Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS12640 and LRK54_RS12645 are separated by 212 nucleotides LRK54_RS12645 and LRK54_RS12650 are separated by 132 nucleotides LRK54_RS12650 and LRK54_RS12655 are separated by 103 nucleotides
LRK54_RS12640: LRK54_RS12640 - UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase, at 2,821,815 to 2,823,188
_RS12640
LRK54_RS12645: LRK54_RS12645 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 2,823,401 to 2,823,514
_RS12645
LRK54_RS12650: LRK54_RS12650 - DUF1801 domain-containing protein, at 2,823,647 to 2,824,057
_RS12650
LRK54_RS12655: LRK54_RS12655 - M13 family metallopeptidase, at 2,824,161 to 2,826,212
_RS12655
Position (kb)
2823
2824
2825 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.247 kb on + strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.248 kb on - strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.379 kb on + strand at 2823.380 kb on - strand at 2823.380 kb on - strand at 2823.380 kb on - strand at 2823.380 kb on - strand at 2823.380 kb on - strand at 2823.380 kb on - strand at 2823.380 kb on - strand at 2823.537 kb on - strand at 2823.537 kb on - strand at 2823.537 kb on - strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.593 kb on + strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.594 kb on - strand at 2823.622 kb on - strand at 2823.691 kb on + strand, within LRK54_RS12650 at 2823.691 kb on + strand, within LRK54_RS12650 at 2823.691 kb on + strand, within LRK54_RS12650 at 2823.691 kb on + strand, within LRK54_RS12650 at 2823.691 kb on + strand, within LRK54_RS12650 at 2823.692 kb on - strand, within LRK54_RS12650 at 2823.692 kb on - strand, within LRK54_RS12650 at 2823.692 kb on - strand, within LRK54_RS12650 at 2823.692 kb on - strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.866 kb on + strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.867 kb on - strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.952 kb on + strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2823.953 kb on - strand, within LRK54_RS12650 at 2824.055 kb on + strand at 2824.055 kb on + strand at 2824.055 kb on + strand at 2824.055 kb on + strand at 2824.056 kb on - strand at 2824.056 kb on - strand at 2824.056 kb on - strand at 2824.056 kb on - strand at 2824.056 kb on - strand at 2824.056 kb on - strand at 2824.162 kb on + strand at 2824.162 kb on + strand at 2824.162 kb on + strand at 2824.163 kb on - strand at 2824.163 kb on - strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.195 kb on + strand at 2824.196 kb on - strand at 2824.196 kb on - strand at 2824.196 kb on - strand at 2824.196 kb on - strand at 2824.196 kb on - strand at 2824.196 kb on - strand at 2824.231 kb on + strand at 2824.232 kb on - strand at 2824.349 kb on - strand at 2824.423 kb on + strand, within LRK54_RS12655 at 2824.423 kb on + strand, within LRK54_RS12655 at 2824.423 kb on + strand, within LRK54_RS12655 at 2824.423 kb on + strand, within LRK54_RS12655 at 2824.423 kb on + strand, within LRK54_RS12655 at 2824.423 kb on + strand, within LRK54_RS12655 at 2824.424 kb on - strand, within LRK54_RS12655 at 2824.432 kb on + strand, within LRK54_RS12655 at 2824.432 kb on + strand, within LRK54_RS12655 at 2824.432 kb on + strand, within LRK54_RS12655 at 2824.432 kb on + strand, within LRK54_RS12655 at 2824.432 kb on + strand, within LRK54_RS12655 at 2824.433 kb on - strand, within LRK54_RS12655 at 2824.433 kb on - strand, within LRK54_RS12655 at 2824.433 kb on - strand, within LRK54_RS12655 at 2824.516 kb on + strand, within LRK54_RS12655 at 2824.677 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.678 kb on + strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.679 kb on - strand, within LRK54_RS12655 at 2824.847 kb on - strand, within LRK54_RS12655 at 2824.913 kb on - strand, within LRK54_RS12655 at 2824.913 kb on - strand, within LRK54_RS12655 at 2824.925 kb on + strand, within LRK54_RS12655 at 2824.925 kb on + strand, within LRK54_RS12655 at 2824.925 kb on + strand, within LRK54_RS12655 at 2824.926 kb on - strand, within LRK54_RS12655 at 2824.933 kb on + strand, within LRK54_RS12655 at 2825.010 kb on - strand, within LRK54_RS12655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,823,247 + +0.3 2,823,247 + -0.0 2,823,247 + -0.5 2,823,247 + -1.3 2,823,247 + -0.4 2,823,247 + +0.6 2,823,247 + +2.0 2,823,247 + -0.8 2,823,247 + -0.7 2,823,247 + +0.1 2,823,247 + -0.6 2,823,247 + +1.3 2,823,247 + +0.4 2,823,248 - -0.6 2,823,248 - -1.8 2,823,248 - +0.5 2,823,248 - +0.3 2,823,248 - +3.0 2,823,248 - -0.8 2,823,248 - -3.0 2,823,248 - -0.6 2,823,379 + +0.2 2,823,379 + +3.0 2,823,379 + +1.0 2,823,379 + -0.9 2,823,379 + -1.4 2,823,379 + -0.0 2,823,379 + -2.5 2,823,379 + +0.4 2,823,380 - -0.2 2,823,380 - -0.2 2,823,380 - -0.1 2,823,380 - -0.5 2,823,380 - -2.3 2,823,380 - -0.6 2,823,380 - -0.2 2,823,537 - -0.6 2,823,537 - +0.5 2,823,537 - +0.1 2,823,593 + -1.6 2,823,593 + +0.2 2,823,593 + -1.8 2,823,593 + -0.6 2,823,593 + -1.5 2,823,593 + -2.1 2,823,593 + -2.1 2,823,593 + -2.0 2,823,593 + -0.9 2,823,593 + +0.6 2,823,593 + -0.7 2,823,593 + -0.6 2,823,593 + +1.1 2,823,593 + +1.4 2,823,593 + +0.6 2,823,593 + +0.8 2,823,593 + -0.3 2,823,593 + +1.4 2,823,593 + +1.0 2,823,593 + -0.5 2,823,593 + +0.9 2,823,593 + +1.2 2,823,593 + -0.2 2,823,593 + -1.5 2,823,593 + -1.3 2,823,593 + -0.0 2,823,594 - +0.7 2,823,594 - +0.7 2,823,594 - +1.1 2,823,594 - -0.1 2,823,594 - +1.0 2,823,594 - +2.1 2,823,594 - -0.4 2,823,594 - +0.4 2,823,594 - +1.1 2,823,594 - -0.5 2,823,594 - -1.5 2,823,594 - -0.5 2,823,594 - +0.9 2,823,594 - +1.2 2,823,594 - +0.3 2,823,594 - +0.2 2,823,594 - -0.9 2,823,594 - +1.4 2,823,594 - +0.6 2,823,594 - -0.0 2,823,594 - +2.4 2,823,594 - -1.0 2,823,594 - -2.0 2,823,594 - -0.4 2,823,594 - +0.2 2,823,594 - +3.0 2,823,594 - +1.0 2,823,622 - +2.0 2,823,691 + LRK54_RS12650 0.11 -0.6 2,823,691 + LRK54_RS12650 0.11 -1.0 2,823,691 + LRK54_RS12650 0.11 -0.0 2,823,691 + LRK54_RS12650 0.11 -0.6 2,823,691 + LRK54_RS12650 0.11 +1.5 2,823,692 - LRK54_RS12650 0.11 -0.4 2,823,692 - LRK54_RS12650 0.11 -0.7 2,823,692 - LRK54_RS12650 0.11 +0.3 2,823,692 - LRK54_RS12650 0.11 -0.1 2,823,866 + LRK54_RS12650 0.53 -0.5 2,823,866 + LRK54_RS12650 0.53 +0.4 2,823,866 + LRK54_RS12650 0.53 -0.5 2,823,866 + LRK54_RS12650 0.53 -0.5 2,823,866 + LRK54_RS12650 0.53 -0.0 2,823,866 + LRK54_RS12650 0.53 +0.4 2,823,866 + LRK54_RS12650 0.53 -0.9 2,823,866 + LRK54_RS12650 0.53 -0.9 2,823,866 + LRK54_RS12650 0.53 +0.4 2,823,866 + LRK54_RS12650 0.53 -0.7 2,823,866 + LRK54_RS12650 0.53 +0.2 2,823,866 + LRK54_RS12650 0.53 -0.3 2,823,866 + LRK54_RS12650 0.53 +1.4 2,823,866 + LRK54_RS12650 0.53 +0.1 2,823,866 + LRK54_RS12650 0.53 -0.1 2,823,866 + LRK54_RS12650 0.53 -0.4 2,823,866 + LRK54_RS12650 0.53 +0.7 2,823,866 + LRK54_RS12650 0.53 -0.6 2,823,866 + LRK54_RS12650 0.53 +0.1 2,823,866 + LRK54_RS12650 0.53 -0.7 2,823,866 + LRK54_RS12650 0.53 +0.2 2,823,866 + LRK54_RS12650 0.53 -0.2 2,823,866 + LRK54_RS12650 0.53 -1.4 2,823,866 + LRK54_RS12650 0.53 +1.2 2,823,867 - LRK54_RS12650 0.54 -0.0 2,823,867 - LRK54_RS12650 0.54 +1.3 2,823,867 - LRK54_RS12650 0.54 -1.3 2,823,867 - LRK54_RS12650 0.54 -1.0 2,823,867 - LRK54_RS12650 0.54 -0.0 2,823,867 - LRK54_RS12650 0.54 +1.7 2,823,867 - LRK54_RS12650 0.54 +3.0 2,823,867 - LRK54_RS12650 0.54 -0.4 2,823,867 - LRK54_RS12650 0.54 +0.5 2,823,867 - LRK54_RS12650 0.54 -0.6 2,823,867 - LRK54_RS12650 0.54 -0.2 2,823,867 - LRK54_RS12650 0.54 +0.2 2,823,867 - LRK54_RS12650 0.54 -1.2 2,823,867 - LRK54_RS12650 0.54 -1.8 2,823,867 - LRK54_RS12650 0.54 +0.6 2,823,867 - LRK54_RS12650 0.54 -0.2 2,823,867 - LRK54_RS12650 0.54 -0.5 2,823,952 + LRK54_RS12650 0.74 -0.6 2,823,952 + LRK54_RS12650 0.74 +0.2 2,823,952 + LRK54_RS12650 0.74 -0.8 2,823,952 + LRK54_RS12650 0.74 +1.0 2,823,952 + LRK54_RS12650 0.74 -1.3 2,823,952 + LRK54_RS12650 0.74 +0.2 2,823,952 + LRK54_RS12650 0.74 +3.0 2,823,953 - LRK54_RS12650 0.74 +1.0 2,823,953 - LRK54_RS12650 0.74 -1.6 2,823,953 - LRK54_RS12650 0.74 -1.9 2,823,953 - LRK54_RS12650 0.74 -0.4 2,823,953 - LRK54_RS12650 0.74 +0.2 2,823,953 - LRK54_RS12650 0.74 -0.3 2,823,953 - LRK54_RS12650 0.74 +1.0 2,824,055 + -1.0 2,824,055 + +1.0 2,824,055 + -0.2 2,824,055 + -1.1 2,824,056 - +1.0 2,824,056 - +0.6 2,824,056 - -1.0 2,824,056 - -0.6 2,824,056 - -0.8 2,824,056 - -0.9 2,824,162 + -1.9 2,824,162 + -1.3 2,824,162 + +0.5 2,824,163 - -0.2 2,824,163 - -0.4 2,824,195 + +0.2 2,824,195 + -0.0 2,824,195 + -1.3 2,824,195 + -0.5 2,824,195 + -0.0 2,824,195 + -0.4 2,824,195 + +1.0 2,824,195 + -0.5 2,824,195 + -0.7 2,824,195 + -0.6 2,824,195 + -1.0 2,824,196 - -0.6 2,824,196 - +1.7 2,824,196 - +0.1 2,824,196 - +0.8 2,824,196 - +0.4 2,824,196 - -2.9 2,824,231 + -0.0 2,824,232 - -0.5 2,824,349 - +0.6 2,824,423 + LRK54_RS12655 0.13 -1.1 2,824,423 + LRK54_RS12655 0.13 -1.2 2,824,423 + LRK54_RS12655 0.13 -0.8 2,824,423 + LRK54_RS12655 0.13 -0.8 2,824,423 + LRK54_RS12655 0.13 +2.0 2,824,423 + LRK54_RS12655 0.13 +0.4 2,824,424 - LRK54_RS12655 0.13 -1.2 2,824,432 + LRK54_RS12655 0.13 -0.8 2,824,432 + LRK54_RS12655 0.13 +1.5 2,824,432 + LRK54_RS12655 0.13 +0.7 2,824,432 + LRK54_RS12655 0.13 -1.3 2,824,432 + LRK54_RS12655 0.13 +0.2 2,824,433 - LRK54_RS12655 0.13 -1.2 2,824,433 - LRK54_RS12655 0.13 +0.7 2,824,433 - LRK54_RS12655 0.13 +0.2 2,824,516 + LRK54_RS12655 0.17 +0.6 2,824,677 + LRK54_RS12655 0.25 +0.6 2,824,678 + LRK54_RS12655 0.25 +0.6 2,824,678 + LRK54_RS12655 0.25 -1.4 2,824,678 + LRK54_RS12655 0.25 +0.6 2,824,678 + LRK54_RS12655 0.25 -3.0 2,824,678 + LRK54_RS12655 0.25 -0.4 2,824,678 + LRK54_RS12655 0.25 -0.9 2,824,678 + LRK54_RS12655 0.25 -0.8 2,824,678 + LRK54_RS12655 0.25 +1.0 2,824,679 - LRK54_RS12655 0.25 -0.5 2,824,679 - LRK54_RS12655 0.25 -0.0 2,824,679 - LRK54_RS12655 0.25 -0.8 2,824,679 - LRK54_RS12655 0.25 -0.6 2,824,679 - LRK54_RS12655 0.25 -0.2 2,824,679 - LRK54_RS12655 0.25 -1.4 2,824,679 - LRK54_RS12655 0.25 -1.7 2,824,679 - LRK54_RS12655 0.25 +1.1 2,824,679 - LRK54_RS12655 0.25 -0.3 2,824,679 - LRK54_RS12655 0.25 -0.3 2,824,679 - LRK54_RS12655 0.25 -0.9 2,824,679 - LRK54_RS12655 0.25 +0.2 2,824,679 - LRK54_RS12655 0.25 +0.1 2,824,679 - LRK54_RS12655 0.25 -0.4 2,824,679 - LRK54_RS12655 0.25 +0.4 2,824,679 - LRK54_RS12655 0.25 -0.6 2,824,679 - LRK54_RS12655 0.25 -0.8 2,824,847 - LRK54_RS12655 0.33 +0.1 2,824,913 - LRK54_RS12655 0.37 -1.5 2,824,913 - LRK54_RS12655 0.37 +1.3 2,824,925 + LRK54_RS12655 0.37 +1.1 2,824,925 + LRK54_RS12655 0.37 -0.8 2,824,925 + LRK54_RS12655 0.37 +2.0 2,824,926 - LRK54_RS12655 0.37 +2.0 2,824,933 + LRK54_RS12655 0.38 -0.2 2,825,010 - LRK54_RS12655 0.41 +0.3
Or see this region's nucleotide sequence