Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13840

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13830 and BWI76_RS13835 are separated by 184 nucleotidesBWI76_RS13835 and BWI76_RS13840 are separated by 236 nucleotidesBWI76_RS13840 and BWI76_RS13845 are separated by 114 nucleotides BWI76_RS13830: BWI76_RS13830 - NAD-dependent malic enzyme, at 2,885,110 to 2,886,807 _RS13830 BWI76_RS13835: BWI76_RS13835 - 30S ribosomal protein S22, at 2,886,992 to 2,887,132 _RS13835 BWI76_RS13840: BWI76_RS13840 - MBL fold metallo-hydrolase, at 2,887,369 to 2,888,220 _RS13840 BWI76_RS13845: BWI76_RS13845 - LysR family transcriptional regulator, at 2,888,335 to 2,889,237 _RS13845 Position (kb) 2887 2888 2889Strain fitness (log2 ratio) -1 0 1 2at 2886.500 kb on - strand, within BWI76_RS13830at 2886.526 kb on + strand, within BWI76_RS13830at 2886.539 kb on + strand, within BWI76_RS13830at 2886.702 kb on + strandat 2886.715 kb on + strandat 2886.750 kb on + strandat 2886.760 kb on + strandat 2886.761 kb on + strandat 2886.877 kb on - strandat 2886.886 kb on + strandat 2886.886 kb on + strandat 2886.886 kb on + strandat 2886.894 kb on - strandat 2886.894 kb on - strandat 2886.909 kb on - strandat 2887.140 kb on + strandat 2887.226 kb on + strandat 2887.327 kb on - strandat 2887.425 kb on - strandat 2887.853 kb on + strand, within BWI76_RS13840at 2887.917 kb on - strand, within BWI76_RS13840at 2888.086 kb on - strand, within BWI76_RS13840at 2888.096 kb on - strand, within BWI76_RS13840at 2888.096 kb on - strand, within BWI76_RS13840at 2888.177 kb on + strandat 2888.177 kb on + strandat 2888.340 kb on - strandat 2888.441 kb on + strand, within BWI76_RS13845at 2888.468 kb on + strand, within BWI76_RS13845at 2888.608 kb on - strand, within BWI76_RS13845at 2888.681 kb on + strand, within BWI76_RS13845at 2888.820 kb on + strand, within BWI76_RS13845at 2888.820 kb on + strand, within BWI76_RS13845

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM
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2,886,500 - BWI76_RS13830 0.82 -0.7
2,886,526 + BWI76_RS13830 0.83 -1.1
2,886,539 + BWI76_RS13830 0.84 -1.6
2,886,702 + -0.2
2,886,715 + +0.2
2,886,750 + -1.7
2,886,760 + +1.2
2,886,761 + -0.2
2,886,877 - +0.9
2,886,886 + -0.5
2,886,886 + +0.6
2,886,886 + -1.7
2,886,894 - -0.0
2,886,894 - +0.4
2,886,909 - +0.3
2,887,140 + -1.0
2,887,226 + +1.3
2,887,327 - -0.2
2,887,425 - -0.5
2,887,853 + BWI76_RS13840 0.57 -1.3
2,887,917 - BWI76_RS13840 0.64 -1.0
2,888,086 - BWI76_RS13840 0.84 -0.3
2,888,096 - BWI76_RS13840 0.85 -0.0
2,888,096 - BWI76_RS13840 0.85 -0.3
2,888,177 + +1.0
2,888,177 + +0.4
2,888,340 - -0.2
2,888,441 + BWI76_RS13845 0.12 -0.1
2,888,468 + BWI76_RS13845 0.15 +0.5
2,888,608 - BWI76_RS13845 0.30 +1.9
2,888,681 + BWI76_RS13845 0.38 -1.5
2,888,820 + BWI76_RS13845 0.54 +0.3
2,888,820 + BWI76_RS13845 0.54 -0.9

Or see this region's nucleotide sequence