Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08765 and MMP_RS08985 are separated by 96 nucleotides MMP_RS08985 and MMP_RS08770 are separated by 22 nucleotides MMP_RS08770 and MMP_RS08775 overlap by 4 nucleotides MMP_RS08775 and MMP_RS08780 are separated by 9 nucleotides
MMP_RS08765: MMP_RS08765 - homoserine dehydrogenase, at 1,642,986 to 1,643,999
_RS08765
MMP_RS08985: MMP_RS08985 - tRNA-Sec, at 1,644,096 to 1,644,185
_RS08985
MMP_RS08770: MMP_RS08770 - hypothetical protein, at 1,644,208 to 1,645,104
_RS08770
MMP_RS08775: MMP_RS08775 - methanogenesis marker 9 domain-containing protein, at 1,645,101 to 1,645,556
_RS08775
MMP_RS08780: MMP_RS08780 - 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase, at 1,645,566 to 1,646,231
_RS08780
Position (kb)
1644
1645
1646 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1643.315 kb on + strand at 1643.379 kb on + strand at 1643.425 kb on + strand at 1644.059 kb on - strand at 1644.059 kb on - strand at 1644.060 kb on + strand at 1644.062 kb on + strand at 1644.063 kb on - strand at 1644.261 kb on + strand at 1644.288 kb on - strand at 1644.408 kb on + strand, within MMP_RS08770 at 1644.417 kb on + strand, within MMP_RS08770 at 1644.441 kb on - strand, within MMP_RS08770 at 1644.563 kb on - strand, within MMP_RS08770 at 1644.571 kb on + strand, within MMP_RS08770 at 1644.683 kb on - strand, within MMP_RS08770 at 1644.684 kb on + strand, within MMP_RS08770 at 1644.710 kb on - strand, within MMP_RS08770 at 1644.769 kb on + strand, within MMP_RS08770 at 1644.861 kb on + strand, within MMP_RS08770 at 1644.867 kb on - strand, within MMP_RS08770 at 1644.870 kb on + strand, within MMP_RS08770 at 1645.014 kb on - strand, within MMP_RS08770 at 1645.088 kb on - strand at 1645.274 kb on + strand, within MMP_RS08775 at 1645.274 kb on + strand, within MMP_RS08775 at 1645.311 kb on + strand, within MMP_RS08775 at 1645.416 kb on - strand, within MMP_RS08775 at 1645.513 kb on + strand at 1645.556 kb on - strand at 1645.556 kb on - strand at 1645.559 kb on + strand at 1645.567 kb on - strand at 1645.740 kb on - strand at 1645.740 kb on - strand at 1645.804 kb on + strand at 1645.804 kb on + strand at 1645.838 kb on - strand at 1645.850 kb on - strand at 1645.851 kb on + strand at 1645.917 kb on - strand at 1645.919 kb on - strand at 1645.967 kb on + strand at 1645.980 kb on + strand at 1646.021 kb on + strand at 1646.072 kb on - strand at 1646.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,643,315 + -2.4 1,643,379 + -4.1 1,643,425 + -3.9 1,644,059 - +2.3 1,644,059 - +1.1 1,644,060 + +2.5 1,644,062 + +1.0 1,644,063 - -2.6 1,644,261 + -0.2 1,644,288 - +0.6 1,644,408 + MMP_RS08770 0.22 +0.9 1,644,417 + MMP_RS08770 0.23 -0.7 1,644,441 - MMP_RS08770 0.26 +1.1 1,644,563 - MMP_RS08770 0.40 -1.9 1,644,571 + MMP_RS08770 0.40 +0.4 1,644,683 - MMP_RS08770 0.53 +4.5 1,644,684 + MMP_RS08770 0.53 -3.7 1,644,710 - MMP_RS08770 0.56 +1.1 1,644,769 + MMP_RS08770 0.63 +1.4 1,644,861 + MMP_RS08770 0.73 +1.0 1,644,867 - MMP_RS08770 0.73 +0.1 1,644,870 + MMP_RS08770 0.74 +1.3 1,645,014 - MMP_RS08770 0.90 -0.2 1,645,088 - +1.0 1,645,274 + MMP_RS08775 0.38 +1.1 1,645,274 + MMP_RS08775 0.38 +2.1 1,645,311 + MMP_RS08775 0.46 +1.4 1,645,416 - MMP_RS08775 0.69 +0.4 1,645,513 + +1.5 1,645,556 - -1.4 1,645,556 - +0.8 1,645,559 + +1.0 1,645,567 - +1.6 1,645,740 - -2.8 1,645,740 - -4.7 1,645,804 + -1.0 1,645,804 + -1.4 1,645,838 - -3.7 1,645,850 - -2.8 1,645,851 + -4.0 1,645,917 - -1.8 1,645,919 - -2.3 1,645,967 + -3.0 1,645,980 + -4.8 1,646,021 + -2.9 1,646,072 - -4.0 1,646,095 - -1.2
Or see this region's nucleotide sequence