Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12260 and BPHYT_RS12265 overlap by 14 nucleotides BPHYT_RS12265 and BPHYT_RS12270 overlap by 4 nucleotides BPHYT_RS12270 and BPHYT_RS12275 are separated by 560 nucleotides
BPHYT_RS12260: BPHYT_RS12260 - DNA polymerase, at 2,800,157 to 2,801,401
_RS12260
BPHYT_RS12265: BPHYT_RS12265 - alanine acetyltransferase, at 2,801,388 to 2,801,882
_RS12265
BPHYT_RS12270: BPHYT_RS12270 - O-sialoglycoprotein endopeptidase, at 2,801,879 to 2,802,709
_RS12270
BPHYT_RS12275: BPHYT_RS12275 - acyl-CoA-binding protein, at 2,803,270 to 2,803,539
_RS12275
Position (kb)
2801
2802
2803 Strain fitness (log2 ratio)
-2
-1
0
1 at 2801.516 kb on - strand, within BPHYT_RS12265 at 2801.744 kb on - strand, within BPHYT_RS12265 at 2801.853 kb on - strand at 2801.853 kb on - strand at 2801.855 kb on - strand at 2802.721 kb on + strand at 2803.086 kb on - strand at 2803.218 kb on + strand at 2803.218 kb on + strand at 2803.435 kb on + strand, within BPHYT_RS12275 at 2803.435 kb on + strand, within BPHYT_RS12275 at 2803.441 kb on + strand, within BPHYT_RS12275 at 2803.441 kb on + strand, within BPHYT_RS12275 at 2803.441 kb on + strand, within BPHYT_RS12275 at 2803.495 kb on + strand, within BPHYT_RS12275 at 2803.705 kb on - strand at 2803.707 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB remove 2,801,516 - BPHYT_RS12265 0.26 -0.8 2,801,744 - BPHYT_RS12265 0.72 -1.5 2,801,853 - +1.3 2,801,853 - -1.8 2,801,855 - -1.3 2,802,721 + +0.6 2,803,086 - -0.8 2,803,218 + +0.2 2,803,218 + -1.2 2,803,435 + BPHYT_RS12275 0.61 +0.1 2,803,435 + BPHYT_RS12275 0.61 +0.2 2,803,441 + BPHYT_RS12275 0.63 +1.5 2,803,441 + BPHYT_RS12275 0.63 -0.2 2,803,441 + BPHYT_RS12275 0.63 +0.3 2,803,495 + BPHYT_RS12275 0.83 +0.8 2,803,705 - +1.4 2,803,707 + -0.1
Or see this region's nucleotide sequence