Experiment: McCas_formate_complete nitrate 35.7142857142857 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08915 and MMP_RS06960 are separated by 27 nucleotides MMP_RS06960 and MMP_RS06965 are separated by 16 nucleotides MMP_RS06965 and MMP_RS06970 are separated by 6 nucleotides MMP_RS06970 and MMP_RS06975 are separated by 170 nucleotides
MMP_RS08915: MMP_RS08915 - 2 5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,327,842 to 1,328,924
_RS08915
MMP_RS06960: MMP_RS06960 - NAD+ synthase, at 1,328,952 to 1,329,728
_RS06960
MMP_RS06965: MMP_RS06965 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,329,745 to 1,331,058
_RS06965
MMP_RS06970: MMP_RS06970 - ribosomal protein S18-alanine N-acetyltransferase, at 1,331,065 to 1,331,550
_RS06970
MMP_RS06975: MMP_RS06975 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,331,721 to 1,332,509
_RS06975
Position (kb) 1329
1330
1331
1332 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1329.051 kb on + strand at 1329.290 kb on + strand at 1329.347 kb on + strand at 1329.349 kb on - strand at 1329.352 kb on + strand at 1329.546 kb on - strand at 1329.735 kb on + strand at 1329.768 kb on - strand at 1329.768 kb on - strand at 1329.768 kb on - strand at 1329.796 kb on + strand at 1329.849 kb on - strand at 1329.923 kb on + strand, within MMP_RS06965 at 1329.930 kb on + strand, within MMP_RS06965 at 1330.037 kb on + strand, within MMP_RS06965 at 1330.068 kb on - strand, within MMP_RS06965 at 1330.283 kb on + strand, within MMP_RS06965 at 1330.346 kb on + strand, within MMP_RS06965 at 1330.348 kb on + strand, within MMP_RS06965 at 1331.008 kb on - strand at 1331.008 kb on - strand at 1331.043 kb on + strand at 1331.063 kb on + strand at 1331.129 kb on + strand, within MMP_RS06970 at 1331.151 kb on + strand, within MMP_RS06970 at 1331.154 kb on + strand, within MMP_RS06970 at 1331.154 kb on + strand, within MMP_RS06970 at 1331.157 kb on - strand, within MMP_RS06970 at 1331.157 kb on - strand, within MMP_RS06970 at 1331.159 kb on - strand, within MMP_RS06970 at 1331.286 kb on - strand, within MMP_RS06970 at 1331.323 kb on - strand, within MMP_RS06970 at 1331.347 kb on + strand, within MMP_RS06970 at 1331.442 kb on + strand, within MMP_RS06970 at 1331.442 kb on + strand, within MMP_RS06970 at 1331.443 kb on - strand, within MMP_RS06970 at 1331.444 kb on + strand, within MMP_RS06970 at 1331.542 kb on - strand at 1331.551 kb on - strand at 1331.551 kb on - strand at 1331.553 kb on - strand at 1331.553 kb on - strand at 1331.554 kb on + strand at 1331.554 kb on + strand at 1331.554 kb on + strand at 1331.593 kb on + strand at 1331.598 kb on + strand at 1331.600 kb on + strand at 1331.666 kb on - strand at 1331.708 kb on + strand at 1331.776 kb on - strand at 1331.876 kb on - strand, within MMP_RS06975 at 1332.005 kb on + strand, within MMP_RS06975 at 1332.005 kb on + strand, within MMP_RS06975
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete nitrate 35.7142857142857 mM remove 1,329,051 + -0.7 1,329,290 + -2.4 1,329,347 + -1.2 1,329,349 - +0.1 1,329,352 + -1.7 1,329,546 - +0.3 1,329,735 + -2.4 1,329,768 - -1.3 1,329,768 - -1.3 1,329,768 - -1.1 1,329,796 + -0.9 1,329,849 - -0.8 1,329,923 + MMP_RS06965 0.14 -1.6 1,329,930 + MMP_RS06965 0.14 +0.4 1,330,037 + MMP_RS06965 0.22 -0.9 1,330,068 - MMP_RS06965 0.25 -1.9 1,330,283 + MMP_RS06965 0.41 -0.8 1,330,346 + MMP_RS06965 0.46 -1.5 1,330,348 + MMP_RS06965 0.46 -1.5 1,331,008 - -0.2 1,331,008 - -1.5 1,331,043 + -1.1 1,331,063 + -3.8 1,331,129 + MMP_RS06970 0.13 -1.7 1,331,151 + MMP_RS06970 0.18 -1.0 1,331,154 + MMP_RS06970 0.18 -2.1 1,331,154 + MMP_RS06970 0.18 +0.0 1,331,157 - MMP_RS06970 0.19 +3.1 1,331,157 - MMP_RS06970 0.19 +3.0 1,331,159 - MMP_RS06970 0.19 +1.1 1,331,286 - MMP_RS06970 0.45 +1.9 1,331,323 - MMP_RS06970 0.53 -0.1 1,331,347 + MMP_RS06970 0.58 +0.1 1,331,442 + MMP_RS06970 0.78 -0.6 1,331,442 + MMP_RS06970 0.78 -1.6 1,331,443 - MMP_RS06970 0.78 -0.9 1,331,444 + MMP_RS06970 0.78 -1.4 1,331,542 - +2.3 1,331,551 - +0.8 1,331,551 - +1.9 1,331,553 - +2.5 1,331,553 - +1.3 1,331,554 + -1.3 1,331,554 + +0.0 1,331,554 + -2.1 1,331,593 + -1.7 1,331,598 + +2.1 1,331,600 + -0.1 1,331,666 - -0.4 1,331,708 + +1.8 1,331,776 - +0.6 1,331,876 - MMP_RS06975 0.20 +1.7 1,332,005 + MMP_RS06975 0.36 +2.2 1,332,005 + MMP_RS06975 0.36 +0.4
Or see this region's nucleotide sequence