Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15225 and BPHYT_RS15230 are separated by 55 nucleotides BPHYT_RS15230 and BPHYT_RS15235 are separated by 101 nucleotides BPHYT_RS15235 and BPHYT_RS15240 are separated by 262 nucleotides
BPHYT_RS15225: BPHYT_RS15225 - fructose-1,6-bisphosphatase class 1, at 3,463,358 to 3,464,374
_RS15225
BPHYT_RS15230: BPHYT_RS15230 - tRNA-Thr, at 3,464,430 to 3,464,505
_RS15230
BPHYT_RS15235: BPHYT_RS15235 - hypothetical protein, at 3,464,607 to 3,464,813
_RS15235
BPHYT_RS15240: BPHYT_RS15240 - excinuclease ABC subunit A, at 3,465,076 to 3,467,760
_RS15240
Position (kb)
3464
3465 Strain fitness (log2 ratio)
-2
-1
0
1 at 3464.584 kb on + strand at 3464.585 kb on - strand at 3464.711 kb on + strand, within BPHYT_RS15235 at 3464.712 kb on - strand, within BPHYT_RS15235 at 3464.712 kb on - strand, within BPHYT_RS15235 at 3464.713 kb on + strand, within BPHYT_RS15235 at 3464.912 kb on + strand at 3464.939 kb on - strand at 3465.058 kb on + strand at 3465.268 kb on + strand at 3465.268 kb on + strand at 3465.456 kb on - strand, within BPHYT_RS15240 at 3465.467 kb on - strand, within BPHYT_RS15240 at 3465.578 kb on - strand, within BPHYT_RS15240 at 3465.589 kb on + strand, within BPHYT_RS15240 at 3465.700 kb on + strand, within BPHYT_RS15240 at 3465.700 kb on + strand, within BPHYT_RS15240 at 3465.788 kb on - strand, within BPHYT_RS15240
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB remove 3,464,584 + +0.3 3,464,585 - +0.5 3,464,711 + BPHYT_RS15235 0.50 -1.7 3,464,712 - BPHYT_RS15235 0.51 -0.1 3,464,712 - BPHYT_RS15235 0.51 +1.2 3,464,713 + BPHYT_RS15235 0.51 -0.9 3,464,912 + -0.1 3,464,939 - -0.4 3,465,058 + +0.7 3,465,268 + -0.9 3,465,268 + +0.7 3,465,456 - BPHYT_RS15240 0.14 -2.0 3,465,467 - BPHYT_RS15240 0.15 +0.0 3,465,578 - BPHYT_RS15240 0.19 +0.4 3,465,589 + BPHYT_RS15240 0.19 -0.4 3,465,700 + BPHYT_RS15240 0.23 +0.6 3,465,700 + BPHYT_RS15240 0.23 +0.0 3,465,788 - BPHYT_RS15240 0.27 -0.0
Or see this region's nucleotide sequence