Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07295

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07290 and BWI76_RS07295 overlap by 4 nucleotidesBWI76_RS07295 and BWI76_RS07300 overlap by 4 nucleotidesBWI76_RS07300 and BWI76_RS07305 are separated by 146 nucleotides BWI76_RS07290: BWI76_RS07290 - PTS beta-glucoside transporter subunit EIIBCA, at 1,533,799 to 1,535,679 _RS07290 BWI76_RS07295: BWI76_RS07295 - 6-phospho-beta-glucosidase, at 1,535,676 to 1,537,100 _RS07295 BWI76_RS07300: BWI76_RS07300 - antitermination protein BlgG, at 1,537,097 to 1,537,936 _RS07300 BWI76_RS07305: BWI76_RS07305 - sucrose operon repressor, at 1,538,083 to 1,539,087 _RS07305 Position (kb) 1535 1536 1537 1538Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1534.934 kb on - strand, within BWI76_RS07290at 1535.111 kb on + strand, within BWI76_RS07290at 1535.111 kb on + strand, within BWI76_RS07290at 1535.111 kb on + strand, within BWI76_RS07290at 1535.119 kb on - strand, within BWI76_RS07290at 1535.119 kb on - strand, within BWI76_RS07290at 1535.119 kb on - strand, within BWI76_RS07290at 1535.343 kb on + strand, within BWI76_RS07290at 1535.392 kb on - strand, within BWI76_RS07290at 1535.408 kb on + strand, within BWI76_RS07290at 1535.416 kb on - strand, within BWI76_RS07290at 1535.449 kb on - strand, within BWI76_RS07290at 1535.552 kb on - strandat 1535.590 kb on + strandat 1535.600 kb on + strandat 1535.710 kb on + strandat 1535.738 kb on - strandat 1535.752 kb on - strandat 1535.864 kb on - strand, within BWI76_RS07295at 1535.888 kb on + strand, within BWI76_RS07295at 1536.067 kb on - strand, within BWI76_RS07295at 1536.102 kb on - strand, within BWI76_RS07295at 1536.109 kb on + strand, within BWI76_RS07295at 1536.284 kb on + strand, within BWI76_RS07295at 1536.373 kb on - strand, within BWI76_RS07295at 1536.423 kb on - strand, within BWI76_RS07295at 1536.664 kb on - strand, within BWI76_RS07295at 1536.788 kb on + strand, within BWI76_RS07295at 1536.811 kb on - strand, within BWI76_RS07295at 1536.941 kb on + strand, within BWI76_RS07295at 1536.949 kb on - strand, within BWI76_RS07295at 1537.184 kb on + strand, within BWI76_RS07300at 1537.280 kb on - strand, within BWI76_RS07300at 1537.280 kb on - strand, within BWI76_RS07300at 1537.377 kb on + strand, within BWI76_RS07300at 1537.588 kb on + strand, within BWI76_RS07300at 1537.658 kb on + strand, within BWI76_RS07300at 1537.665 kb on + strand, within BWI76_RS07300at 1537.732 kb on + strand, within BWI76_RS07300at 1537.863 kb on + strandat 1537.899 kb on + strandat 1537.949 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM
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1,534,934 - BWI76_RS07290 0.60 +1.3
1,535,111 + BWI76_RS07290 0.70 -0.1
1,535,111 + BWI76_RS07290 0.70 +0.2
1,535,111 + BWI76_RS07290 0.70 -0.8
1,535,119 - BWI76_RS07290 0.70 +0.7
1,535,119 - BWI76_RS07290 0.70 -0.4
1,535,119 - BWI76_RS07290 0.70 -0.3
1,535,343 + BWI76_RS07290 0.82 +1.0
1,535,392 - BWI76_RS07290 0.85 +0.8
1,535,408 + BWI76_RS07290 0.86 +0.9
1,535,416 - BWI76_RS07290 0.86 +0.3
1,535,449 - BWI76_RS07290 0.88 +0.6
1,535,552 - +1.3
1,535,590 + +0.5
1,535,600 + +0.2
1,535,710 + +0.2
1,535,738 - -0.2
1,535,752 - -2.8
1,535,864 - BWI76_RS07295 0.13 -0.1
1,535,888 + BWI76_RS07295 0.15 -0.0
1,536,067 - BWI76_RS07295 0.27 -0.3
1,536,102 - BWI76_RS07295 0.30 +0.3
1,536,109 + BWI76_RS07295 0.30 -1.5
1,536,284 + BWI76_RS07295 0.43 +0.9
1,536,373 - BWI76_RS07295 0.49 -0.7
1,536,423 - BWI76_RS07295 0.52 -2.7
1,536,664 - BWI76_RS07295 0.69 -3.7
1,536,788 + BWI76_RS07295 0.78 -2.2
1,536,811 - BWI76_RS07295 0.80 -1.4
1,536,941 + BWI76_RS07295 0.89 +0.8
1,536,949 - BWI76_RS07295 0.89 -0.6
1,537,184 + BWI76_RS07300 0.10 -0.4
1,537,280 - BWI76_RS07300 0.22 -1.1
1,537,280 - BWI76_RS07300 0.22 -0.2
1,537,377 + BWI76_RS07300 0.33 +0.2
1,537,588 + BWI76_RS07300 0.58 +0.2
1,537,658 + BWI76_RS07300 0.67 +0.1
1,537,665 + BWI76_RS07300 0.68 -0.7
1,537,732 + BWI76_RS07300 0.76 +0.3
1,537,863 + +0.6
1,537,899 + -1.4
1,537,949 - +2.5

Or see this region's nucleotide sequence