Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1343 and BT1344 overlap by 4 nucleotides BT1344 and BT1345 overlap by 1 nucleotides BT1345 and BT1346 are separated by 17 nucleotides
BT1343: BT1343 - putative capsule biosynthesis protein (NCBI ptt file), at 1,668,653 to 1,669,720
BT1343
BT1344: BT1344 - putative glycosyltransferase (NCBI ptt file), at 1,669,717 to 1,670,820
BT1344
BT1345: BT1345 - glycosyltransferase (NCBI ptt file), at 1,670,820 to 1,671,713
BT1345
BT1346: BT1346 - capsule biosynthesis protein capA (NCBI ptt file), at 1,671,731 to 1,672,756
BT1346
Position (kb)
1669
1670
1671 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1668.721 kb on + strand at 1668.749 kb on + strand at 1668.848 kb on + strand, within BT1343 at 1668.851 kb on + strand, within BT1343 at 1669.083 kb on + strand, within BT1343 at 1669.083 kb on + strand, within BT1343 at 1669.093 kb on + strand, within BT1343 at 1669.112 kb on + strand, within BT1343 at 1669.162 kb on + strand, within BT1343 at 1669.182 kb on + strand, within BT1343 at 1669.226 kb on + strand, within BT1343 at 1669.234 kb on + strand, within BT1343 at 1669.255 kb on + strand, within BT1343 at 1669.257 kb on + strand, within BT1343 at 1669.273 kb on + strand, within BT1343 at 1669.296 kb on + strand, within BT1343 at 1669.299 kb on - strand, within BT1343 at 1669.304 kb on + strand, within BT1343 at 1669.304 kb on + strand, within BT1343 at 1669.388 kb on + strand, within BT1343 at 1669.410 kb on + strand, within BT1343 at 1669.410 kb on + strand, within BT1343 at 1669.420 kb on + strand, within BT1343 at 1669.442 kb on - strand, within BT1343 at 1669.487 kb on + strand, within BT1343 at 1669.487 kb on + strand, within BT1343 at 1669.577 kb on - strand, within BT1343 at 1669.611 kb on - strand, within BT1343 at 1669.670 kb on + strand at 1669.693 kb on + strand at 1669.707 kb on + strand at 1669.739 kb on + strand at 1669.739 kb on + strand at 1669.833 kb on + strand, within BT1344 at 1669.878 kb on + strand, within BT1344 at 1669.902 kb on + strand, within BT1344 at 1669.913 kb on + strand, within BT1344 at 1669.914 kb on - strand, within BT1344 at 1669.935 kb on + strand, within BT1344 at 1669.950 kb on + strand, within BT1344 at 1669.987 kb on - strand, within BT1344 at 1670.014 kb on + strand, within BT1344 at 1670.014 kb on + strand, within BT1344 at 1670.014 kb on + strand, within BT1344 at 1670.015 kb on - strand, within BT1344 at 1670.020 kb on + strand, within BT1344 at 1670.076 kb on + strand, within BT1344 at 1670.080 kb on + strand, within BT1344 at 1670.137 kb on + strand, within BT1344 at 1670.137 kb on + strand, within BT1344 at 1670.155 kb on - strand, within BT1344 at 1670.297 kb on + strand, within BT1344 at 1670.339 kb on - strand, within BT1344 at 1670.416 kb on + strand, within BT1344 at 1670.417 kb on - strand, within BT1344 at 1670.420 kb on + strand, within BT1344 at 1670.425 kb on - strand, within BT1344 at 1670.454 kb on + strand, within BT1344 at 1670.459 kb on - strand, within BT1344 at 1670.527 kb on + strand, within BT1344 at 1670.581 kb on + strand, within BT1344 at 1670.590 kb on + strand, within BT1344 at 1670.632 kb on + strand, within BT1344 at 1670.647 kb on + strand, within BT1344 at 1670.676 kb on + strand, within BT1344 at 1670.702 kb on - strand, within BT1344 at 1670.712 kb on + strand at 1670.722 kb on + strand at 1670.733 kb on + strand at 1670.826 kb on + strand at 1670.831 kb on + strand at 1670.844 kb on + strand at 1670.973 kb on + strand, within BT1345 at 1670.985 kb on + strand, within BT1345 at 1670.991 kb on + strand, within BT1345 at 1671.179 kb on + strand, within BT1345 at 1671.180 kb on + strand, within BT1345 at 1671.180 kb on + strand, within BT1345 at 1671.182 kb on + strand, within BT1345 at 1671.203 kb on - strand, within BT1345 at 1671.227 kb on + strand, within BT1345 at 1671.239 kb on - strand, within BT1345 at 1671.299 kb on + strand, within BT1345 at 1671.381 kb on - strand, within BT1345 at 1671.389 kb on + strand, within BT1345 at 1671.442 kb on - strand, within BT1345 at 1671.469 kb on - strand, within BT1345 at 1671.491 kb on + strand, within BT1345 at 1671.554 kb on + strand, within BT1345 at 1671.580 kb on - strand, within BT1345 at 1671.609 kb on - strand, within BT1345 at 1671.615 kb on + strand, within BT1345 at 1671.615 kb on + strand, within BT1345 at 1671.616 kb on - strand, within BT1345 at 1671.616 kb on - strand, within BT1345 at 1671.714 kb on + strand at 1671.714 kb on + strand at 1671.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,668,721 + -0.2 1,668,749 + -1.8 1,668,848 + BT1343 0.18 -1.8 1,668,851 + BT1343 0.19 -2.5 1,669,083 + BT1343 0.40 -0.2 1,669,083 + BT1343 0.40 +1.8 1,669,093 + BT1343 0.41 +1.4 1,669,112 + BT1343 0.43 -0.2 1,669,162 + BT1343 0.48 -2.2 1,669,182 + BT1343 0.50 -1.8 1,669,226 + BT1343 0.54 -0.5 1,669,234 + BT1343 0.54 +0.5 1,669,255 + BT1343 0.56 -1.2 1,669,257 + BT1343 0.57 +1.0 1,669,273 + BT1343 0.58 +1.1 1,669,296 + BT1343 0.60 -1.4 1,669,299 - BT1343 0.60 -2.2 1,669,304 + BT1343 0.61 -0.2 1,669,304 + BT1343 0.61 +3.5 1,669,388 + BT1343 0.69 -3.0 1,669,410 + BT1343 0.71 +1.4 1,669,410 + BT1343 0.71 +0.8 1,669,420 + BT1343 0.72 -2.8 1,669,442 - BT1343 0.74 -1.2 1,669,487 + BT1343 0.78 +1.2 1,669,487 + BT1343 0.78 +2.1 1,669,577 - BT1343 0.87 +0.4 1,669,611 - BT1343 0.90 -2.2 1,669,670 + -1.2 1,669,693 + +2.6 1,669,707 + -0.2 1,669,739 + +1.4 1,669,739 + +1.9 1,669,833 + BT1344 0.11 +1.8 1,669,878 + BT1344 0.15 -3.0 1,669,902 + BT1344 0.17 -3.0 1,669,913 + BT1344 0.18 -0.2 1,669,914 - BT1344 0.18 +1.2 1,669,935 + BT1344 0.20 -1.1 1,669,950 + BT1344 0.21 -1.2 1,669,987 - BT1344 0.24 -0.2 1,670,014 + BT1344 0.27 -2.2 1,670,014 + BT1344 0.27 +1.4 1,670,014 + BT1344 0.27 -2.2 1,670,015 - BT1344 0.27 +2.1 1,670,020 + BT1344 0.27 -2.5 1,670,076 + BT1344 0.33 -0.3 1,670,080 + BT1344 0.33 +3.1 1,670,137 + BT1344 0.38 -2.5 1,670,137 + BT1344 0.38 -2.5 1,670,155 - BT1344 0.40 +0.9 1,670,297 + BT1344 0.53 -3.2 1,670,339 - BT1344 0.56 -0.2 1,670,416 + BT1344 0.63 -0.6 1,670,417 - BT1344 0.63 -1.2 1,670,420 + BT1344 0.64 +0.5 1,670,425 - BT1344 0.64 -1.8 1,670,454 + BT1344 0.67 -0.2 1,670,459 - BT1344 0.67 -2.2 1,670,527 + BT1344 0.73 -1.2 1,670,581 + BT1344 0.78 +4.0 1,670,590 + BT1344 0.79 -0.2 1,670,632 + BT1344 0.83 +1.6 1,670,647 + BT1344 0.84 +1.7 1,670,676 + BT1344 0.87 -0.2 1,670,702 - BT1344 0.89 +1.0 1,670,712 + -2.5 1,670,722 + +1.4 1,670,733 + -1.0 1,670,826 + -1.8 1,670,831 + -1.2 1,670,844 + -0.2 1,670,973 + BT1345 0.17 -1.2 1,670,985 + BT1345 0.18 -1.0 1,670,991 + BT1345 0.19 -0.2 1,671,179 + BT1345 0.40 -0.2 1,671,180 + BT1345 0.40 -1.2 1,671,180 + BT1345 0.40 -0.2 1,671,182 + BT1345 0.40 +1.4 1,671,203 - BT1345 0.43 -0.2 1,671,227 + BT1345 0.46 +2.5 1,671,239 - BT1345 0.47 -1.8 1,671,299 + BT1345 0.54 +1.9 1,671,381 - BT1345 0.63 -2.5 1,671,389 + BT1345 0.64 -1.2 1,671,442 - BT1345 0.70 -1.2 1,671,469 - BT1345 0.73 -2.2 1,671,491 + BT1345 0.75 -0.2 1,671,554 + BT1345 0.82 +2.5 1,671,580 - BT1345 0.85 -1.8 1,671,609 - BT1345 0.88 +1.8 1,671,615 + BT1345 0.89 -0.2 1,671,615 + BT1345 0.89 +1.9 1,671,616 - BT1345 0.89 -0.2 1,671,616 - BT1345 0.89 -0.2 1,671,714 + +2.4 1,671,714 + +2.6 1,671,720 + +3.1
Or see this region's nucleotide sequence