Experiment: McCas_formate_complete sulfate 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02845 and MMP_RS02850 are separated by 164 nucleotides MMP_RS02850 and MMP_RS02855 are separated by 180 nucleotides MMP_RS02855 and MMP_RS02860 are separated by 98 nucleotides MMP_RS02860 and MMP_RS02865 are separated by 36 nucleotides
MMP_RS02845: MMP_RS02845 - hypothetical protein, at 542,666 to 543,277
_RS02845
MMP_RS02850: MMP_RS02850 - hypothetical protein, at 543,442 to 543,732
_RS02850
MMP_RS02855: MMP_RS02855 - 3-isopropylmalate dehydrogenase, at 543,913 to 544,905
_RS02855
MMP_RS02860: MMP_RS02860 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 545,004 to 545,744
_RS02860
MMP_RS02865: MMP_RS02865 - phosphoserine phosphatase SerB, at 545,781 to 546,422
_RS02865
Position (kb) 543
544
545 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 543.047 kb on + strand, within MMP_RS02845 at 543.057 kb on - strand, within MMP_RS02845 at 543.068 kb on - strand, within MMP_RS02845 at 543.119 kb on + strand, within MMP_RS02845 at 543.175 kb on + strand, within MMP_RS02845 at 543.179 kb on - strand, within MMP_RS02845 at 543.193 kb on - strand, within MMP_RS02845 at 543.193 kb on - strand, within MMP_RS02845 at 543.196 kb on + strand, within MMP_RS02845 at 543.196 kb on + strand, within MMP_RS02845 at 543.238 kb on - strand at 543.241 kb on - strand at 543.242 kb on + strand at 543.242 kb on + strand at 543.243 kb on - strand at 543.276 kb on + strand at 543.300 kb on - strand at 543.321 kb on + strand at 543.331 kb on + strand at 543.337 kb on + strand at 543.361 kb on + strand at 543.364 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.370 kb on + strand at 543.370 kb on + strand at 543.370 kb on + strand at 543.370 kb on + strand at 543.370 kb on + strand at 543.371 kb on - strand at 543.371 kb on - strand at 543.372 kb on + strand at 543.373 kb on - strand at 543.373 kb on - strand at 543.373 kb on - strand at 543.373 kb on - strand at 543.373 kb on - strand at 543.373 kb on - strand at 543.374 kb on + strand at 543.374 kb on + strand at 543.374 kb on + strand at 543.374 kb on + strand at 543.401 kb on - strand at 543.403 kb on - strand at 543.404 kb on + strand at 543.439 kb on + strand at 543.492 kb on - strand, within MMP_RS02850 at 543.492 kb on - strand, within MMP_RS02850 at 543.492 kb on - strand, within MMP_RS02850 at 543.493 kb on + strand, within MMP_RS02850 at 543.493 kb on + strand, within MMP_RS02850 at 543.494 kb on - strand, within MMP_RS02850 at 543.495 kb on + strand, within MMP_RS02850 at 543.547 kb on + strand, within MMP_RS02850 at 543.547 kb on + strand, within MMP_RS02850 at 543.548 kb on - strand, within MMP_RS02850 at 543.548 kb on - strand, within MMP_RS02850 at 543.548 kb on - strand, within MMP_RS02850 at 543.549 kb on + strand, within MMP_RS02850 at 543.549 kb on + strand, within MMP_RS02850 at 543.549 kb on + strand, within MMP_RS02850 at 543.550 kb on - strand, within MMP_RS02850 at 543.551 kb on + strand, within MMP_RS02850 at 543.551 kb on + strand, within MMP_RS02850 at 543.551 kb on + strand, within MMP_RS02850 at 543.551 kb on + strand, within MMP_RS02850 at 543.551 kb on + strand, within MMP_RS02850 at 543.659 kb on + strand, within MMP_RS02850 at 543.859 kb on - strand at 544.055 kb on - strand at 544.181 kb on - strand at 544.198 kb on - strand at 544.235 kb on + strand at 544.239 kb on + strand at 544.241 kb on - strand at 544.494 kb on - strand at 544.513 kb on - strand at 544.772 kb on - strand at 544.923 kb on + strand at 544.945 kb on + strand at 544.951 kb on - strand at 544.952 kb on + strand at 544.952 kb on + strand at 544.964 kb on - strand at 544.965 kb on + strand at 545.248 kb on + strand at 545.430 kb on + strand at 545.612 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sulfate 125 mM remove 543,047 + MMP_RS02845 0.62 +1.1 543,057 - MMP_RS02845 0.64 +0.4 543,068 - MMP_RS02845 0.66 -1.3 543,119 + MMP_RS02845 0.74 -0.4 543,175 + MMP_RS02845 0.83 -2.0 543,179 - MMP_RS02845 0.84 +0.8 543,193 - MMP_RS02845 0.86 -0.5 543,193 - MMP_RS02845 0.86 +0.5 543,196 + MMP_RS02845 0.87 +0.2 543,196 + MMP_RS02845 0.87 +0.9 543,238 - +1.3 543,241 - +0.8 543,242 + -3.5 543,242 + +0.8 543,243 - +0.3 543,276 + +0.2 543,300 - +2.0 543,321 + +0.4 543,331 + +0.6 543,337 + +1.7 543,361 + +0.5 543,364 - -0.7 543,367 - -1.2 543,367 - +1.5 543,369 - -0.8 543,369 - +1.9 543,369 - +1.4 543,369 - -2.5 543,369 - +0.5 543,369 - +0.4 543,369 - +0.8 543,370 + -4.1 543,370 + +1.5 543,370 + -0.7 543,370 + -0.2 543,370 + +1.2 543,371 - -3.2 543,371 - +1.9 543,372 + +0.7 543,373 - +0.2 543,373 - -1.5 543,373 - -3.3 543,373 - -1.0 543,373 - +0.1 543,373 - +0.1 543,374 + +0.4 543,374 + +0.3 543,374 + +2.2 543,374 + +0.7 543,401 - +1.6 543,403 - -0.8 543,404 + -0.7 543,439 + -0.6 543,492 - MMP_RS02850 0.17 -2.6 543,492 - MMP_RS02850 0.17 +1.1 543,492 - MMP_RS02850 0.17 -1.2 543,493 + MMP_RS02850 0.18 +0.8 543,493 + MMP_RS02850 0.18 -2.9 543,494 - MMP_RS02850 0.18 +0.6 543,495 + MMP_RS02850 0.18 +1.7 543,547 + MMP_RS02850 0.36 +0.7 543,547 + MMP_RS02850 0.36 +1.7 543,548 - MMP_RS02850 0.36 +0.2 543,548 - MMP_RS02850 0.36 -0.1 543,548 - MMP_RS02850 0.36 -0.5 543,549 + MMP_RS02850 0.37 -1.2 543,549 + MMP_RS02850 0.37 +0.4 543,549 + MMP_RS02850 0.37 +1.5 543,550 - MMP_RS02850 0.37 +0.2 543,551 + MMP_RS02850 0.37 +2.1 543,551 + MMP_RS02850 0.37 -0.9 543,551 + MMP_RS02850 0.37 +1.1 543,551 + MMP_RS02850 0.37 -0.8 543,551 + MMP_RS02850 0.37 +0.7 543,659 + MMP_RS02850 0.75 +0.7 543,859 - +0.3 544,055 - +0.8 544,181 - -0.1 544,198 - -0.7 544,235 + +1.8 544,239 + -0.8 544,241 - -0.4 544,494 - +2.9 544,513 - -0.9 544,772 - -2.7 544,923 + +1.9 544,945 + -3.7 544,951 - +2.6 544,952 + +1.2 544,952 + +2.6 544,964 - -0.5 544,965 + +2.4 545,248 + -1.5 545,430 + -1.0 545,612 + -1.5
Or see this region's nucleotide sequence