Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1294 and BT1295 are separated by 205 nucleotides BT1295 and BT1296 are separated by 52 nucleotides BT1296 and BT1297 are separated by 8 nucleotides BT1297 and BT1298 are separated by 42 nucleotides
BT1294: BT1294 - glycogen [starch] synthase, glycosyltransferase (NCBI ptt file), at 1,613,195 to 1,614,856
BT1294
BT1295: BT1295 - V-type sodium ATP synthase subunit K (NCBI ptt file), at 1,615,062 to 1,615,523
BT1295
BT1296: BT1296 - V-type ATP synthase subunit I (NCBI ptt file), at 1,615,576 to 1,617,387
BT1296
BT1297: BT1297 - V-type ATP synthase subunit D (NCBI ptt file), at 1,617,396 to 1,618,001
BT1297
BT1298: BT1298 - V-type ATP synthase subunit B (NCBI ptt file), at 1,618,044 to 1,619,369
BT1298
Position (kb)
1615
1616
1617
1618 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1614.590 kb on + strand, within BT1294 at 1614.755 kb on - strand at 1614.800 kb on - strand at 1615.145 kb on + strand, within BT1295 at 1615.374 kb on + strand, within BT1295 at 1615.439 kb on + strand, within BT1295 at 1615.530 kb on - strand at 1615.751 kb on - strand at 1615.771 kb on - strand, within BT1296 at 1615.774 kb on + strand, within BT1296 at 1615.878 kb on + strand, within BT1296 at 1615.912 kb on - strand, within BT1296 at 1616.136 kb on + strand, within BT1296 at 1616.167 kb on - strand, within BT1296 at 1616.229 kb on - strand, within BT1296 at 1616.250 kb on + strand, within BT1296 at 1616.477 kb on + strand, within BT1296 at 1616.571 kb on - strand, within BT1296 at 1616.576 kb on - strand, within BT1296 at 1616.741 kb on - strand, within BT1296 at 1616.741 kb on - strand, within BT1296 at 1616.966 kb on + strand, within BT1296 at 1617.184 kb on + strand, within BT1296 at 1617.357 kb on + strand at 1617.577 kb on - strand, within BT1297 at 1618.017 kb on + strand at 1618.154 kb on + strand at 1618.226 kb on - strand, within BT1298 at 1618.291 kb on - strand, within BT1298
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,614,590 + BT1294 0.84 +0.6 1,614,755 - -1.2 1,614,800 - -1.2 1,615,145 + BT1295 0.18 -2.8 1,615,374 + BT1295 0.68 -1.2 1,615,439 + BT1295 0.82 -0.4 1,615,530 - -0.2 1,615,751 - +0.6 1,615,771 - BT1296 0.11 +4.4 1,615,774 + BT1296 0.11 +1.1 1,615,878 + BT1296 0.17 +1.2 1,615,912 - BT1296 0.19 -0.2 1,616,136 + BT1296 0.31 +0.5 1,616,167 - BT1296 0.33 +0.8 1,616,229 - BT1296 0.36 -0.2 1,616,250 + BT1296 0.37 -1.8 1,616,477 + BT1296 0.50 +0.3 1,616,571 - BT1296 0.55 -1.2 1,616,576 - BT1296 0.55 +0.3 1,616,741 - BT1296 0.64 -1.8 1,616,741 - BT1296 0.64 -0.2 1,616,966 + BT1296 0.77 -1.8 1,617,184 + BT1296 0.89 +0.4 1,617,357 + +0.7 1,617,577 - BT1297 0.30 -0.2 1,618,017 + -1.2 1,618,154 + -1.8 1,618,226 - BT1298 0.14 -1.2 1,618,291 - BT1298 0.19 -3.6
Or see this region's nucleotide sequence