Experiment: McCas_formate_complete nitrate 35.7142857142857 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05630 and MMP_RS05635 are separated by 30 nucleotides MMP_RS05635 and MMP_RS05640 are separated by 124 nucleotides
MMP_RS05630: MMP_RS05630 - flippase, at 1,081,000 to 1,082,502
_RS05630
MMP_RS05635: MMP_RS05635 - NAD-dependent epimerase/dehydratase family protein, at 1,082,533 to 1,083,411
_RS05635
MMP_RS05640: MMP_RS05640 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,083,536 to 1,084,384
_RS05640
Position (kb)
1082
1083
1084 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 1081.631 kb on + strand at 1081.663 kb on - strand at 1081.865 kb on + strand at 1081.895 kb on + strand at 1081.921 kb on + strand at 1081.958 kb on - strand at 1081.990 kb on - strand at 1082.085 kb on + strand at 1082.155 kb on - strand at 1082.160 kb on + strand at 1082.281 kb on - strand at 1082.331 kb on - strand at 1082.349 kb on - strand at 1082.427 kb on + strand at 1082.495 kb on - strand at 1082.534 kb on - strand at 1082.545 kb on + strand at 1082.590 kb on + strand at 1082.595 kb on - strand at 1082.785 kb on + strand, within MMP_RS05635 at 1082.859 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.956 kb on + strand, within MMP_RS05635 at 1082.972 kb on - strand, within MMP_RS05635 at 1082.972 kb on - strand, within MMP_RS05635 at 1083.024 kb on - strand, within MMP_RS05635 at 1083.088 kb on - strand, within MMP_RS05635 at 1083.108 kb on + strand, within MMP_RS05635 at 1083.217 kb on - strand, within MMP_RS05635 at 1083.309 kb on - strand, within MMP_RS05635 at 1083.314 kb on + strand, within MMP_RS05635 at 1083.352 kb on - strand at 1083.357 kb on - strand at 1083.358 kb on + strand at 1083.358 kb on + strand at 1083.397 kb on - strand at 1083.431 kb on - strand at 1083.431 kb on - strand at 1083.435 kb on + strand at 1083.435 kb on + strand at 1083.438 kb on - strand at 1083.438 kb on - strand at 1083.439 kb on + strand at 1083.439 kb on + strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.459 kb on + strand at 1083.459 kb on + strand at 1083.469 kb on - strand at 1083.469 kb on - strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.489 kb on + strand at 1083.489 kb on + strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.497 kb on - strand at 1083.497 kb on - strand at 1083.532 kb on - strand at 1083.561 kb on - strand at 1083.623 kb on + strand, within MMP_RS05640 at 1083.708 kb on + strand, within MMP_RS05640 at 1083.773 kb on - strand, within MMP_RS05640 at 1083.774 kb on + strand, within MMP_RS05640 at 1083.778 kb on + strand, within MMP_RS05640 at 1083.816 kb on + strand, within MMP_RS05640 at 1083.816 kb on + strand, within MMP_RS05640 at 1083.892 kb on - strand, within MMP_RS05640 at 1084.008 kb on + strand, within MMP_RS05640 at 1084.087 kb on - strand, within MMP_RS05640 at 1084.088 kb on + strand, within MMP_RS05640 at 1084.089 kb on - strand, within MMP_RS05640 at 1084.092 kb on - strand, within MMP_RS05640 at 1084.222 kb on - strand, within MMP_RS05640 at 1084.235 kb on + strand, within MMP_RS05640 at 1084.268 kb on - strand, within MMP_RS05640 at 1084.269 kb on + strand, within MMP_RS05640 at 1084.272 kb on - strand, within MMP_RS05640 at 1084.273 kb on + strand, within MMP_RS05640 at 1084.278 kb on + strand, within MMP_RS05640 at 1084.389 kb on - strand at 1084.405 kb on - strand at 1084.405 kb on - strand at 1084.411 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete nitrate 35.7142857142857 mM remove 1,081,631 + +2.0 1,081,663 - -1.2 1,081,865 + -0.2 1,081,895 + +0.5 1,081,921 + -0.0 1,081,958 - -0.0 1,081,990 - +2.6 1,082,085 + -1.4 1,082,155 - -0.9 1,082,160 + -1.0 1,082,281 - -1.0 1,082,331 - -1.6 1,082,349 - +0.8 1,082,427 + -0.9 1,082,495 - -1.4 1,082,534 - +2.3 1,082,545 + +0.5 1,082,590 + +0.0 1,082,595 - -0.2 1,082,785 + MMP_RS05635 0.29 -3.6 1,082,859 - MMP_RS05635 0.37 -1.5 1,082,948 - MMP_RS05635 0.47 +0.1 1,082,948 - MMP_RS05635 0.47 -2.9 1,082,948 - MMP_RS05635 0.47 -2.3 1,082,956 + MMP_RS05635 0.48 -0.9 1,082,972 - MMP_RS05635 0.50 -1.1 1,082,972 - MMP_RS05635 0.50 -0.3 1,083,024 - MMP_RS05635 0.56 +0.9 1,083,088 - MMP_RS05635 0.63 -1.1 1,083,108 + MMP_RS05635 0.65 -0.6 1,083,217 - MMP_RS05635 0.78 +0.8 1,083,309 - MMP_RS05635 0.88 -1.9 1,083,314 + MMP_RS05635 0.89 -0.1 1,083,352 - -1.1 1,083,357 - -1.8 1,083,358 + -0.1 1,083,358 + +0.5 1,083,397 - +1.2 1,083,431 - +1.1 1,083,431 - -1.1 1,083,435 + +1.1 1,083,435 + +1.5 1,083,438 - +0.2 1,083,438 - +2.2 1,083,439 + +0.2 1,083,439 + -0.7 1,083,458 - +1.1 1,083,458 - +0.9 1,083,458 - +1.7 1,083,459 + -1.2 1,083,459 + +1.1 1,083,469 - +0.3 1,083,469 - +0.4 1,083,472 + +0.3 1,083,472 + -0.1 1,083,472 + +3.3 1,083,489 + +0.5 1,083,489 + -0.5 1,083,490 - -1.0 1,083,490 - +1.4 1,083,490 - +0.9 1,083,491 + +1.1 1,083,491 + +2.2 1,083,491 + -1.2 1,083,491 + +1.8 1,083,497 - +2.9 1,083,497 - -0.4 1,083,532 - -2.7 1,083,561 - +6.1 1,083,623 + MMP_RS05640 0.10 +0.7 1,083,708 + MMP_RS05640 0.20 +0.7 1,083,773 - MMP_RS05640 0.28 +4.9 1,083,774 + MMP_RS05640 0.28 +2.3 1,083,778 + MMP_RS05640 0.29 +5.0 1,083,816 + MMP_RS05640 0.33 +4.0 1,083,816 + MMP_RS05640 0.33 +5.4 1,083,892 - MMP_RS05640 0.42 -0.5 1,084,008 + MMP_RS05640 0.56 -1.8 1,084,087 - MMP_RS05640 0.65 -2.1 1,084,088 + MMP_RS05640 0.65 +4.2 1,084,089 - MMP_RS05640 0.65 +3.3 1,084,092 - MMP_RS05640 0.65 +7.2 1,084,222 - MMP_RS05640 0.81 -0.2 1,084,235 + MMP_RS05640 0.82 -0.3 1,084,268 - MMP_RS05640 0.86 +5.2 1,084,269 + MMP_RS05640 0.86 -0.9 1,084,272 - MMP_RS05640 0.87 -0.6 1,084,273 + MMP_RS05640 0.87 -1.0 1,084,278 + MMP_RS05640 0.87 +3.4 1,084,389 - +0.3 1,084,405 - +4.7 1,084,405 - +3.8 1,084,411 + +4.1
Or see this region's nucleotide sequence