Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1534 and BT1535 are separated by 29 nucleotides BT1535 and BT1536 are separated by 28 nucleotides BT1536 and BT1537 are separated by 243 nucleotides
BT1534: BT1534 - hypothetical protein (NCBI ptt file), at 1,893,713 to 1,894,339
BT1534
BT1535: BT1535 - ABC transporter ATP-binding protein (NCBI ptt file), at 1,894,369 to 1,895,034
BT1535
BT1536: BT1536 - ABC transporter permease (NCBI ptt file), at 1,895,063 to 1,896,310
BT1536
BT1537: BT1537 - putative NADH dehydrogenase (NCBI ptt file), at 1,896,554 to 1,897,945
BT1537
Position (kb)
1895
1896
1897 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1894.098 kb on + strand, within BT1534 at 1894.180 kb on - strand, within BT1534 at 1894.244 kb on + strand, within BT1534 at 1894.292 kb on + strand at 1894.292 kb on + strand at 1894.292 kb on + strand at 1894.301 kb on + strand at 1894.319 kb on + strand at 1894.370 kb on + strand at 1894.370 kb on + strand at 1894.401 kb on + strand at 1894.471 kb on - strand, within BT1535 at 1894.525 kb on - strand, within BT1535 at 1894.580 kb on - strand, within BT1535 at 1894.632 kb on + strand, within BT1535 at 1894.951 kb on + strand, within BT1535 at 1895.003 kb on + strand at 1895.041 kb on - strand at 1895.041 kb on - strand at 1895.049 kb on - strand at 1895.104 kb on + strand at 1895.104 kb on + strand at 1895.344 kb on + strand, within BT1536 at 1895.479 kb on - strand, within BT1536 at 1895.479 kb on - strand, within BT1536 at 1895.580 kb on + strand, within BT1536 at 1895.585 kb on - strand, within BT1536 at 1895.720 kb on - strand, within BT1536 at 1895.720 kb on - strand, within BT1536 at 1895.763 kb on + strand, within BT1536 at 1895.764 kb on - strand, within BT1536 at 1895.820 kb on + strand, within BT1536 at 1895.876 kb on - strand, within BT1536 at 1896.050 kb on + strand, within BT1536 at 1896.205 kb on + strand at 1896.205 kb on + strand at 1896.206 kb on - strand at 1896.209 kb on + strand at 1896.314 kb on + strand at 1896.357 kb on + strand at 1896.357 kb on + strand at 1896.474 kb on - strand at 1896.474 kb on - strand at 1896.475 kb on + strand at 1896.475 kb on + strand at 1896.475 kb on + strand at 1896.476 kb on - strand at 1896.509 kb on - strand at 1896.561 kb on - strand at 1896.635 kb on - strand at 1896.684 kb on + strand at 1896.720 kb on + strand, within BT1537 at 1896.749 kb on + strand, within BT1537 at 1896.789 kb on - strand, within BT1537 at 1897.025 kb on + strand, within BT1537 at 1897.043 kb on + strand, within BT1537 at 1897.159 kb on + strand, within BT1537 at 1897.165 kb on + strand, within BT1537
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,894,098 + BT1534 0.61 -1.8 1,894,180 - BT1534 0.74 -1.0 1,894,244 + BT1534 0.85 -3.4 1,894,292 + -2.8 1,894,292 + -2.5 1,894,292 + -1.8 1,894,301 + -1.5 1,894,319 + -2.5 1,894,370 + -1.2 1,894,370 + -0.2 1,894,401 + -2.9 1,894,471 - BT1535 0.15 -2.5 1,894,525 - BT1535 0.23 -1.8 1,894,580 - BT1535 0.32 -0.7 1,894,632 + BT1535 0.39 -2.0 1,894,951 + BT1535 0.87 -2.2 1,895,003 + -1.0 1,895,041 - -1.2 1,895,041 - +1.1 1,895,049 - -1.8 1,895,104 + +0.8 1,895,104 + -2.0 1,895,344 + BT1536 0.23 +0.1 1,895,479 - BT1536 0.33 -3.0 1,895,479 - BT1536 0.33 +1.4 1,895,580 + BT1536 0.41 -0.2 1,895,585 - BT1536 0.42 -0.2 1,895,720 - BT1536 0.53 -2.2 1,895,720 - BT1536 0.53 +1.2 1,895,763 + BT1536 0.56 +1.0 1,895,764 - BT1536 0.56 -1.8 1,895,820 + BT1536 0.61 -0.2 1,895,876 - BT1536 0.65 +1.6 1,896,050 + BT1536 0.79 +0.4 1,896,205 + -1.2 1,896,205 + -0.5 1,896,206 - -2.5 1,896,209 + +0.8 1,896,314 + -0.2 1,896,357 + +1.8 1,896,357 + +1.3 1,896,474 - -2.8 1,896,474 - -1.2 1,896,475 + -0.9 1,896,475 + +0.8 1,896,475 + -2.8 1,896,476 - +0.6 1,896,509 - -2.8 1,896,561 - -1.8 1,896,635 - -1.2 1,896,684 + -2.8 1,896,720 + BT1537 0.12 -0.2 1,896,749 + BT1537 0.14 -1.8 1,896,789 - BT1537 0.17 -1.8 1,897,025 + BT1537 0.34 -0.9 1,897,043 + BT1537 0.35 -0.0 1,897,159 + BT1537 0.43 -0.2 1,897,165 + BT1537 0.44 -3.0
Or see this region's nucleotide sequence