Experiment: Anaerobic 500 uM Zinc chloride sample1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1113 and Psest_1114 are separated by 42 nucleotides Psest_1114 and Psest_1115 are separated by 21 nucleotides Psest_1115 and Psest_1116 overlap by 8 nucleotides Psest_1116 and Psest_1117 are separated by 84 nucleotides
Psest_1113: Psest_1113 - peptide chain release factor 1, at 1,207,815 to 1,208,897
_1113
Psest_1114: Psest_1114 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 1,208,940 to 1,209,767
_1114
Psest_1115: Psest_1115 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 1,209,789 to 1,210,538
_1115
Psest_1116: Psest_1116 - glutamate racemase, at 1,210,531 to 1,211,325
_1116
Psest_1117: Psest_1117 - Lipid A 3-O-deacylase (PagL)., at 1,211,410 to 1,211,928
_1117
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1208.902 kb on + strand at 1208.902 kb on + strand at 1209.927 kb on + strand, within Psest_1115 at 1209.927 kb on + strand, within Psest_1115 at 1209.974 kb on + strand, within Psest_1115 at 1210.340 kb on + strand, within Psest_1115 at 1211.395 kb on + strand at 1211.395 kb on + strand at 1211.397 kb on + strand at 1211.397 kb on + strand at 1211.397 kb on + strand at 1211.398 kb on - strand at 1211.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic 500 uM Zinc chloride sample1 remove 1,208,902 + +0.5 1,208,902 + +0.3 1,209,927 + Psest_1115 0.18 -5.1 1,209,927 + Psest_1115 0.18 -2.6 1,209,974 + Psest_1115 0.25 -3.2 1,210,340 + Psest_1115 0.73 -1.7 1,211,395 + -0.3 1,211,395 + +0.1 1,211,397 + +0.1 1,211,397 + +0.5 1,211,397 + -0.0 1,211,398 - -0.4 1,211,398 - -0.1
Or see this region's nucleotide sequence