Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2226 and BT2227 are separated by 68 nucleotides BT2227 and BT2228 are separated by 83 nucleotides BT2228 and BT2229 are separated by 43 nucleotides BT2229 and BT2230 are separated by 68 nucleotides
BT2226: BT2226 - transcriptional regulator (NCBI ptt file), at 2,795,082 to 2,795,927
BT2226
BT2227: BT2227 - conserved hypothetical protein (NCBI ptt file), at 2,795,996 to 2,796,463
BT2227
BT2228: BT2228 - hypothetical protein (NCBI ptt file), at 2,796,547 to 2,796,882
BT2228
BT2229: BT2229 - thioredoxin (thioredoxin M) (NCBI ptt file), at 2,796,926 to 2,797,240
BT2229
BT2230: BT2230 - DNA polymerase III alpha subunit (NCBI ptt file), at 2,797,309 to 2,801,112
BT2230
Position (kb)
2796
2797 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2795.574 kb on + strand, within BT2226 at 2795.574 kb on + strand, within BT2226 at 2795.576 kb on + strand, within BT2226 at 2795.577 kb on - strand, within BT2226 at 2795.577 kb on - strand, within BT2226 at 2795.578 kb on + strand, within BT2226 at 2795.579 kb on - strand, within BT2226 at 2795.673 kb on + strand, within BT2226 at 2795.689 kb on + strand, within BT2226 at 2795.706 kb on + strand, within BT2226 at 2795.707 kb on - strand, within BT2226 at 2795.707 kb on - strand, within BT2226 at 2795.707 kb on - strand, within BT2226 at 2795.754 kb on + strand, within BT2226 at 2795.754 kb on + strand, within BT2226 at 2795.797 kb on + strand, within BT2226 at 2795.799 kb on + strand, within BT2226 at 2795.799 kb on + strand, within BT2226 at 2795.810 kb on - strand, within BT2226 at 2795.850 kb on + strand at 2795.850 kb on + strand at 2795.850 kb on + strand at 2795.850 kb on + strand at 2795.856 kb on + strand at 2795.929 kb on - strand at 2796.030 kb on + strand at 2796.079 kb on - strand, within BT2227 at 2796.083 kb on - strand, within BT2227 at 2796.154 kb on - strand, within BT2227 at 2796.201 kb on - strand, within BT2227 at 2796.201 kb on - strand, within BT2227 at 2796.271 kb on - strand, within BT2227 at 2796.292 kb on - strand, within BT2227 at 2796.341 kb on + strand at 2796.342 kb on - strand, within BT2227 at 2796.382 kb on + strand, within BT2227 at 2796.384 kb on + strand, within BT2227 at 2796.385 kb on - strand, within BT2227 at 2796.461 kb on + strand at 2796.510 kb on - strand at 2796.576 kb on - strand at 2796.620 kb on + strand, within BT2228 at 2796.620 kb on + strand, within BT2228 at 2796.621 kb on - strand, within BT2228 at 2796.621 kb on - strand, within BT2228 at 2796.621 kb on - strand, within BT2228 at 2796.621 kb on - strand, within BT2228 at 2796.621 kb on - strand, within BT2228 at 2796.665 kb on - strand, within BT2228 at 2796.689 kb on - strand, within BT2228 at 2796.689 kb on - strand, within BT2228 at 2796.768 kb on - strand, within BT2228 at 2796.813 kb on - strand, within BT2228 at 2796.813 kb on - strand, within BT2228 at 2796.889 kb on - strand at 2796.893 kb on - strand at 2796.895 kb on + strand at 2797.263 kb on - strand at 2797.270 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 2,795,574 + BT2226 0.58 -1.5 2,795,574 + BT2226 0.58 +0.1 2,795,576 + BT2226 0.58 -3.1 2,795,577 - BT2226 0.59 -0.2 2,795,577 - BT2226 0.59 -1.6 2,795,578 + BT2226 0.59 -3.9 2,795,579 - BT2226 0.59 +1.1 2,795,673 + BT2226 0.70 -2.8 2,795,689 + BT2226 0.72 -1.5 2,795,706 + BT2226 0.74 -2.3 2,795,707 - BT2226 0.74 -2.0 2,795,707 - BT2226 0.74 -4.2 2,795,707 - BT2226 0.74 -1.5 2,795,754 + BT2226 0.79 -0.4 2,795,754 + BT2226 0.79 -3.3 2,795,797 + BT2226 0.85 -3.9 2,795,799 + BT2226 0.85 -0.6 2,795,799 + BT2226 0.85 -1.5 2,795,810 - BT2226 0.86 -0.9 2,795,850 + -0.9 2,795,850 + -0.9 2,795,850 + -1.5 2,795,850 + +1.5 2,795,856 + -1.5 2,795,929 - -0.9 2,796,030 + -0.1 2,796,079 - BT2227 0.18 +0.1 2,796,083 - BT2227 0.19 -2.9 2,796,154 - BT2227 0.34 -1.5 2,796,201 - BT2227 0.44 -1.4 2,796,201 - BT2227 0.44 -4.5 2,796,271 - BT2227 0.59 -1.2 2,796,292 - BT2227 0.63 +1.1 2,796,341 + -0.9 2,796,342 - BT2227 0.74 -0.8 2,796,382 + BT2227 0.82 -0.9 2,796,384 + BT2227 0.83 -4.3 2,796,385 - BT2227 0.83 -0.4 2,796,461 + -0.9 2,796,510 - -3.3 2,796,576 - -0.9 2,796,620 + BT2228 0.22 +1.5 2,796,620 + BT2228 0.22 -1.0 2,796,621 - BT2228 0.22 -1.9 2,796,621 - BT2228 0.22 +0.1 2,796,621 - BT2228 0.22 +1.6 2,796,621 - BT2228 0.22 -0.9 2,796,621 - BT2228 0.22 -2.5 2,796,665 - BT2228 0.35 -0.9 2,796,689 - BT2228 0.42 -2.3 2,796,689 - BT2228 0.42 -0.1 2,796,768 - BT2228 0.66 +0.1 2,796,813 - BT2228 0.79 -0.2 2,796,813 - BT2228 0.79 +0.3 2,796,889 - -2.3 2,796,893 - -1.5 2,796,895 + -1.5 2,797,263 - +0.1 2,797,270 - -1.5
Or see this region's nucleotide sequence