Experiment: McCas_formate_complete nitrate 35.7142857142857 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05265 and MMP_RS05270 are separated by 67 nucleotides MMP_RS05270 and MMP_RS05275 are separated by 5 nucleotides
MMP_RS05265: MMP_RS05265 - 2-isopropylmalate synthase, at 1,005,952 to 1,007,430
_RS05265
MMP_RS05270: MMP_RS05270 - PEGA domain-containing protein, at 1,007,498 to 1,008,295
_RS05270
MMP_RS05275: MMP_RS05275 - AI-2E family transporter, at 1,008,301 to 1,009,266
_RS05275
Position (kb)
1007
1008
1009 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1006.673 kb on + strand, within MMP_RS05265 at 1006.735 kb on - strand, within MMP_RS05265 at 1007.221 kb on + strand, within MMP_RS05265 at 1007.478 kb on - strand at 1007.479 kb on + strand at 1007.479 kb on + strand at 1007.479 kb on + strand at 1007.479 kb on + strand at 1007.479 kb on + strand at 1007.480 kb on - strand at 1007.480 kb on - strand at 1007.480 kb on - strand at 1007.481 kb on + strand at 1007.481 kb on + strand at 1007.493 kb on - strand at 1007.533 kb on - strand at 1007.533 kb on - strand at 1007.533 kb on - strand at 1007.667 kb on + strand, within MMP_RS05270 at 1007.668 kb on - strand, within MMP_RS05270 at 1007.668 kb on - strand, within MMP_RS05270 at 1007.669 kb on + strand, within MMP_RS05270 at 1007.671 kb on - strand, within MMP_RS05270 at 1007.702 kb on + strand, within MMP_RS05270 at 1007.705 kb on + strand, within MMP_RS05270 at 1007.706 kb on - strand, within MMP_RS05270 at 1007.757 kb on - strand, within MMP_RS05270 at 1007.896 kb on - strand, within MMP_RS05270 at 1007.897 kb on + strand, within MMP_RS05270 at 1007.901 kb on + strand, within MMP_RS05270 at 1007.905 kb on + strand, within MMP_RS05270 at 1007.907 kb on - strand, within MMP_RS05270 at 1007.928 kb on + strand, within MMP_RS05270 at 1007.932 kb on - strand, within MMP_RS05270 at 1007.933 kb on + strand, within MMP_RS05270 at 1007.934 kb on - strand, within MMP_RS05270 at 1007.961 kb on - strand, within MMP_RS05270 at 1007.961 kb on - strand, within MMP_RS05270 at 1007.961 kb on - strand, within MMP_RS05270 at 1007.962 kb on + strand, within MMP_RS05270 at 1007.962 kb on + strand, within MMP_RS05270 at 1007.963 kb on - strand, within MMP_RS05270 at 1007.963 kb on - strand, within MMP_RS05270 at 1007.963 kb on - strand, within MMP_RS05270 at 1007.964 kb on + strand, within MMP_RS05270 at 1007.979 kb on + strand, within MMP_RS05270 at 1008.079 kb on + strand, within MMP_RS05270 at 1008.096 kb on + strand, within MMP_RS05270 at 1008.148 kb on - strand, within MMP_RS05270 at 1008.150 kb on - strand, within MMP_RS05270 at 1008.155 kb on + strand, within MMP_RS05270 at 1008.167 kb on + strand, within MMP_RS05270 at 1008.217 kb on - strand at 1008.259 kb on + strand at 1008.302 kb on - strand at 1008.347 kb on - strand at 1008.560 kb on - strand, within MMP_RS05275 at 1008.561 kb on + strand, within MMP_RS05275 at 1008.565 kb on - strand, within MMP_RS05275 at 1008.565 kb on - strand, within MMP_RS05275 at 1008.565 kb on - strand, within MMP_RS05275 at 1008.581 kb on - strand, within MMP_RS05275 at 1008.582 kb on + strand, within MMP_RS05275 at 1008.582 kb on + strand, within MMP_RS05275 at 1008.582 kb on + strand, within MMP_RS05275 at 1008.620 kb on + strand, within MMP_RS05275 at 1008.642 kb on - strand, within MMP_RS05275 at 1008.771 kb on - strand, within MMP_RS05275 at 1008.797 kb on - strand, within MMP_RS05275 at 1008.810 kb on - strand, within MMP_RS05275 at 1008.979 kb on - strand, within MMP_RS05275 at 1009.029 kb on - strand, within MMP_RS05275 at 1009.052 kb on + strand, within MMP_RS05275 at 1009.060 kb on - strand, within MMP_RS05275 at 1009.093 kb on - strand, within MMP_RS05275 at 1009.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete nitrate 35.7142857142857 mM remove 1,006,673 + MMP_RS05265 0.49 -0.3 1,006,735 - MMP_RS05265 0.53 +2.3 1,007,221 + MMP_RS05265 0.86 -0.7 1,007,478 - -1.7 1,007,479 + -1.3 1,007,479 + -0.9 1,007,479 + +0.7 1,007,479 + +0.8 1,007,479 + -1.8 1,007,480 - +1.1 1,007,480 - -1.3 1,007,480 - +0.2 1,007,481 + -0.1 1,007,481 + -1.2 1,007,493 - +0.4 1,007,533 - -1.1 1,007,533 - -0.3 1,007,533 - -1.7 1,007,667 + MMP_RS05270 0.21 -2.2 1,007,668 - MMP_RS05270 0.21 -0.5 1,007,668 - MMP_RS05270 0.21 -0.0 1,007,669 + MMP_RS05270 0.21 -0.9 1,007,671 - MMP_RS05270 0.22 -1.3 1,007,702 + MMP_RS05270 0.26 -0.6 1,007,705 + MMP_RS05270 0.26 -0.8 1,007,706 - MMP_RS05270 0.26 -1.7 1,007,757 - MMP_RS05270 0.32 -0.3 1,007,896 - MMP_RS05270 0.50 +0.7 1,007,897 + MMP_RS05270 0.50 -0.3 1,007,901 + MMP_RS05270 0.51 +1.4 1,007,905 + MMP_RS05270 0.51 -0.4 1,007,907 - MMP_RS05270 0.51 -3.5 1,007,928 + MMP_RS05270 0.54 -1.2 1,007,932 - MMP_RS05270 0.54 -0.8 1,007,933 + MMP_RS05270 0.55 +1.1 1,007,934 - MMP_RS05270 0.55 +0.4 1,007,961 - MMP_RS05270 0.58 -0.8 1,007,961 - MMP_RS05270 0.58 -1.1 1,007,961 - MMP_RS05270 0.58 -2.4 1,007,962 + MMP_RS05270 0.58 +0.2 1,007,962 + MMP_RS05270 0.58 -2.1 1,007,963 - MMP_RS05270 0.58 -0.8 1,007,963 - MMP_RS05270 0.58 -0.4 1,007,963 - MMP_RS05270 0.58 -1.1 1,007,964 + MMP_RS05270 0.58 -0.8 1,007,979 + MMP_RS05270 0.60 -0.6 1,008,079 + MMP_RS05270 0.73 -0.7 1,008,096 + MMP_RS05270 0.75 -0.8 1,008,148 - MMP_RS05270 0.81 +0.2 1,008,150 - MMP_RS05270 0.82 -0.2 1,008,155 + MMP_RS05270 0.82 -0.7 1,008,167 + MMP_RS05270 0.84 +0.6 1,008,217 - -1.5 1,008,259 + -1.3 1,008,302 - -3.7 1,008,347 - +1.0 1,008,560 - MMP_RS05275 0.27 +1.6 1,008,561 + MMP_RS05275 0.27 -0.8 1,008,565 - MMP_RS05275 0.27 -0.4 1,008,565 - MMP_RS05275 0.27 +0.4 1,008,565 - MMP_RS05275 0.27 -1.3 1,008,581 - MMP_RS05275 0.29 -0.7 1,008,582 + MMP_RS05275 0.29 -0.4 1,008,582 + MMP_RS05275 0.29 -0.9 1,008,582 + MMP_RS05275 0.29 -2.2 1,008,620 + MMP_RS05275 0.33 -0.7 1,008,642 - MMP_RS05275 0.35 +0.4 1,008,771 - MMP_RS05275 0.49 +0.1 1,008,797 - MMP_RS05275 0.51 +0.6 1,008,810 - MMP_RS05275 0.53 -1.8 1,008,979 - MMP_RS05275 0.70 +1.3 1,009,029 - MMP_RS05275 0.75 -1.0 1,009,052 + MMP_RS05275 0.78 +0.0 1,009,060 - MMP_RS05275 0.79 -0.9 1,009,093 - MMP_RS05275 0.82 +1.3 1,009,213 + +1.0
Or see this region's nucleotide sequence