Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2519

Experiment: BHIS with Simvastatin 30 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2517 and BT2518 are separated by 0 nucleotidesBT2518 and BT2519 are separated by 81 nucleotidesBT2519 and BT2520 are separated by 30 nucleotidesBT2520 and BT2521 are separated by 41 nucleotides BT2517: BT2517 - GcpE, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (NCBI ptt file), at 3,141,356 to 3,143,197 BT2517 BT2518: BT2518 - phosphoribosylaminoimidazole carboxylase (NCBI ptt file), at 3,143,198 to 3,143,713 BT2518 BT2519: BT2519 - putative glycine cleavage system H protein (NCBI ptt file), at 3,143,795 to 3,144,175 BT2519 BT2520: BT2520 - conserved hypothetical protein (NCBI ptt file), at 3,144,206 to 3,144,877 BT2520 BT2521: BT2521 - RNA polymerase sigma-54 (NCBI ptt file), at 3,144,919 to 3,146,403 BT2521 Position (kb) 3143 3144 3145Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3143.529 kb on - strand, within BT2518at 3143.728 kb on - strandat 3143.731 kb on - strandat 3143.735 kb on - strandat 3143.780 kb on - strandat 3144.050 kb on - strand, within BT2519at 3144.060 kb on - strand, within BT2519at 3144.060 kb on - strand, within BT2519at 3144.098 kb on - strand, within BT2519at 3144.102 kb on - strand, within BT2519at 3144.102 kb on - strand, within BT2519at 3144.102 kb on - strand, within BT2519at 3144.102 kb on - strand, within BT2519at 3144.182 kb on - strandat 3144.197 kb on - strandat 3144.197 kb on - strandat 3144.386 kb on - strandat 3144.527 kb on - strand, within BT2520at 3144.548 kb on - strand, within BT2520at 3144.574 kb on - strand, within BT2520at 3144.600 kb on - strand, within BT2520at 3144.600 kb on - strandat 3144.600 kb on - strand, within BT2520at 3144.600 kb on - strand, within BT2520at 3144.600 kb on - strand, within BT2520at 3144.604 kb on - strand, within BT2520at 3144.604 kb on - strand, within BT2520at 3144.687 kb on - strand, within BT2520at 3144.727 kb on - strand, within BT2520at 3144.727 kb on - strand, within BT2520at 3144.791 kb on - strand, within BT2520at 3144.803 kb on - strand, within BT2520at 3144.889 kb on - strandat 3144.889 kb on - strandat 3144.896 kb on - strandat 3144.901 kb on - strandat 3144.915 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM
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3,143,529 - BT2518 0.64 +0.1
3,143,728 - -1.5
3,143,731 - -2.2
3,143,735 - -1.1
3,143,780 - +1.8
3,144,050 - BT2519 0.67 +1.5
3,144,060 - BT2519 0.70 -1.5
3,144,060 - BT2519 0.70 -0.2
3,144,098 - BT2519 0.80 -4.1
3,144,102 - BT2519 0.81 -0.5
3,144,102 - BT2519 0.81 -0.8
3,144,102 - BT2519 0.81 +0.3
3,144,102 - BT2519 0.81 -2.5
3,144,182 - -1.5
3,144,197 - -0.7
3,144,197 - -1.5
3,144,386 - +0.7
3,144,527 - BT2520 0.48 +0.1
3,144,548 - BT2520 0.51 +1.0
3,144,574 - BT2520 0.55 -2.2
3,144,600 - BT2520 0.59 -0.9
3,144,600 - -0.9
3,144,600 - BT2520 0.59 -0.7
3,144,600 - BT2520 0.59 +1.8
3,144,600 - BT2520 0.59 -2.7
3,144,604 - BT2520 0.59 -1.9
3,144,604 - BT2520 0.59 -1.2
3,144,687 - BT2520 0.72 +1.1
3,144,727 - BT2520 0.78 -1.9
3,144,727 - BT2520 0.78 -1.5
3,144,791 - BT2520 0.87 -1.1
3,144,803 - BT2520 0.89 -0.9
3,144,889 - -1.9
3,144,889 - -1.9
3,144,896 - +0.1
3,144,901 - +0.7
3,144,915 - -2.9

Or see this region's nucleotide sequence