Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2120 and BT2121 overlap by 20 nucleotides BT2121 and BT2122 are separated by 105 nucleotides
BT2120: BT2120 - transcriptional regulator (NCBI ptt file), at 2,668,778 to 2,669,644
BT2120
BT2121: BT2121 - putative Na+-driven multidrug efflux pump (NCBI ptt file), at 2,669,625 to 2,670,977
BT2121
BT2122: BT2122 - lysyl-tRNA synthetase (NCBI ptt file), at 2,671,083 to 2,672,813
BT2122
Position (kb)
2669
2670
2671 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2668.668 kb on + strand at 2668.798 kb on + strand at 2668.799 kb on - strand at 2668.807 kb on + strand at 2668.808 kb on - strand at 2668.809 kb on + strand at 2668.847 kb on - strand at 2668.851 kb on + strand at 2668.852 kb on - strand at 2668.855 kb on + strand at 2668.955 kb on + strand, within BT2120 at 2668.955 kb on + strand, within BT2120 at 2668.955 kb on + strand, within BT2120 at 2669.029 kb on + strand, within BT2120 at 2669.053 kb on + strand, within BT2120 at 2669.058 kb on + strand, within BT2120 at 2669.059 kb on - strand, within BT2120 at 2669.059 kb on - strand, within BT2120 at 2669.059 kb on - strand, within BT2120 at 2669.081 kb on + strand, within BT2120 at 2669.082 kb on - strand, within BT2120 at 2669.124 kb on - strand, within BT2120 at 2669.314 kb on + strand, within BT2120 at 2669.355 kb on - strand at 2669.355 kb on - strand, within BT2120 at 2669.355 kb on - strand, within BT2120 at 2669.389 kb on + strand, within BT2120 at 2669.476 kb on - strand, within BT2120 at 2669.479 kb on + strand, within BT2120 at 2669.480 kb on - strand, within BT2120 at 2669.480 kb on - strand, within BT2120 at 2669.480 kb on - strand, within BT2120 at 2669.569 kb on + strand at 2669.585 kb on + strand at 2669.589 kb on + strand at 2669.590 kb on - strand at 2669.705 kb on + strand at 2669.705 kb on + strand at 2669.711 kb on + strand at 2669.738 kb on - strand at 2669.792 kb on + strand, within BT2121 at 2669.793 kb on - strand, within BT2121 at 2669.910 kb on - strand, within BT2121 at 2669.990 kb on + strand, within BT2121 at 2670.007 kb on + strand, within BT2121 at 2670.065 kb on - strand, within BT2121 at 2670.110 kb on + strand, within BT2121 at 2670.188 kb on + strand, within BT2121 at 2670.189 kb on - strand, within BT2121 at 2670.192 kb on - strand, within BT2121 at 2670.298 kb on + strand, within BT2121 at 2670.394 kb on - strand, within BT2121 at 2670.417 kb on - strand, within BT2121 at 2670.432 kb on + strand, within BT2121 at 2670.432 kb on + strand, within BT2121 at 2670.437 kb on + strand, within BT2121 at 2670.438 kb on - strand, within BT2121 at 2670.438 kb on - strand, within BT2121 at 2670.438 kb on - strand, within BT2121 at 2670.440 kb on + strand, within BT2121 at 2670.562 kb on + strand, within BT2121 at 2670.670 kb on - strand, within BT2121 at 2670.804 kb on - strand, within BT2121 at 2670.804 kb on - strand, within BT2121 at 2670.857 kb on + strand at 2670.861 kb on + strand at 2670.862 kb on - strand at 2670.871 kb on + strand at 2670.977 kb on + strand at 2670.978 kb on - strand at 2670.978 kb on - strand at 2670.982 kb on - strand at 2671.069 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 2,668,668 + +2.8 2,668,798 + -0.0 2,668,799 - -2.0 2,668,807 + -0.0 2,668,808 - -0.0 2,668,809 + -0.5 2,668,847 - -2.6 2,668,851 + -0.5 2,668,852 - +0.3 2,668,855 + +0.7 2,668,955 + BT2120 0.20 -1.0 2,668,955 + BT2120 0.20 -1.0 2,668,955 + BT2120 0.20 -0.0 2,669,029 + BT2120 0.29 -0.8 2,669,053 + BT2120 0.32 -1.9 2,669,058 + BT2120 0.32 +0.4 2,669,059 - BT2120 0.32 -1.6 2,669,059 - BT2120 0.32 -1.0 2,669,059 - BT2120 0.32 -3.4 2,669,081 + BT2120 0.35 -2.4 2,669,082 - BT2120 0.35 -1.8 2,669,124 - BT2120 0.40 -0.1 2,669,314 + BT2120 0.62 -2.0 2,669,355 - +1.6 2,669,355 - BT2120 0.67 -1.6 2,669,355 - BT2120 0.67 -1.0 2,669,389 + BT2120 0.70 -2.0 2,669,476 - BT2120 0.81 -2.5 2,669,479 + BT2120 0.81 -1.0 2,669,480 - BT2120 0.81 -2.4 2,669,480 - BT2120 0.81 +0.3 2,669,480 - BT2120 0.81 -2.5 2,669,569 + -3.6 2,669,585 + -0.3 2,669,589 + -2.4 2,669,590 - -2.6 2,669,705 + -3.8 2,669,705 + -0.0 2,669,711 + -1.6 2,669,738 - -1.0 2,669,792 + BT2121 0.12 -0.0 2,669,793 - BT2121 0.12 -2.0 2,669,910 - BT2121 0.21 -0.3 2,669,990 + BT2121 0.27 +0.3 2,670,007 + BT2121 0.28 -0.0 2,670,065 - BT2121 0.33 -0.4 2,670,110 + BT2121 0.36 -1.2 2,670,188 + BT2121 0.42 -0.6 2,670,189 - BT2121 0.42 -2.8 2,670,192 - BT2121 0.42 -4.2 2,670,298 + BT2121 0.50 -3.2 2,670,394 - BT2121 0.57 +0.3 2,670,417 - BT2121 0.59 +1.2 2,670,432 + BT2121 0.60 +3.1 2,670,432 + BT2121 0.60 +0.2 2,670,437 + BT2121 0.60 -2.0 2,670,438 - BT2121 0.60 -1.0 2,670,438 - BT2121 0.60 -1.0 2,670,438 - BT2121 0.60 -0.6 2,670,440 + BT2121 0.60 -3.5 2,670,562 + BT2121 0.69 -1.6 2,670,670 - BT2121 0.77 +0.4 2,670,804 - BT2121 0.87 +0.5 2,670,804 - BT2121 0.87 -1.0 2,670,857 + -1.0 2,670,861 + -1.6 2,670,862 - -1.0 2,670,871 + -0.5 2,670,977 + -1.9 2,670,978 - -2.8 2,670,978 - -1.6 2,670,982 - -4.3 2,671,069 + -2.3
Or see this region's nucleotide sequence