Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1521

Experiment: BHIS with Simvastatin 30 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1518 and BT1519 are separated by 13 nucleotidesBT1519 and BT1520 are separated by 209 nucleotidesBT1520 and BT1521 are separated by 2 nucleotidesBT1521 and BT1522 are separated by 56 nucleotides BT1518: BT1518 - hypothetical protein (NCBI ptt file), at 1,874,996 to 1,875,301 BT1518 BT1519: BT1519 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 1,875,315 to 1,875,761 BT1519 BT1520: BT1520 - putative transcriptional regulator (NCBI ptt file), at 1,875,971 to 1,876,249 BT1520 BT1521: BT1521 - conserved hypothetical protein (NCBI ptt file), at 1,876,252 to 1,877,181 BT1521 BT1522: BT1522 - putative aureobasidin A resistance protein (NCBI ptt file), at 1,877,238 to 1,878,155 BT1522 Position (kb) 1876 1877 1878Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1875.269 kb on - strand, within BT1518at 1875.381 kb on + strand, within BT1519at 1875.458 kb on + strand, within BT1519at 1875.460 kb on + strand, within BT1519at 1875.460 kb on + strand, within BT1519at 1875.528 kb on - strand, within BT1519at 1875.617 kb on + strand, within BT1519at 1875.618 kb on - strand, within BT1519at 1875.797 kb on - strandat 1875.798 kb on + strandat 1875.854 kb on - strandat 1875.911 kb on + strandat 1875.915 kb on - strandat 1875.916 kb on + strandat 1875.934 kb on + strandat 1875.935 kb on - strandat 1875.946 kb on - strandat 1875.946 kb on - strandat 1876.021 kb on - strand, within BT1520at 1876.063 kb on + strand, within BT1520at 1876.137 kb on + strand, within BT1520at 1876.141 kb on + strand, within BT1520at 1876.156 kb on + strand, within BT1520at 1876.179 kb on + strand, within BT1520at 1876.261 kb on - strandat 1876.545 kb on - strand, within BT1521at 1876.609 kb on - strand, within BT1521at 1876.613 kb on - strand, within BT1521at 1876.722 kb on - strand, within BT1521at 1876.740 kb on + strand, within BT1521at 1876.740 kb on + strand, within BT1521at 1876.744 kb on + strand, within BT1521at 1876.744 kb on + strand, within BT1521at 1876.745 kb on - strand, within BT1521at 1876.798 kb on + strand, within BT1521at 1876.817 kb on + strand, within BT1521at 1876.817 kb on + strand, within BT1521at 1876.875 kb on - strand, within BT1521at 1876.909 kb on + strand, within BT1521at 1876.909 kb on + strand, within BT1521at 1876.910 kb on - strand, within BT1521at 1876.911 kb on + strand, within BT1521at 1876.999 kb on - strand, within BT1521at 1877.005 kb on - strand, within BT1521at 1877.007 kb on - strand, within BT1521at 1877.047 kb on + strand, within BT1521at 1877.047 kb on + strand, within BT1521at 1877.057 kb on + strand, within BT1521at 1877.070 kb on + strand, within BT1521at 1877.071 kb on - strand, within BT1521at 1877.218 kb on - strandat 1877.240 kb on - strandat 1877.244 kb on - strandat 1877.257 kb on + strandat 1877.258 kb on - strandat 1877.258 kb on - strandat 1877.361 kb on - strand, within BT1522at 1877.479 kb on + strand, within BT1522

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM
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1,875,269 - BT1518 0.89 -1.2
1,875,381 + BT1519 0.15 -2.8
1,875,458 + BT1519 0.32 -0.7
1,875,460 + BT1519 0.32 -0.2
1,875,460 + BT1519 0.32 -3.9
1,875,528 - BT1519 0.48 -1.2
1,875,617 + BT1519 0.68 -1.8
1,875,618 - BT1519 0.68 -0.3
1,875,797 - -0.7
1,875,798 + -0.6
1,875,854 - -1.6
1,875,911 + -0.2
1,875,915 - -0.7
1,875,916 + -2.2
1,875,934 + -1.6
1,875,935 - -0.1
1,875,946 - -1.9
1,875,946 - -0.4
1,876,021 - BT1520 0.18 -2.8
1,876,063 + BT1520 0.33 -3.1
1,876,137 + BT1520 0.59 -2.2
1,876,141 + BT1520 0.61 -0.2
1,876,156 + BT1520 0.66 -3.8
1,876,179 + BT1520 0.75 -2.2
1,876,261 - -0.1
1,876,545 - BT1521 0.32 -1.3
1,876,609 - BT1521 0.38 -0.2
1,876,613 - BT1521 0.39 -0.7
1,876,722 - BT1521 0.51 -1.8
1,876,740 + BT1521 0.52 +2.7
1,876,740 + BT1521 0.52 -3.2
1,876,744 + BT1521 0.53 +0.1
1,876,744 + BT1521 0.53 +1.4
1,876,745 - BT1521 0.53 -0.2
1,876,798 + BT1521 0.59 -1.8
1,876,817 + BT1521 0.61 -1.1
1,876,817 + BT1521 0.61 +0.1
1,876,875 - BT1521 0.67 -1.0
1,876,909 + BT1521 0.71 -1.2
1,876,909 + BT1521 0.71 +0.5
1,876,910 - BT1521 0.71 -3.4
1,876,911 + BT1521 0.71 -1.8
1,876,999 - BT1521 0.80 -0.5
1,877,005 - BT1521 0.81 -0.7
1,877,007 - BT1521 0.81 -1.0
1,877,047 + BT1521 0.85 +1.2
1,877,047 + BT1521 0.85 -1.6
1,877,057 + BT1521 0.87 -0.2
1,877,070 + BT1521 0.88 +1.2
1,877,071 - BT1521 0.88 -0.2
1,877,218 - -0.2
1,877,240 - -2.6
1,877,244 - -0.2
1,877,257 + -2.8
1,877,258 - -1.2
1,877,258 - -2.5
1,877,361 - BT1522 0.13 -0.2
1,877,479 + BT1522 0.26 +1.3

Or see this region's nucleotide sequence