Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2517 and BT2518 are separated by 0 nucleotides BT2518 and BT2519 are separated by 81 nucleotides BT2519 and BT2520 are separated by 30 nucleotides BT2520 and BT2521 are separated by 41 nucleotides
BT2517: BT2517 - GcpE, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (NCBI ptt file), at 3,141,356 to 3,143,197
BT2517
BT2518: BT2518 - phosphoribosylaminoimidazole carboxylase (NCBI ptt file), at 3,143,198 to 3,143,713
BT2518
BT2519: BT2519 - putative glycine cleavage system H protein (NCBI ptt file), at 3,143,795 to 3,144,175
BT2519
BT2520: BT2520 - conserved hypothetical protein (NCBI ptt file), at 3,144,206 to 3,144,877
BT2520
BT2521: BT2521 - RNA polymerase sigma-54 (NCBI ptt file), at 3,144,919 to 3,146,403
BT2521
Position (kb)
3143
3144
3145 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3143.529 kb on - strand, within BT2518 at 3143.728 kb on - strand at 3143.731 kb on - strand at 3143.735 kb on - strand at 3143.780 kb on - strand at 3144.050 kb on - strand, within BT2519 at 3144.060 kb on - strand, within BT2519 at 3144.060 kb on - strand, within BT2519 at 3144.098 kb on - strand, within BT2519 at 3144.102 kb on - strand, within BT2519 at 3144.102 kb on - strand, within BT2519 at 3144.102 kb on - strand, within BT2519 at 3144.102 kb on - strand, within BT2519 at 3144.182 kb on - strand at 3144.197 kb on - strand at 3144.197 kb on - strand at 3144.386 kb on - strand at 3144.527 kb on - strand, within BT2520 at 3144.548 kb on - strand, within BT2520 at 3144.574 kb on - strand, within BT2520 at 3144.600 kb on - strand, within BT2520 at 3144.600 kb on - strand at 3144.600 kb on - strand, within BT2520 at 3144.600 kb on - strand, within BT2520 at 3144.600 kb on - strand, within BT2520 at 3144.604 kb on - strand, within BT2520 at 3144.604 kb on - strand, within BT2520 at 3144.687 kb on - strand, within BT2520 at 3144.727 kb on - strand, within BT2520 at 3144.727 kb on - strand, within BT2520 at 3144.791 kb on - strand, within BT2520 at 3144.803 kb on - strand, within BT2520 at 3144.889 kb on - strand at 3144.889 kb on - strand at 3144.896 kb on - strand at 3144.901 kb on - strand at 3144.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 3,143,529 - BT2518 0.64 +0.1 3,143,728 - -1.5 3,143,731 - -2.2 3,143,735 - -1.1 3,143,780 - +1.8 3,144,050 - BT2519 0.67 +1.5 3,144,060 - BT2519 0.70 -1.5 3,144,060 - BT2519 0.70 -0.2 3,144,098 - BT2519 0.80 -4.1 3,144,102 - BT2519 0.81 -0.5 3,144,102 - BT2519 0.81 -0.8 3,144,102 - BT2519 0.81 +0.3 3,144,102 - BT2519 0.81 -2.5 3,144,182 - -1.5 3,144,197 - -0.7 3,144,197 - -1.5 3,144,386 - +0.7 3,144,527 - BT2520 0.48 +0.1 3,144,548 - BT2520 0.51 +1.0 3,144,574 - BT2520 0.55 -2.2 3,144,600 - BT2520 0.59 -0.9 3,144,600 - -0.9 3,144,600 - BT2520 0.59 -0.7 3,144,600 - BT2520 0.59 +1.8 3,144,600 - BT2520 0.59 -2.7 3,144,604 - BT2520 0.59 -1.9 3,144,604 - BT2520 0.59 -1.2 3,144,687 - BT2520 0.72 +1.1 3,144,727 - BT2520 0.78 -1.9 3,144,727 - BT2520 0.78 -1.5 3,144,791 - BT2520 0.87 -1.1 3,144,803 - BT2520 0.89 -0.9 3,144,889 - -1.9 3,144,889 - -1.9 3,144,896 - +0.1 3,144,901 - +0.7 3,144,915 - -2.9
Or see this region's nucleotide sequence