Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1116

Experiment: Anaerobic 500 uM Zinc chloride sample2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1114 and Psest_1115 are separated by 21 nucleotidesPsest_1115 and Psest_1116 overlap by 8 nucleotidesPsest_1116 and Psest_1117 are separated by 84 nucleotidesPsest_1117 and Psest_1118 are separated by 57 nucleotides Psest_1114: Psest_1114 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 1,208,940 to 1,209,767 _1114 Psest_1115: Psest_1115 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 1,209,789 to 1,210,538 _1115 Psest_1116: Psest_1116 - glutamate racemase, at 1,210,531 to 1,211,325 _1116 Psest_1117: Psest_1117 - Lipid A 3-O-deacylase (PagL)., at 1,211,410 to 1,211,928 _1117 Psest_1118: Psest_1118 - Predicted Fe-S-cluster oxidoreductase, at 1,211,986 to 1,212,297 _1118 Position (kb) 1210 1211 1212Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1209.927 kb on + strand, within Psest_1115at 1209.927 kb on + strand, within Psest_1115at 1209.974 kb on + strand, within Psest_1115at 1210.340 kb on + strand, within Psest_1115at 1211.395 kb on + strandat 1211.395 kb on + strandat 1211.397 kb on + strandat 1211.397 kb on + strandat 1211.397 kb on + strandat 1211.398 kb on - strandat 1211.398 kb on - strandat 1211.617 kb on + strand, within Psest_1117at 1211.618 kb on - strand, within Psest_1117at 1211.677 kb on + strand, within Psest_1117at 1211.677 kb on + strand, within Psest_1117at 1211.678 kb on - strand, within Psest_1117at 1211.707 kb on + strand, within Psest_1117at 1211.707 kb on + strand, within Psest_1117at 1211.707 kb on + strand, within Psest_1117at 1211.745 kb on + strand, within Psest_1117at 1211.855 kb on - strand, within Psest_1117at 1211.926 kb on + strandat 1211.926 kb on + strandat 1211.926 kb on + strandat 1212.077 kb on + strand, within Psest_1118at 1212.078 kb on - strand, within Psest_1118at 1212.078 kb on - strand, within Psest_1118at 1212.315 kb on + strandat 1212.315 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Anaerobic 500 uM Zinc chloride sample2
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1,209,927 + Psest_1115 0.18 -3.7
1,209,927 + Psest_1115 0.18 -3.8
1,209,974 + Psest_1115 0.25 -3.3
1,210,340 + Psest_1115 0.73 -1.9
1,211,395 + -0.7
1,211,395 + -0.4
1,211,397 + -0.2
1,211,397 + +0.0
1,211,397 + -0.1
1,211,398 - +0.6
1,211,398 - -0.6
1,211,617 + Psest_1117 0.40 +0.1
1,211,618 - Psest_1117 0.40 +0.9
1,211,677 + Psest_1117 0.51 -0.0
1,211,677 + Psest_1117 0.51 +0.4
1,211,678 - Psest_1117 0.52 -0.9
1,211,707 + Psest_1117 0.57 +0.1
1,211,707 + Psest_1117 0.57 +0.3
1,211,707 + Psest_1117 0.57 +0.1
1,211,745 + Psest_1117 0.65 -0.0
1,211,855 - Psest_1117 0.86 -0.1
1,211,926 + +0.6
1,211,926 + -1.4
1,211,926 + +0.1
1,212,077 + Psest_1118 0.29 +0.7
1,212,078 - Psest_1118 0.29 +0.1
1,212,078 - Psest_1118 0.29 -0.3
1,212,315 + -0.4
1,212,315 + +0.1

Or see this region's nucleotide sequence