Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12255 and BPHYT_RS12260 overlap by 4 nucleotides BPHYT_RS12260 and BPHYT_RS12265 overlap by 14 nucleotides BPHYT_RS12265 and BPHYT_RS12270 overlap by 4 nucleotides
BPHYT_RS12255: BPHYT_RS12255 - hypothetical protein, at 2,799,147 to 2,800,160
_RS12255
BPHYT_RS12260: BPHYT_RS12260 - DNA polymerase, at 2,800,157 to 2,801,401
_RS12260
BPHYT_RS12265: BPHYT_RS12265 - alanine acetyltransferase, at 2,801,388 to 2,801,882
_RS12265
BPHYT_RS12270: BPHYT_RS12270 - O-sialoglycoprotein endopeptidase, at 2,801,879 to 2,802,709
_RS12270
Position (kb)
2800
2801
2802 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2799.197 kb on + strand at 2799.198 kb on - strand at 2799.198 kb on - strand at 2799.219 kb on + strand at 2799.220 kb on - strand at 2799.220 kb on - strand at 2799.344 kb on + strand, within BPHYT_RS12255 at 2799.344 kb on + strand, within BPHYT_RS12255 at 2799.344 kb on + strand, within BPHYT_RS12255 at 2799.344 kb on + strand, within BPHYT_RS12255 at 2799.345 kb on - strand, within BPHYT_RS12255 at 2799.345 kb on - strand, within BPHYT_RS12255 at 2799.345 kb on - strand, within BPHYT_RS12255 at 2799.345 kb on - strand, within BPHYT_RS12255 at 2799.345 kb on - strand, within BPHYT_RS12255 at 2799.652 kb on + strand, within BPHYT_RS12255 at 2799.673 kb on - strand, within BPHYT_RS12255 at 2799.703 kb on + strand, within BPHYT_RS12255 at 2799.703 kb on + strand, within BPHYT_RS12255 at 2799.703 kb on + strand, within BPHYT_RS12255 at 2799.703 kb on + strand, within BPHYT_RS12255 at 2799.703 kb on + strand, within BPHYT_RS12255 at 2800.080 kb on - strand at 2801.516 kb on - strand, within BPHYT_RS12265 at 2801.744 kb on - strand, within BPHYT_RS12265 at 2801.853 kb on - strand at 2801.853 kb on - strand at 2801.855 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB remove 2,799,197 + +1.9 2,799,198 - -1.2 2,799,198 - +0.8 2,799,219 + -0.2 2,799,220 - -0.7 2,799,220 - -0.0 2,799,344 + BPHYT_RS12255 0.19 +0.0 2,799,344 + BPHYT_RS12255 0.19 +0.1 2,799,344 + BPHYT_RS12255 0.19 +0.1 2,799,344 + BPHYT_RS12255 0.19 +1.1 2,799,345 - BPHYT_RS12255 0.20 -2.5 2,799,345 - BPHYT_RS12255 0.20 +0.8 2,799,345 - BPHYT_RS12255 0.20 -0.7 2,799,345 - BPHYT_RS12255 0.20 -0.3 2,799,345 - BPHYT_RS12255 0.20 -0.5 2,799,652 + BPHYT_RS12255 0.50 -1.0 2,799,673 - BPHYT_RS12255 0.52 +0.4 2,799,703 + BPHYT_RS12255 0.55 +0.3 2,799,703 + BPHYT_RS12255 0.55 -2.2 2,799,703 + BPHYT_RS12255 0.55 +0.1 2,799,703 + BPHYT_RS12255 0.55 +0.4 2,799,703 + BPHYT_RS12255 0.55 -1.6 2,800,080 - -0.1 2,801,516 - BPHYT_RS12265 0.26 -0.8 2,801,744 - BPHYT_RS12265 0.72 -1.5 2,801,853 - +1.3 2,801,853 - -1.8 2,801,855 - -1.3
Or see this region's nucleotide sequence