Strain Fitness in Methanococcus maripaludis S2 around MMP_RS03445

Experiment: McCas_formate_complete nitrate 35.7142857142857 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS03435 and MMP_RS03440 are separated by 57 nucleotidesMMP_RS03440 and MMP_RS03445 are separated by 75 nucleotidesMMP_RS03445 and MMP_RS03450 are separated by 74 nucleotidesMMP_RS03450 and MMP_RS03455 are separated by 97 nucleotides MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721 _RS03435 MMP_RS03440: MMP_RS03440 - MoaD/ThiS family protein, at 646,779 to 646,973 _RS03440 MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600 _RS03445 MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS (from data), at 647,675 to 647,947 _RS03450 MMP_RS03455: MMP_RS03455 - radical SAM protein, at 648,045 to 648,938 _RS03455 Position (kb) 647 648Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 646.615 kb on - strand, within MMP_RS03435at 646.660 kb on - strandat 646.720 kb on + strandat 646.870 kb on - strand, within MMP_RS03440at 646.979 kb on - strandat 646.983 kb on - strandat 646.984 kb on + strandat 646.987 kb on - strandat 646.997 kb on + strandat 647.001 kb on - strandat 647.002 kb on + strandat 647.002 kb on + strandat 647.004 kb on + strandat 647.018 kb on - strandat 647.055 kb on - strandat 647.407 kb on - strand, within MMP_RS03445at 647.695 kb on - strandat 647.797 kb on - strand, within MMP_RS03450at 647.797 kb on - strand, within MMP_RS03450at 647.852 kb on - strand, within MMP_RS03450at 647.951 kb on - strandat 647.951 kb on - strandat 648.008 kb on - strandat 648.008 kb on - strandat 648.009 kb on + strandat 648.015 kb on - strandat 648.086 kb on - strandat 648.087 kb on + strandat 648.117 kb on - strandat 648.186 kb on + strandat 648.261 kb on - strandat 648.262 kb on + strandat 648.262 kb on + strandat 648.508 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete nitrate 35.7142857142857 mM
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646,615 - MMP_RS03435 0.89 +0.5
646,660 - -0.1
646,720 + -0.6
646,870 - MMP_RS03440 0.47 +0.5
646,979 - -0.8
646,983 - +0.6
646,984 + +0.2
646,987 - +0.1
646,997 + +0.1
647,001 - +0.4
647,002 + -0.9
647,002 + -0.7
647,004 + -0.6
647,018 - +1.1
647,055 - +0.8
647,407 - MMP_RS03445 0.65 -1.7
647,695 - -2.0
647,797 - MMP_RS03450 0.45 +0.1
647,797 - MMP_RS03450 0.45 -0.9
647,852 - MMP_RS03450 0.65 -2.6
647,951 - -2.4
647,951 - -2.8
648,008 - -3.7
648,008 - -1.9
648,009 + -0.0
648,015 - -1.0
648,086 - +2.4
648,087 + +0.2
648,117 - +0.2
648,186 + -1.2
648,261 - -0.2
648,262 + +0.0
648,262 + -1.1
648,508 + -0.4

Or see this region's nucleotide sequence