Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20235 and BWI76_RS20240 are separated by 76 nucleotides BWI76_RS20240 and BWI76_RS20245 are separated by 46 nucleotides BWI76_RS20245 and BWI76_RS20250 overlap by 8 nucleotides
BWI76_RS20235: BWI76_RS20235 - acetate kinase, at 4,240,377 to 4,241,579
_RS20235
BWI76_RS20240: BWI76_RS20240 - phosphate acetyltransferase, at 4,241,656 to 4,243,800
_RS20240
BWI76_RS20245: BWI76_RS20245 - transketolase subunit B, at 4,243,847 to 4,244,800
_RS20245
BWI76_RS20250: BWI76_RS20250 - hypothetical protein, at 4,244,793 to 4,245,623
_RS20250
Position (kb)
4241
4242
4243
4244 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4240.747 kb on + strand, within BWI76_RS20235 at 4241.607 kb on + strand at 4241.964 kb on - strand, within BWI76_RS20240 at 4242.998 kb on + strand, within BWI76_RS20240 at 4243.710 kb on + strand at 4243.750 kb on + strand at 4243.928 kb on - strand at 4243.942 kb on + strand at 4243.976 kb on + strand, within BWI76_RS20245 at 4244.137 kb on - strand, within BWI76_RS20245 at 4244.455 kb on + strand, within BWI76_RS20245 at 4244.507 kb on - strand, within BWI76_RS20245 at 4244.668 kb on + strand, within BWI76_RS20245 at 4244.742 kb on + strand at 4244.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM remove 4,240,747 + BWI76_RS20235 0.31 -2.2 4,241,607 + -0.0 4,241,964 - BWI76_RS20240 0.14 -1.6 4,242,998 + BWI76_RS20240 0.63 +0.6 4,243,710 + +2.5 4,243,750 + -1.6 4,243,928 - +0.1 4,243,942 + -1.1 4,243,976 + BWI76_RS20245 0.14 +0.3 4,244,137 - BWI76_RS20245 0.30 -0.9 4,244,455 + BWI76_RS20245 0.64 +0.2 4,244,507 - BWI76_RS20245 0.69 -3.0 4,244,668 + BWI76_RS20245 0.86 -0.1 4,244,742 + -1.6 4,244,791 + +0.4
Or see this region's nucleotide sequence