Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0353 and BT0354 are separated by 5 nucleotides BT0354 and BT0355 are separated by 21 nucleotides BT0355 and BT0356 are separated by 25 nucleotides
BT0353: BT0353 - putative sugar epimerase/aldolase (NCBI ptt file), at 425,643 to 426,326
BT0353
BT0354: BT0354 - conserved hypothetical protein (NCBI ptt file), at 426,332 to 427,009
BT0354
BT0355: BT0355 - L-arabinose/L-arabinobiose symporter (from data) , at 427,031 to 428,725
BT0355
BT0356: BT0356 - aldose 1-epimerase precursor (NCBI ptt file), at 428,751 to 429,890
BT0356
Position (kb)
427
428
429 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 426.031 kb on + strand, within BT0353 at 426.035 kb on + strand, within BT0353 at 426.084 kb on + strand, within BT0353 at 426.085 kb on - strand, within BT0353 at 426.101 kb on + strand, within BT0353 at 426.102 kb on - strand, within BT0353 at 426.177 kb on - strand, within BT0353 at 426.254 kb on + strand, within BT0353 at 426.326 kb on + strand at 426.693 kb on + strand, within BT0354 at 426.717 kb on + strand, within BT0354 at 427.047 kb on + strand at 427.151 kb on + strand at 427.220 kb on - strand, within BT0355 at 427.267 kb on - strand, within BT0355 at 427.467 kb on + strand, within BT0355 at 427.526 kb on + strand, within BT0355 at 427.612 kb on + strand, within BT0355 at 427.715 kb on - strand, within BT0355 at 427.850 kb on + strand, within BT0355 at 427.869 kb on + strand, within BT0355 at 427.931 kb on + strand, within BT0355 at 427.948 kb on + strand, within BT0355 at 427.948 kb on + strand, within BT0355 at 428.176 kb on + strand, within BT0355 at 428.176 kb on + strand, within BT0355 at 428.301 kb on - strand, within BT0355 at 428.343 kb on - strand, within BT0355 at 428.347 kb on - strand, within BT0355 at 428.351 kb on + strand, within BT0355 at 428.352 kb on - strand, within BT0355 at 428.352 kb on - strand, within BT0355 at 428.451 kb on - strand, within BT0355 at 428.669 kb on - strand at 428.672 kb on - strand at 428.702 kb on + strand at 428.914 kb on - strand, within BT0356 at 428.941 kb on + strand, within BT0356 at 428.969 kb on - strand, within BT0356 at 428.970 kb on + strand, within BT0356 at 429.220 kb on + strand, within BT0356 at 429.220 kb on + strand, within BT0356 at 429.311 kb on + strand, within BT0356 at 429.457 kb on + strand, within BT0356 at 429.460 kb on - strand, within BT0356 at 429.584 kb on + strand, within BT0356 at 429.606 kb on + strand, within BT0356 at 429.612 kb on - strand, within BT0356 at 429.614 kb on + strand, within BT0356 at 429.645 kb on + strand, within BT0356
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 426,031 + BT0353 0.57 -0.1 426,035 + BT0353 0.57 -0.1 426,084 + BT0353 0.64 -0.1 426,085 - BT0353 0.65 -1.1 426,101 + BT0353 0.67 -1.2 426,102 - BT0353 0.67 -0.6 426,177 - BT0353 0.78 -0.8 426,254 + BT0353 0.89 -0.1 426,326 + -0.1 426,693 + BT0354 0.53 +0.9 426,717 + BT0354 0.57 -2.9 427,047 + -0.1 427,151 + -0.7 427,220 - BT0355 0.11 -0.1 427,267 - BT0355 0.14 -1.1 427,467 + BT0355 0.26 -0.1 427,526 + BT0355 0.29 +2.2 427,612 + BT0355 0.34 -1.7 427,715 - BT0355 0.40 -1.1 427,850 + BT0355 0.48 +0.8 427,869 + BT0355 0.49 -0.1 427,931 + BT0355 0.53 -0.1 427,948 + BT0355 0.54 -1.1 427,948 + BT0355 0.54 -0.1 428,176 + BT0355 0.68 -0.3 428,176 + BT0355 0.68 +0.9 428,301 - BT0355 0.75 +1.2 428,343 - BT0355 0.77 -2.1 428,347 - BT0355 0.78 -0.1 428,351 + BT0355 0.78 -3.4 428,352 - BT0355 0.78 +2.2 428,352 - BT0355 0.78 -0.1 428,451 - BT0355 0.84 -0.4 428,669 - -2.1 428,672 - -3.2 428,702 + -2.4 428,914 - BT0356 0.14 -2.7 428,941 + BT0356 0.17 -0.1 428,969 - BT0356 0.19 -1.1 428,970 + BT0356 0.19 -2.1 429,220 + BT0356 0.41 -1.7 429,220 + BT0356 0.41 -0.1 429,311 + BT0356 0.49 +1.5 429,457 + BT0356 0.62 +2.2 429,460 - BT0356 0.62 -0.1 429,584 + BT0356 0.73 -3.1 429,606 + BT0356 0.75 -0.1 429,612 - BT0356 0.76 -1.1 429,614 + BT0356 0.76 -1.1 429,645 + BT0356 0.78 +1.7
Or see this region's nucleotide sequence