Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0910 and BT0911 are separated by 239 nucleotides BT0911 and BT0912 are separated by 15 nucleotides BT0912 and BT0913 overlap by 8 nucleotides
BT0910: BT0910 - magnesium chelatase, subunit I (NCBI ptt file), at 1,121,037 to 1,122,032
BT0910
BT0911: BT0911 - putative integration host factor IHF alpha subunit (NCBI ptt file), at 1,122,272 to 1,123,735
BT0911
BT0912: BT0912 - DNA-binding protein HU (NCBI ptt file), at 1,123,751 to 1,124,023
BT0912
BT0913: BT0913 - putative Fe-S oxidoreductase (NCBI ptt file), at 1,124,016 to 1,125,326
BT0913
Position (kb)
1122
1123
1124 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1122.231 kb on + strand at 1122.241 kb on + strand at 1122.318 kb on - strand at 1122.318 kb on - strand at 1122.357 kb on - strand at 1122.361 kb on + strand at 1122.362 kb on - strand at 1122.364 kb on - strand at 1122.395 kb on + strand at 1122.436 kb on - strand, within BT0911 at 1122.590 kb on - strand, within BT0911 at 1122.590 kb on - strand, within BT0911 at 1122.644 kb on - strand, within BT0911 at 1122.703 kb on - strand, within BT0911 at 1122.716 kb on - strand, within BT0911 at 1122.735 kb on - strand, within BT0911 at 1122.807 kb on + strand, within BT0911 at 1122.808 kb on - strand, within BT0911 at 1122.828 kb on - strand, within BT0911 at 1122.862 kb on + strand, within BT0911 at 1122.890 kb on - strand, within BT0911 at 1122.922 kb on + strand, within BT0911 at 1122.980 kb on + strand, within BT0911 at 1123.295 kb on - strand, within BT0911 at 1123.457 kb on + strand, within BT0911 at 1123.457 kb on + strand, within BT0911 at 1123.462 kb on + strand, within BT0911 at 1123.541 kb on - strand, within BT0911 at 1123.560 kb on - strand, within BT0911 at 1123.745 kb on + strand at 1123.746 kb on - strand at 1123.750 kb on - strand at 1123.788 kb on + strand, within BT0912 at 1123.819 kb on + strand, within BT0912 at 1123.820 kb on - strand, within BT0912 at 1123.881 kb on - strand, within BT0912 at 1123.949 kb on + strand, within BT0912 at 1123.972 kb on + strand, within BT0912 at 1123.975 kb on + strand, within BT0912 at 1124.023 kb on + strand at 1124.268 kb on + strand, within BT0913 at 1124.269 kb on - strand, within BT0913 at 1124.269 kb on - strand, within BT0913 at 1124.332 kb on + strand, within BT0913 at 1124.336 kb on + strand, within BT0913 at 1124.506 kb on + strand, within BT0913 at 1124.507 kb on - strand, within BT0913 at 1124.584 kb on + strand, within BT0913 at 1124.694 kb on - strand, within BT0913 at 1124.728 kb on + strand, within BT0913
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,122,231 + -1.7 1,122,241 + -0.2 1,122,318 - +0.5 1,122,318 - -1.2 1,122,357 - -1.2 1,122,361 + +1.9 1,122,362 - -0.2 1,122,364 - -1.7 1,122,395 + +0.6 1,122,436 - BT0911 0.11 -0.5 1,122,590 - BT0911 0.22 +0.3 1,122,590 - BT0911 0.22 +0.8 1,122,644 - BT0911 0.25 -4.7 1,122,703 - BT0911 0.29 -0.2 1,122,716 - BT0911 0.30 +0.8 1,122,735 - BT0911 0.32 -0.2 1,122,807 + BT0911 0.37 -1.7 1,122,808 - BT0911 0.37 -1.2 1,122,828 - BT0911 0.38 -1.2 1,122,862 + BT0911 0.40 -0.2 1,122,890 - BT0911 0.42 -0.2 1,122,922 + BT0911 0.44 +0.1 1,122,980 + BT0911 0.48 -0.2 1,123,295 - BT0911 0.70 -1.7 1,123,457 + BT0911 0.81 -2.7 1,123,457 + BT0911 0.81 +0.7 1,123,462 + BT0911 0.81 -0.2 1,123,541 - BT0911 0.87 +0.4 1,123,560 - BT0911 0.88 -1.2 1,123,745 + -0.2 1,123,746 - -1.2 1,123,750 - +0.3 1,123,788 + BT0912 0.14 -1.2 1,123,819 + BT0912 0.25 -1.2 1,123,820 - BT0912 0.25 -1.2 1,123,881 - BT0912 0.48 -3.0 1,123,949 + BT0912 0.73 -1.2 1,123,972 + BT0912 0.81 -0.2 1,123,975 + BT0912 0.82 -0.2 1,124,023 + -2.5 1,124,268 + BT0913 0.19 -1.5 1,124,269 - BT0913 0.19 -1.2 1,124,269 - BT0913 0.19 -1.7 1,124,332 + BT0913 0.24 -1.7 1,124,336 + BT0913 0.24 -1.2 1,124,506 + BT0913 0.37 -2.5 1,124,507 - BT0913 0.37 -1.2 1,124,584 + BT0913 0.43 -1.2 1,124,694 - BT0913 0.52 -1.2 1,124,728 + BT0913 0.54 -0.2
Or see this region's nucleotide sequence