Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0056 and BT0057 overlap by 11 nucleotides BT0057 and BT0058 are separated by 71 nucleotides BT0058 and BT0059 overlap by 4 nucleotides BT0059 and BT0060 are separated by 38 nucleotides
BT0056: BT0056 - putative glycosyltransferase (NCBI ptt file), at 52,276 to 53,298
BT0056
BT0057: BT0057 - hypothetical protein (NCBI ptt file), at 53,288 to 53,575
BT0057
BT0058: BT0058 - hypothetical protein (NCBI ptt file), at 53,647 to 54,777
BT0058
BT0059: BT0059 - hypothetical protein (NCBI ptt file), at 54,774 to 55,715
BT0059
BT0060: BT0060 - putative polysaccharide export protein (NCBI ptt file), at 55,754 to 56,539
BT0060
Position (kb) 53
54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 52.721 kb on + strand, within BT0056 at 52.721 kb on + strand, within BT0056 at 52.721 kb on + strand, within BT0056 at 52.764 kb on - strand, within BT0056 at 52.794 kb on - strand, within BT0056 at 52.931 kb on - strand, within BT0056 at 52.931 kb on - strand, within BT0056 at 53.086 kb on + strand, within BT0056 at 53.114 kb on + strand, within BT0056 at 53.121 kb on + strand, within BT0056 at 53.121 kb on + strand, within BT0056 at 53.123 kb on + strand, within BT0056 at 53.124 kb on - strand, within BT0056 at 53.126 kb on - strand, within BT0056 at 53.150 kb on - strand, within BT0056 at 53.221 kb on - strand at 53.233 kb on - strand at 53.478 kb on - strand, within BT0057 at 53.478 kb on - strand, within BT0057 at 53.668 kb on - strand at 53.740 kb on - strand at 53.919 kb on - strand, within BT0058 at 53.963 kb on + strand, within BT0058 at 54.131 kb on - strand, within BT0058 at 54.437 kb on - strand, within BT0058 at 54.504 kb on + strand, within BT0058 at 54.551 kb on - strand, within BT0058 at 54.563 kb on - strand, within BT0058 at 55.037 kb on - strand, within BT0059 at 55.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 52,721 + BT0056 0.43 -0.2 52,721 + BT0056 0.43 -1.2 52,721 + BT0056 0.43 -2.3 52,764 - BT0056 0.48 -1.2 52,794 - BT0056 0.51 -0.2 52,931 - BT0056 0.64 -0.2 52,931 - BT0056 0.64 -1.2 53,086 + BT0056 0.79 -0.8 53,114 + BT0056 0.82 -0.0 53,121 + BT0056 0.83 +0.8 53,121 + BT0056 0.83 -0.2 53,123 + BT0056 0.83 -0.2 53,124 - BT0056 0.83 -1.2 53,126 - BT0056 0.83 -1.2 53,150 - BT0056 0.85 -2.0 53,221 - -1.8 53,233 - -1.2 53,478 - BT0057 0.66 -2.5 53,478 - BT0057 0.66 +1.6 53,668 - +0.3 53,740 - -2.8 53,919 - BT0058 0.24 -2.2 53,963 + BT0058 0.28 -1.8 54,131 - BT0058 0.43 -0.2 54,437 - BT0058 0.70 +1.2 54,504 + BT0058 0.76 +0.8 54,551 - BT0058 0.80 -2.2 54,563 - BT0058 0.81 -0.2 55,037 - BT0059 0.28 -0.8 55,735 - -3.2
Or see this region's nucleotide sequence