BT4208 at 5545.843 kb on + strand, within BT4208 at 5545.844 kb on - strand, within BT4208 at 5545.844 kb on - strand, within BT4208 at 5545.862 kb on + strand, within BT4208 at 5545.863 kb on - strand, within BT4208 at 5545.863 kb on - strand, within BT4208 at 5545.881 kb on - strand at 5545.928 kb on - strand at 5545.983 kb on - strand at 5545.995 kb on + strand at 5546.237 kb on - strand, within BT4209 at 5546.288 kb on - strand, within BT4209 at 5546.406 kb on - strand, within BT4209 at 5546.464 kb on - strand, within BT4209 at 5546.518 kb on - strand, within BT4209 at 5546.841 kb on - strand at 5546.841 kb on - strand at 5546.841 kb on - strand at 5546.843 kb on - strand at 5546.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 5,544,775 - +1.2 5,544,775 - -0.0 5,544,775 - -2.6 5,544,775 - -0.6 5,544,802 - +1.5 5,544,802 - -1.6 5,544,802 - +2.2 5,544,872 - +2.5 5,544,886 - +2.8 5,544,890 - +1.8 5,544,893 - -2.0 5,544,919 - BT4208 0.12 -2.3 5,544,919 - BT4208 0.12 +3.3 5,544,919 - BT4208 0.12 -1.6 5,544,919 - BT4208 0.12 +3.2 5,544,919 - BT4208 0.12 +2.1 5,545,014 - BT4208 0.20 +3.7 5,545,085 - BT4208 0.25 +0.9 5,545,122 - BT4208 0.28 -0.0 5,545,219 - BT4208 0.36 -0.0 5,545,219 - BT4208 0.36 +1.4 5,545,301 - -0.0 5,545,486 - BT4208 0.58 +1.9 5,545,598 - BT4208 0.67 +0.8 5,545,679 - BT4208 0.74 +1.5 5,545,739 + BT4208 0.79 -2.6 5,545,764 - BT4208 0.81 +0.4 5,545,766 - BT4208 0.81 +2.0 5,545,766 - BT4208 0.81 -0.5 5,545,843 + BT4208 0.87 -4.3 5,545,844 - BT4208 0.87 -1.6 5,545,844 - BT4208 0.87 -3.2 5,545,862 + BT4208 0.89 -3.5 5,545,863 - BT4208 0.89 +1.6 5,545,863 - BT4208 0.89 -1.6 5,545,881 - -1.2 5,545,928 - -2.0 5,545,983 - -2.6 5,545,995 + -4.8 5,546,237 - BT4209 0.27 -1.6 5,546,288 - BT4209 0.33 -1.6 5,546,406 - BT4209 0.48 -0.0 5,546,464 - BT4209 0.55 -2.3 5,546,518 - BT4209 0.61 -1.0 5,546,841 - -3.6 5,546,841 - -1.0 5,546,841 - -1.6 5,546,843 - -0.0 5,546,846 - +0.6
Or see this region's nucleotide sequence