Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1684 and BT1685 are separated by 173 nucleotides BT1685 and BT1686 are separated by 30 nucleotides BT1686 and BT1687 are separated by 31 nucleotides BT1687 and BT1688 are separated by 24 nucleotides
BT1684: BT1684 - conserved hypothetical protein (NCBI ptt file), at 2,079,309 to 2,080,307
BT1684
BT1685: BT1685 - lactoylglutathione lyase and related protein (NCBI ptt file), at 2,080,481 to 2,080,885
BT1685
BT1686: BT1686 - propionyl-CoA carboxylase beta chain (NCBI ptt file), at 2,080,916 to 2,082,469
BT1686
BT1687: BT1687 - hypothetical protein (NCBI ptt file), at 2,082,501 to 2,083,421
BT1687
BT1688: BT1688 - biotin carboxyl carrier protein (BCCP) (NCBI ptt file), at 2,083,446 to 2,083,880
BT1688
Position (kb)
2080
2081
2082
2083 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2079.959 kb on + strand, within BT1684 at 2079.960 kb on - strand, within BT1684 at 2079.960 kb on - strand, within BT1684 at 2079.996 kb on + strand, within BT1684 at 2080.083 kb on + strand, within BT1684 at 2080.084 kb on - strand, within BT1684 at 2080.084 kb on - strand, within BT1684 at 2080.114 kb on + strand, within BT1684 at 2080.175 kb on - strand, within BT1684 at 2080.175 kb on - strand, within BT1684 at 2080.175 kb on - strand, within BT1684 at 2080.175 kb on - strand, within BT1684 at 2080.241 kb on + strand at 2080.369 kb on - strand at 2080.369 kb on - strand at 2080.480 kb on + strand at 2080.719 kb on + strand, within BT1685 at 2080.743 kb on + strand, within BT1685 at 2083.431 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 2,079,959 + BT1684 0.65 +1.1 2,079,960 - BT1684 0.65 +1.9 2,079,960 - BT1684 0.65 +3.3 2,079,996 + BT1684 0.69 -3.4 2,080,083 + BT1684 0.77 +2.3 2,080,084 - BT1684 0.78 +3.5 2,080,084 - BT1684 0.78 -3.0 2,080,114 + BT1684 0.81 -1.8 2,080,175 - BT1684 0.87 +0.6 2,080,175 - BT1684 0.87 +1.2 2,080,175 - BT1684 0.87 -1.8 2,080,175 - BT1684 0.87 +0.9 2,080,241 + +2.1 2,080,369 - -1.8 2,080,369 - +0.1 2,080,480 + -1.2 2,080,719 + BT1685 0.59 -1.8 2,080,743 + BT1685 0.65 -0.2 2,083,431 + -1.8
Or see this region's nucleotide sequence