Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2121

Experiment: BHIS with Simvastatin 30 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2120 and BT2121 overlap by 20 nucleotidesBT2121 and BT2122 are separated by 105 nucleotides BT2120: BT2120 - transcriptional regulator (NCBI ptt file), at 2,668,778 to 2,669,644 BT2120 BT2121: BT2121 - putative Na+-driven multidrug efflux pump (NCBI ptt file), at 2,669,625 to 2,670,977 BT2121 BT2122: BT2122 - lysyl-tRNA synthetase (NCBI ptt file), at 2,671,083 to 2,672,813 BT2122 Position (kb) 2669 2670 2671Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2668.668 kb on + strandat 2668.798 kb on + strandat 2668.799 kb on - strandat 2668.807 kb on + strandat 2668.808 kb on - strandat 2668.809 kb on + strandat 2668.847 kb on - strandat 2668.851 kb on + strandat 2668.852 kb on - strandat 2668.855 kb on + strandat 2668.955 kb on + strand, within BT2120at 2668.955 kb on + strand, within BT2120at 2668.955 kb on + strand, within BT2120at 2669.029 kb on + strand, within BT2120at 2669.053 kb on + strand, within BT2120at 2669.058 kb on + strand, within BT2120at 2669.059 kb on - strand, within BT2120at 2669.059 kb on - strand, within BT2120at 2669.059 kb on - strand, within BT2120at 2669.081 kb on + strand, within BT2120at 2669.082 kb on - strand, within BT2120at 2669.124 kb on - strand, within BT2120at 2669.314 kb on + strand, within BT2120at 2669.355 kb on - strandat 2669.355 kb on - strand, within BT2120at 2669.355 kb on - strand, within BT2120at 2669.389 kb on + strand, within BT2120at 2669.476 kb on - strand, within BT2120at 2669.479 kb on + strand, within BT2120at 2669.480 kb on - strand, within BT2120at 2669.480 kb on - strand, within BT2120at 2669.480 kb on - strand, within BT2120at 2669.569 kb on + strandat 2669.585 kb on + strandat 2669.589 kb on + strandat 2669.590 kb on - strandat 2669.705 kb on + strandat 2669.705 kb on + strandat 2669.711 kb on + strandat 2669.738 kb on - strandat 2669.792 kb on + strand, within BT2121at 2669.793 kb on - strand, within BT2121at 2669.910 kb on - strand, within BT2121at 2669.990 kb on + strand, within BT2121at 2670.007 kb on + strand, within BT2121at 2670.065 kb on - strand, within BT2121at 2670.110 kb on + strand, within BT2121at 2670.188 kb on + strand, within BT2121at 2670.189 kb on - strand, within BT2121at 2670.192 kb on - strand, within BT2121at 2670.298 kb on + strand, within BT2121at 2670.394 kb on - strand, within BT2121at 2670.417 kb on - strand, within BT2121at 2670.432 kb on + strand, within BT2121at 2670.432 kb on + strand, within BT2121at 2670.437 kb on + strand, within BT2121at 2670.438 kb on - strand, within BT2121at 2670.438 kb on - strand, within BT2121at 2670.438 kb on - strand, within BT2121at 2670.440 kb on + strand, within BT2121at 2670.562 kb on + strand, within BT2121at 2670.670 kb on - strand, within BT2121at 2670.804 kb on - strand, within BT2121at 2670.804 kb on - strand, within BT2121at 2670.857 kb on + strandat 2670.861 kb on + strandat 2670.862 kb on - strandat 2670.871 kb on + strandat 2670.977 kb on + strandat 2670.978 kb on - strandat 2670.978 kb on - strandat 2670.982 kb on - strandat 2671.069 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM
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2,668,668 + +2.8
2,668,798 + -0.0
2,668,799 - -2.0
2,668,807 + -0.0
2,668,808 - -0.0
2,668,809 + -0.5
2,668,847 - -2.6
2,668,851 + -0.5
2,668,852 - +0.3
2,668,855 + +0.7
2,668,955 + BT2120 0.20 -1.0
2,668,955 + BT2120 0.20 -1.0
2,668,955 + BT2120 0.20 -0.0
2,669,029 + BT2120 0.29 -0.8
2,669,053 + BT2120 0.32 -1.9
2,669,058 + BT2120 0.32 +0.4
2,669,059 - BT2120 0.32 -1.6
2,669,059 - BT2120 0.32 -1.0
2,669,059 - BT2120 0.32 -3.4
2,669,081 + BT2120 0.35 -2.4
2,669,082 - BT2120 0.35 -1.8
2,669,124 - BT2120 0.40 -0.1
2,669,314 + BT2120 0.62 -2.0
2,669,355 - +1.6
2,669,355 - BT2120 0.67 -1.6
2,669,355 - BT2120 0.67 -1.0
2,669,389 + BT2120 0.70 -2.0
2,669,476 - BT2120 0.81 -2.5
2,669,479 + BT2120 0.81 -1.0
2,669,480 - BT2120 0.81 -2.4
2,669,480 - BT2120 0.81 +0.3
2,669,480 - BT2120 0.81 -2.5
2,669,569 + -3.6
2,669,585 + -0.3
2,669,589 + -2.4
2,669,590 - -2.6
2,669,705 + -3.8
2,669,705 + -0.0
2,669,711 + -1.6
2,669,738 - -1.0
2,669,792 + BT2121 0.12 -0.0
2,669,793 - BT2121 0.12 -2.0
2,669,910 - BT2121 0.21 -0.3
2,669,990 + BT2121 0.27 +0.3
2,670,007 + BT2121 0.28 -0.0
2,670,065 - BT2121 0.33 -0.4
2,670,110 + BT2121 0.36 -1.2
2,670,188 + BT2121 0.42 -0.6
2,670,189 - BT2121 0.42 -2.8
2,670,192 - BT2121 0.42 -4.2
2,670,298 + BT2121 0.50 -3.2
2,670,394 - BT2121 0.57 +0.3
2,670,417 - BT2121 0.59 +1.2
2,670,432 + BT2121 0.60 +3.1
2,670,432 + BT2121 0.60 +0.2
2,670,437 + BT2121 0.60 -2.0
2,670,438 - BT2121 0.60 -1.0
2,670,438 - BT2121 0.60 -1.0
2,670,438 - BT2121 0.60 -0.6
2,670,440 + BT2121 0.60 -3.5
2,670,562 + BT2121 0.69 -1.6
2,670,670 - BT2121 0.77 +0.4
2,670,804 - BT2121 0.87 +0.5
2,670,804 - BT2121 0.87 -1.0
2,670,857 + -1.0
2,670,861 + -1.6
2,670,862 - -1.0
2,670,871 + -0.5
2,670,977 + -1.9
2,670,978 - -2.8
2,670,978 - -1.6
2,670,982 - -4.3
2,671,069 + -2.3

Or see this region's nucleotide sequence