Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0127 and BT0128 are separated by 183 nucleotides BT0128 and BT0129 are separated by 105 nucleotides
BT0127: BT0127 - putative transmembrane protein (NCBI ptt file), at 120,368 to 123,661
BT0127
BT0128: BT0128 - hypothetical protein (NCBI ptt file), at 123,845 to 124,243
BT0128
BT0129: BT0129 - mannonate dehydratase (NCBI ptt file), at 124,349 to 125,536
BT0129
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 122.926 kb on + strand, within BT0127 at 122.926 kb on + strand, within BT0127 at 123.054 kb on - strand, within BT0127 at 123.055 kb on + strand, within BT0127 at 123.107 kb on + strand, within BT0127 at 123.108 kb on - strand, within BT0127 at 123.136 kb on + strand, within BT0127 at 123.246 kb on - strand, within BT0127 at 123.254 kb on - strand, within BT0127 at 123.281 kb on + strand, within BT0127 at 123.360 kb on - strand at 123.363 kb on + strand at 123.437 kb on + strand at 123.556 kb on + strand at 123.677 kb on + strand at 123.678 kb on - strand at 123.882 kb on + strand at 123.934 kb on + strand, within BT0128 at 123.934 kb on + strand, within BT0128 at 123.934 kb on + strand, within BT0128 at 123.935 kb on - strand, within BT0128 at 123.955 kb on + strand, within BT0128 at 123.982 kb on + strand, within BT0128 at 123.983 kb on - strand, within BT0128 at 123.999 kb on + strand, within BT0128 at 124.021 kb on + strand, within BT0128 at 124.021 kb on + strand, within BT0128 at 124.130 kb on + strand, within BT0128 at 124.131 kb on - strand, within BT0128 at 124.132 kb on + strand, within BT0128 at 124.133 kb on - strand, within BT0128 at 124.186 kb on - strand, within BT0128 at 124.267 kb on - strand at 124.278 kb on - strand at 124.299 kb on + strand at 124.396 kb on + strand at 124.397 kb on - strand at 124.404 kb on - strand at 124.424 kb on - strand at 124.440 kb on + strand at 124.451 kb on - strand at 124.506 kb on - strand, within BT0129 at 124.506 kb on - strand, within BT0129 at 124.506 kb on - strand, within BT0129 at 124.549 kb on + strand, within BT0129 at 124.549 kb on + strand, within BT0129 at 124.549 kb on + strand, within BT0129 at 124.549 kb on + strand, within BT0129 at 124.616 kb on + strand, within BT0129 at 124.623 kb on - strand, within BT0129 at 124.640 kb on - strand, within BT0129 at 124.774 kb on + strand, within BT0129 at 124.825 kb on + strand, within BT0129 at 124.825 kb on + strand, within BT0129 at 124.836 kb on + strand, within BT0129 at 124.907 kb on - strand, within BT0129 at 125.151 kb on + strand, within BT0129 at 125.151 kb on + strand, within BT0129 at 125.154 kb on + strand, within BT0129 at 125.164 kb on + strand, within BT0129 at 125.184 kb on + strand, within BT0129 at 125.184 kb on + strand, within BT0129 at 125.184 kb on + strand, within BT0129 at 125.197 kb on + strand, within BT0129 at 125.197 kb on + strand, within BT0129 at 125.198 kb on - strand, within BT0129
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 122,926 + BT0127 0.78 -0.8 122,926 + BT0127 0.78 -0.2 123,054 - BT0127 0.82 -0.7 123,055 + BT0127 0.82 -2.8 123,107 + BT0127 0.83 -0.4 123,108 - BT0127 0.83 -3.0 123,136 + BT0127 0.84 -1.2 123,246 - BT0127 0.87 -2.2 123,254 - BT0127 0.88 -2.5 123,281 + BT0127 0.88 -1.2 123,360 - -1.8 123,363 + -1.0 123,437 + -1.2 123,556 + -1.5 123,677 + -1.2 123,678 - -0.2 123,882 + -2.0 123,934 + BT0128 0.22 -3.2 123,934 + BT0128 0.22 -2.8 123,934 + BT0128 0.22 -3.6 123,935 - BT0128 0.23 -2.5 123,955 + BT0128 0.28 -0.7 123,982 + BT0128 0.34 -1.2 123,983 - BT0128 0.35 -0.2 123,999 + BT0128 0.39 -1.8 124,021 + BT0128 0.44 -3.0 124,021 + BT0128 0.44 -2.8 124,130 + BT0128 0.71 -1.1 124,131 - BT0128 0.72 -0.2 124,132 + BT0128 0.72 -1.0 124,133 - BT0128 0.72 -1.2 124,186 - BT0128 0.85 -1.2 124,267 - -2.8 124,278 - -0.2 124,299 + -2.2 124,396 + +0.5 124,397 - -1.5 124,404 - -4.0 124,424 - -1.8 124,440 + -2.2 124,451 - -1.2 124,506 - BT0129 0.13 -2.2 124,506 - BT0129 0.13 -1.2 124,506 - BT0129 0.13 -0.2 124,549 + BT0129 0.17 -0.0 124,549 + BT0129 0.17 +1.0 124,549 + BT0129 0.17 -0.2 124,549 + BT0129 0.17 -0.8 124,616 + BT0129 0.22 -0.2 124,623 - BT0129 0.23 -0.2 124,640 - BT0129 0.24 -1.0 124,774 + BT0129 0.36 -0.2 124,825 + BT0129 0.40 -0.2 124,825 + BT0129 0.40 -1.8 124,836 + BT0129 0.41 -2.2 124,907 - BT0129 0.47 -0.5 125,151 + BT0129 0.68 -1.8 125,151 + BT0129 0.68 -0.9 125,154 + BT0129 0.68 -0.6 125,164 + BT0129 0.69 -1.8 125,184 + BT0129 0.70 -0.7 125,184 + BT0129 0.70 -2.8 125,184 + BT0129 0.70 -0.2 125,197 + BT0129 0.71 -0.8 125,197 + BT0129 0.71 -2.8 125,198 - BT0129 0.71 -0.2
Or see this region's nucleotide sequence