Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1700 and BT1701 are separated by 42 nucleotides BT1701 and BT1702 are separated by 236 nucleotides BT1702 and BT1703 are separated by 66 nucleotides
BT1700: BT1700 - conserved hypothetical protein (NCBI ptt file), at 2,098,455 to 2,099,654
BT1700
BT1701: BT1701 - hypothetical protein (NCBI ptt file), at 2,099,697 to 2,099,885
BT1701
BT1702: BT1702 - hypothetical protein (NCBI ptt file), at 2,100,122 to 2,100,748
BT1702
BT1703: BT1703 - hypothetical protein (NCBI ptt file), at 2,100,815 to 2,102,653
BT1703
Position (kb)
2099
2100 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2099.528 kb on + strand, within BT1700 at 2099.634 kb on + strand at 2099.720 kb on + strand, within BT1701 at 2099.720 kb on + strand, within BT1701 at 2099.786 kb on + strand, within BT1701 at 2099.786 kb on + strand, within BT1701 at 2099.812 kb on + strand, within BT1701 at 2099.869 kb on + strand at 2099.870 kb on - strand at 2099.870 kb on - strand at 2099.874 kb on - strand at 2099.910 kb on - strand at 2099.910 kb on - strand at 2100.000 kb on + strand at 2100.001 kb on - strand at 2100.031 kb on + strand at 2100.031 kb on + strand at 2100.031 kb on + strand at 2100.031 kb on + strand at 2100.096 kb on - strand at 2100.116 kb on + strand at 2100.243 kb on + strand, within BT1702 at 2100.244 kb on - strand, within BT1702 at 2100.245 kb on + strand, within BT1702 at 2100.310 kb on + strand, within BT1702 at 2100.312 kb on + strand, within BT1702 at 2100.313 kb on - strand, within BT1702 at 2100.342 kb on - strand at 2100.414 kb on + strand, within BT1702 at 2100.420 kb on + strand, within BT1702 at 2100.420 kb on + strand, within BT1702 at 2100.450 kb on + strand, within BT1702 at 2100.463 kb on + strand, within BT1702 at 2100.464 kb on - strand, within BT1702 at 2100.464 kb on - strand, within BT1702 at 2100.540 kb on + strand, within BT1702 at 2100.541 kb on - strand, within BT1702 at 2100.541 kb on - strand, within BT1702 at 2100.557 kb on + strand, within BT1702 at 2100.582 kb on + strand, within BT1702 at 2100.583 kb on - strand, within BT1702 at 2100.592 kb on + strand, within BT1702 at 2100.611 kb on + strand, within BT1702 at 2100.746 kb on + strand at 2100.753 kb on + strand at 2100.834 kb on + strand at 2100.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 2,099,528 + BT1700 0.89 -2.5 2,099,634 + -1.1 2,099,720 + BT1701 0.12 -1.2 2,099,720 + BT1701 0.12 +0.4 2,099,786 + BT1701 0.47 -0.2 2,099,786 + BT1701 0.47 -2.8 2,099,812 + BT1701 0.61 +1.0 2,099,869 + -4.2 2,099,870 - +1.4 2,099,870 - -2.4 2,099,874 - -2.2 2,099,910 - -0.2 2,099,910 - -0.2 2,100,000 + -0.2 2,100,001 - -2.2 2,100,031 + -2.8 2,100,031 + -1.2 2,100,031 + -1.2 2,100,031 + -2.0 2,100,096 - -2.5 2,100,116 + -0.2 2,100,243 + BT1702 0.19 -2.5 2,100,244 - BT1702 0.19 -0.4 2,100,245 + BT1702 0.20 -3.9 2,100,310 + BT1702 0.30 -2.2 2,100,312 + BT1702 0.30 -1.4 2,100,313 - BT1702 0.30 -2.2 2,100,342 - -1.2 2,100,414 + BT1702 0.47 -2.8 2,100,420 + BT1702 0.48 -2.2 2,100,420 + BT1702 0.48 -0.8 2,100,450 + BT1702 0.52 -1.8 2,100,463 + BT1702 0.54 -2.5 2,100,464 - BT1702 0.55 -2.4 2,100,464 - BT1702 0.55 +2.6 2,100,540 + BT1702 0.67 -2.2 2,100,541 - BT1702 0.67 -1.2 2,100,541 - BT1702 0.67 -2.0 2,100,557 + BT1702 0.69 -1.8 2,100,582 + BT1702 0.73 -1.8 2,100,583 - BT1702 0.74 +1.3 2,100,592 + BT1702 0.75 -0.2 2,100,611 + BT1702 0.78 -0.2 2,100,746 + -0.2 2,100,753 + -1.5 2,100,834 + -2.2 2,100,835 - -0.3
Or see this region's nucleotide sequence