Experiment: McCas_formate_complete NO 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06670 and MMP_RS06675 are separated by 24 nucleotides MMP_RS06675 and MMP_RS06680 are separated by 23 nucleotides
MMP_RS06670: MMP_RS06670 - glucose-6-phosphate isomerase, at 1,279,866 to 1,281,182
_RS06670
MMP_RS06675: MMP_RS06675 - ADP-specific phosphofructokinase, at 1,281,207 to 1,282,598
_RS06675
MMP_RS06680: MMP_RS06680 - Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal domain, at 1,282,622 to 1,283,785
_RS06680
Position (kb)
1281
1282
1283 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1280.541 kb on + strand, within MMP_RS06670 at 1280.612 kb on - strand, within MMP_RS06670 at 1280.612 kb on - strand, within MMP_RS06670 at 1280.784 kb on - strand, within MMP_RS06670 at 1280.828 kb on + strand, within MMP_RS06670 at 1280.837 kb on - strand, within MMP_RS06670 at 1280.837 kb on - strand, within MMP_RS06670 at 1280.838 kb on + strand, within MMP_RS06670 at 1280.838 kb on + strand, within MMP_RS06670 at 1281.148 kb on + strand at 1281.150 kb on + strand at 1281.151 kb on - strand at 1281.152 kb on + strand at 1281.162 kb on - strand at 1281.169 kb on - strand at 1281.170 kb on + strand at 1281.170 kb on + strand at 1281.218 kb on - strand at 1281.219 kb on + strand at 1281.257 kb on + strand at 1281.261 kb on - strand at 1281.261 kb on - strand at 1281.261 kb on - strand at 1281.262 kb on + strand at 1281.319 kb on - strand at 1281.496 kb on + strand, within MMP_RS06675 at 1281.496 kb on + strand, within MMP_RS06675 at 1281.949 kb on - strand, within MMP_RS06675 at 1282.241 kb on - strand, within MMP_RS06675 at 1282.242 kb on + strand, within MMP_RS06675 at 1282.256 kb on + strand, within MMP_RS06675 at 1282.256 kb on + strand, within MMP_RS06675 at 1282.261 kb on + strand, within MMP_RS06675 at 1282.261 kb on + strand, within MMP_RS06675 at 1282.261 kb on + strand, within MMP_RS06675 at 1282.337 kb on - strand, within MMP_RS06675 at 1282.396 kb on - strand, within MMP_RS06675 at 1282.403 kb on - strand, within MMP_RS06675 at 1282.407 kb on + strand, within MMP_RS06675 at 1282.424 kb on + strand, within MMP_RS06675 at 1282.535 kb on - strand at 1282.558 kb on - strand at 1282.563 kb on - strand at 1282.600 kb on - strand at 1282.747 kb on - strand, within MMP_RS06680 at 1282.754 kb on - strand, within MMP_RS06680 at 1282.754 kb on - strand, within MMP_RS06680 at 1282.867 kb on - strand, within MMP_RS06680 at 1282.901 kb on - strand, within MMP_RS06680 at 1282.901 kb on - strand, within MMP_RS06680 at 1282.901 kb on - strand, within MMP_RS06680 at 1282.901 kb on - strand, within MMP_RS06680 at 1282.902 kb on + strand, within MMP_RS06680 at 1282.928 kb on + strand, within MMP_RS06680 at 1283.339 kb on - strand, within MMP_RS06680 at 1283.364 kb on - strand, within MMP_RS06680 at 1283.432 kb on + strand, within MMP_RS06680 at 1283.432 kb on + strand, within MMP_RS06680 at 1283.432 kb on + strand, within MMP_RS06680 at 1283.433 kb on - strand, within MMP_RS06680 at 1283.435 kb on - strand, within MMP_RS06680 at 1283.435 kb on - strand, within MMP_RS06680 at 1283.435 kb on - strand, within MMP_RS06680 at 1283.435 kb on - strand, within MMP_RS06680 at 1283.436 kb on + strand, within MMP_RS06680 at 1283.436 kb on + strand, within MMP_RS06680 at 1283.528 kb on - strand, within MMP_RS06680
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete NO 0.025 mM remove 1,280,541 + MMP_RS06670 0.51 -3.4 1,280,612 - MMP_RS06670 0.57 -1.1 1,280,612 - MMP_RS06670 0.57 -1.5 1,280,784 - MMP_RS06670 0.70 -1.3 1,280,828 + MMP_RS06670 0.73 -3.1 1,280,837 - MMP_RS06670 0.74 +0.5 1,280,837 - MMP_RS06670 0.74 -2.4 1,280,838 + MMP_RS06670 0.74 -2.2 1,280,838 + MMP_RS06670 0.74 +0.0 1,281,148 + -1.2 1,281,150 + +0.2 1,281,151 - -0.7 1,281,152 + -1.6 1,281,162 - -0.7 1,281,169 - -0.8 1,281,170 + -0.6 1,281,170 + +1.0 1,281,218 - -0.8 1,281,219 + -1.3 1,281,257 + +1.5 1,281,261 - -1.2 1,281,261 - -1.2 1,281,261 - -1.4 1,281,262 + -2.1 1,281,319 - -1.3 1,281,496 + MMP_RS06675 0.21 -1.9 1,281,496 + MMP_RS06675 0.21 -0.8 1,281,949 - MMP_RS06675 0.53 +0.2 1,282,241 - MMP_RS06675 0.74 -0.5 1,282,242 + MMP_RS06675 0.74 -1.8 1,282,256 + MMP_RS06675 0.75 +0.6 1,282,256 + MMP_RS06675 0.75 -2.3 1,282,261 + MMP_RS06675 0.76 -1.0 1,282,261 + MMP_RS06675 0.76 -1.9 1,282,261 + MMP_RS06675 0.76 -0.6 1,282,337 - MMP_RS06675 0.81 -1.2 1,282,396 - MMP_RS06675 0.85 -1.5 1,282,403 - MMP_RS06675 0.86 -0.9 1,282,407 + MMP_RS06675 0.86 -1.7 1,282,424 + MMP_RS06675 0.87 -1.7 1,282,535 - -2.0 1,282,558 - -1.8 1,282,563 - -0.2 1,282,600 - +1.1 1,282,747 - MMP_RS06680 0.11 -2.0 1,282,754 - MMP_RS06680 0.11 -4.3 1,282,754 - MMP_RS06680 0.11 -0.9 1,282,867 - MMP_RS06680 0.21 -1.3 1,282,901 - MMP_RS06680 0.24 -3.0 1,282,901 - MMP_RS06680 0.24 -3.7 1,282,901 - MMP_RS06680 0.24 -3.4 1,282,901 - MMP_RS06680 0.24 -2.3 1,282,902 + MMP_RS06680 0.24 -2.4 1,282,928 + MMP_RS06680 0.26 -3.9 1,283,339 - MMP_RS06680 0.62 -2.5 1,283,364 - MMP_RS06680 0.64 -3.5 1,283,432 + MMP_RS06680 0.70 -5.0 1,283,432 + MMP_RS06680 0.70 -2.4 1,283,432 + MMP_RS06680 0.70 -5.3 1,283,433 - MMP_RS06680 0.70 -2.7 1,283,435 - MMP_RS06680 0.70 -2.6 1,283,435 - MMP_RS06680 0.70 -3.8 1,283,435 - MMP_RS06680 0.70 -3.1 1,283,435 - MMP_RS06680 0.70 -2.9 1,283,436 + MMP_RS06680 0.70 -4.2 1,283,436 + MMP_RS06680 0.70 -3.9 1,283,528 - MMP_RS06680 0.78 -4.1
Or see this region's nucleotide sequence