Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0336 and BT0337 are separated by 77 nucleotides BT0337 and BT0338 are separated by 769 nucleotides
BT0336: BT0336 - conserved hypothetical protein (NCBI ptt file), at 400,471 to 401,913
BT0336
BT0337: BT0337 - conserved hypothetical protein (NCBI ptt file), at 401,991 to 402,473
BT0337
BT0338: BT0338 - conserved hypothetical protein (NCBI ptt file), at 403,243 to 405,093
BT0338
Position (kb) 401
402
403 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 401.088 kb on + strand, within BT0336 at 401.163 kb on + strand, within BT0336 at 401.226 kb on - strand, within BT0336 at 401.230 kb on - strand, within BT0336 at 401.356 kb on + strand, within BT0336 at 401.388 kb on + strand, within BT0336 at 401.457 kb on - strand, within BT0336 at 401.800 kb on - strand at 401.822 kb on - strand at 401.875 kb on - strand at 401.879 kb on - strand at 401.893 kb on - strand at 401.967 kb on - strand at 401.996 kb on - strand at 401.999 kb on + strand at 402.130 kb on - strand, within BT0337 at 402.146 kb on + strand, within BT0337 at 402.217 kb on - strand, within BT0337 at 402.366 kb on - strand, within BT0337 at 402.641 kb on - strand at 402.732 kb on + strand at 402.748 kb on + strand at 402.827 kb on - strand at 402.956 kb on - strand at 402.956 kb on - strand at 402.959 kb on + strand at 402.960 kb on - strand at 402.991 kb on - strand at 403.097 kb on - strand at 403.125 kb on - strand at 403.223 kb on + strand at 403.224 kb on - strand at 403.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 401,088 + BT0336 0.43 -1.7 401,163 + BT0336 0.48 +1.5 401,226 - BT0336 0.52 -1.7 401,230 - BT0336 0.53 -0.1 401,356 + BT0336 0.61 -0.2 401,388 + BT0336 0.64 -0.8 401,457 - BT0336 0.68 -0.4 401,800 - -1.1 401,822 - -2.4 401,875 - +0.5 401,879 - -0.4 401,893 - -1.1 401,967 - -0.1 401,996 - -2.1 401,999 + -2.4 402,130 - BT0337 0.29 +0.0 402,146 + BT0337 0.32 -0.5 402,217 - BT0337 0.47 -1.7 402,366 - BT0337 0.78 -0.1 402,641 - -0.5 402,732 + +0.9 402,748 + -4.3 402,827 - -1.7 402,956 - -1.7 402,956 - -2.7 402,959 + -0.1 402,960 - +1.1 402,991 - -2.9 403,097 - +0.5 403,125 - -1.7 403,223 + -2.9 403,224 - -1.7 403,375 - +1.8
Or see this region's nucleotide sequence