Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0041 and BT0042 overlap by 4 nucleotides BT0042 and BT0043 are separated by 30 nucleotides BT0043 and BT0044 are separated by 10 nucleotides
BT0041: BT0041 - F420H2:quinone oxidoreductase (NCBI ptt file), at 38,016 to 39,218
BT0041
BT0042: BT0042 - conserved hypothetical protein (NCBI ptt file), at 39,215 to 40,375
BT0042
BT0043: BT0043 - putative glycosyltransferase (NCBI ptt file), at 40,406 to 40,582
BT0043
BT0044: BT0044 - putative glycosyltransferase (NCBI ptt file), at 40,593 to 41,537
BT0044
Position (kb) 39
40
41 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 38.317 kb on + strand, within BT0041 at 38.366 kb on + strand, within BT0041 at 38.366 kb on + strand, within BT0041 at 38.414 kb on - strand, within BT0041 at 38.472 kb on - strand, within BT0041 at 38.528 kb on + strand, within BT0041 at 38.535 kb on + strand, within BT0041 at 38.656 kb on + strand, within BT0041 at 38.688 kb on - strand, within BT0041 at 38.690 kb on - strand, within BT0041 at 38.801 kb on + strand, within BT0041 at 38.804 kb on - strand, within BT0041 at 38.821 kb on - strand, within BT0041 at 38.823 kb on - strand, within BT0041 at 38.823 kb on - strand, within BT0041 at 38.903 kb on + strand, within BT0041 at 38.904 kb on - strand, within BT0041 at 38.982 kb on - strand, within BT0041 at 38.984 kb on - strand, within BT0041 at 38.984 kb on - strand, within BT0041 at 38.984 kb on - strand, within BT0041 at 39.010 kb on + strand, within BT0041 at 39.082 kb on - strand, within BT0041 at 39.215 kb on - strand at 39.223 kb on - strand at 39.255 kb on - strand at 39.255 kb on - strand at 39.264 kb on + strand at 39.264 kb on + strand at 39.319 kb on + strand at 39.335 kb on + strand, within BT0042 at 39.391 kb on + strand, within BT0042 at 39.401 kb on - strand, within BT0042 at 39.408 kb on + strand, within BT0042 at 39.408 kb on + strand, within BT0042 at 39.455 kb on + strand, within BT0042 at 39.456 kb on - strand, within BT0042 at 39.466 kb on + strand, within BT0042 at 39.480 kb on - strand, within BT0042 at 39.544 kb on + strand, within BT0042 at 39.564 kb on - strand, within BT0042 at 39.637 kb on + strand, within BT0042 at 39.695 kb on + strand, within BT0042 at 39.763 kb on - strand, within BT0042 at 39.813 kb on + strand, within BT0042 at 40.165 kb on - strand, within BT0042 at 40.232 kb on - strand, within BT0042 at 40.282 kb on + strand at 40.306 kb on - strand at 40.333 kb on + strand at 40.435 kb on + strand, within BT0043 at 40.436 kb on - strand, within BT0043 at 40.442 kb on - strand, within BT0043 at 40.468 kb on + strand, within BT0043 at 40.468 kb on + strand, within BT0043 at 40.584 kb on - strand at 40.592 kb on - strand at 40.613 kb on - strand at 40.713 kb on - strand, within BT0044 at 40.775 kb on - strand, within BT0044 at 40.830 kb on - strand, within BT0044 at 40.830 kb on - strand, within BT0044 at 41.045 kb on + strand, within BT0044 at 41.076 kb on - strand at 41.167 kb on + strand, within BT0044 at 41.191 kb on - strand, within BT0044 at 41.202 kb on - strand, within BT0044 at 41.247 kb on - strand, within BT0044
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 38,317 + BT0041 0.25 -2.2 38,366 + BT0041 0.29 -0.2 38,366 + BT0041 0.29 -1.8 38,414 - BT0041 0.33 -0.2 38,472 - BT0041 0.38 -2.6 38,528 + BT0041 0.43 -3.5 38,535 + BT0041 0.43 -3.4 38,656 + BT0041 0.53 -0.2 38,688 - BT0041 0.56 -3.5 38,690 - BT0041 0.56 -1.2 38,801 + BT0041 0.65 -0.5 38,804 - BT0041 0.66 -0.2 38,821 - BT0041 0.67 -2.2 38,823 - BT0041 0.67 -0.8 38,823 - BT0041 0.67 -0.2 38,903 + BT0041 0.74 -2.5 38,904 - BT0041 0.74 -1.2 38,982 - BT0041 0.80 -1.2 38,984 - BT0041 0.80 -0.2 38,984 - BT0041 0.80 -0.2 38,984 - BT0041 0.80 -0.2 39,010 + BT0041 0.83 -1.8 39,082 - BT0041 0.89 +0.5 39,215 - -1.8 39,223 - -1.8 39,255 - -0.7 39,255 - -1.5 39,264 + -3.9 39,264 + -0.2 39,319 + -2.2 39,335 + BT0042 0.10 -1.2 39,391 + BT0042 0.15 -3.4 39,401 - BT0042 0.16 -0.2 39,408 + BT0042 0.17 -3.0 39,408 + BT0042 0.17 -3.2 39,455 + BT0042 0.21 -1.8 39,456 - BT0042 0.21 -0.2 39,466 + BT0042 0.22 -3.1 39,480 - BT0042 0.23 -1.2 39,544 + BT0042 0.28 -2.8 39,564 - BT0042 0.30 -2.8 39,637 + BT0042 0.36 -0.2 39,695 + BT0042 0.41 -2.8 39,763 - BT0042 0.47 -2.4 39,813 + BT0042 0.52 -3.8 40,165 - BT0042 0.82 -1.2 40,232 - BT0042 0.88 -1.2 40,282 + -0.2 40,306 - -3.5 40,333 + -0.2 40,435 + BT0043 0.16 +0.6 40,436 - BT0043 0.17 -0.2 40,442 - BT0043 0.20 -1.2 40,468 + BT0043 0.35 -0.2 40,468 + BT0043 0.35 -3.7 40,584 - -1.6 40,592 - -1.2 40,613 - -1.2 40,713 - BT0044 0.13 -2.2 40,775 - BT0044 0.19 +0.8 40,830 - BT0044 0.25 -0.2 40,830 - BT0044 0.25 -0.2 41,045 + BT0044 0.48 -1.2 41,076 - -0.2 41,167 + BT0044 0.61 -1.2 41,191 - BT0044 0.63 -2.2 41,202 - BT0044 0.64 +2.1 41,247 - BT0044 0.69 -1.2
Or see this region's nucleotide sequence