Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06825 and MMP_RS06830 are separated by 20 nucleotides MMP_RS06830 and MMP_RS06835 are separated by 13 nucleotides MMP_RS06835 and MMP_RS06840 are separated by 38 nucleotides MMP_RS06840 and MMP_RS06845 are separated by 37 nucleotides MMP_RS06845 and MMP_RS06850 are separated by 37 nucleotides MMP_RS06850 and MMP_RS06855 are separated by 31 nucleotides
MMP_RS06825: MMP_RS06825 - 50S ribosomal protein L18e, at 1,307,955 to 1,308,317
_RS06825
MMP_RS06830: MMP_RS06830 - 50S ribosomal protein L13, at 1,308,338 to 1,308,751
_RS06830
MMP_RS06835: MMP_RS06835 - 30S ribosomal protein S9, at 1,308,765 to 1,309,169
_RS06835
MMP_RS06840: MMP_RS06840 - DNA-directed RNA polymerase subunit N, at 1,309,208 to 1,309,414
_RS06840
MMP_RS06845: MMP_RS06845 - tRNA-Pro, at 1,309,452 to 1,309,525
_RS06845
MMP_RS06850: MMP_RS06850 - DNA-directed RNA polymerase subunit K, at 1,309,563 to 1,309,736
_RS06850
MMP_RS06855: MMP_RS06855 - hypothetical protein, at 1,309,768 to 1,310,703
_RS06855
Position (kb)
1309
1310 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1309.168 kb on + strand at 1309.387 kb on + strand, within MMP_RS06840 at 1309.407 kb on + strand at 1309.425 kb on + strand at 1309.432 kb on - strand at 1309.436 kb on + strand at 1309.540 kb on + strand at 1309.672 kb on - strand at 1309.742 kb on - strand at 1309.836 kb on + strand at 1309.846 kb on + strand at 1309.870 kb on - strand, within MMP_RS06855 at 1309.925 kb on - strand, within MMP_RS06855 at 1309.957 kb on - strand, within MMP_RS06855 at 1309.985 kb on - strand, within MMP_RS06855 at 1310.195 kb on - strand, within MMP_RS06855 at 1310.199 kb on - strand, within MMP_RS06855 at 1310.199 kb on - strand, within MMP_RS06855 at 1310.204 kb on + strand, within MMP_RS06855 at 1310.266 kb on + strand, within MMP_RS06855 at 1310.319 kb on - strand, within MMP_RS06855 at 1310.319 kb on - strand, within MMP_RS06855 at 1310.374 kb on + strand, within MMP_RS06855 at 1310.402 kb on - strand, within MMP_RS06855
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,309,168 + -3.2 1,309,387 + MMP_RS06840 0.86 -1.6 1,309,407 + -3.3 1,309,425 + -3.2 1,309,432 - -1.6 1,309,436 + -0.7 1,309,540 + -2.9 1,309,672 - -1.9 1,309,742 - -1.8 1,309,836 + -4.5 1,309,846 + -1.9 1,309,870 - MMP_RS06855 0.11 -1.1 1,309,925 - MMP_RS06855 0.17 -2.4 1,309,957 - MMP_RS06855 0.20 -0.7 1,309,985 - MMP_RS06855 0.23 -1.7 1,310,195 - MMP_RS06855 0.46 -4.7 1,310,199 - MMP_RS06855 0.46 -1.7 1,310,199 - MMP_RS06855 0.46 -3.0 1,310,204 + MMP_RS06855 0.47 -5.1 1,310,266 + MMP_RS06855 0.53 -2.8 1,310,319 - MMP_RS06855 0.59 -2.0 1,310,319 - MMP_RS06855 0.59 -2.3 1,310,374 + MMP_RS06855 0.65 -2.5 1,310,402 - MMP_RS06855 0.68 -2.6
Or see this region's nucleotide sequence