Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06030 and MMP_RS06035 are separated by 118 nucleotides MMP_RS06035 and MMP_RS06040 are separated by 11 nucleotides MMP_RS06040 and MMP_RS06045 are separated by 29 nucleotides
MMP_RS06030: MMP_RS06030 - SufD family Fe-S cluster assembly protein, at 1,152,534 to 1,153,790
_RS06030
MMP_RS06035: MMP_RS06035 - glycosyltransferase family 2 protein, at 1,153,909 to 1,154,601
_RS06035
MMP_RS06040: MMP_RS06040 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,154,613 to 1,155,227
_RS06040
MMP_RS06045: MMP_RS06045 - DNA polymerase, at 1,155,257 to 1,155,808
_RS06045
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1153.048 kb on - strand at 1153.049 kb on + strand at 1153.062 kb on + strand at 1153.064 kb on - strand at 1153.143 kb on + strand at 1153.220 kb on - strand at 1153.221 kb on + strand at 1153.319 kb on + strand at 1153.454 kb on + strand at 1153.661 kb on - strand at 1153.688 kb on + strand at 1153.756 kb on - strand at 1153.785 kb on + strand at 1153.788 kb on - strand at 1153.788 kb on - strand at 1153.828 kb on - strand at 1153.843 kb on - strand at 1153.847 kb on - strand at 1153.848 kb on + strand at 1153.864 kb on - strand at 1153.864 kb on - strand at 1153.865 kb on + strand at 1153.869 kb on + strand at 1153.906 kb on + strand at 1153.935 kb on + strand at 1153.946 kb on + strand at 1154.016 kb on + strand, within MMP_RS06035 at 1154.016 kb on + strand, within MMP_RS06035 at 1154.070 kb on + strand, within MMP_RS06035 at 1154.089 kb on + strand, within MMP_RS06035 at 1154.091 kb on - strand, within MMP_RS06035 at 1154.144 kb on + strand, within MMP_RS06035 at 1154.157 kb on + strand, within MMP_RS06035 at 1154.222 kb on - strand, within MMP_RS06035 at 1154.257 kb on + strand, within MMP_RS06035 at 1154.287 kb on + strand, within MMP_RS06035 at 1154.335 kb on - strand, within MMP_RS06035 at 1154.340 kb on + strand, within MMP_RS06035 at 1154.433 kb on - strand, within MMP_RS06035 at 1154.437 kb on - strand, within MMP_RS06035 at 1154.444 kb on - strand, within MMP_RS06035 at 1154.445 kb on + strand, within MMP_RS06035 at 1154.445 kb on + strand, within MMP_RS06035 at 1154.515 kb on - strand, within MMP_RS06035 at 1154.516 kb on + strand, within MMP_RS06035 at 1154.516 kb on + strand, within MMP_RS06035 at 1154.518 kb on + strand, within MMP_RS06035 at 1154.608 kb on - strand at 1154.653 kb on - strand at 1154.663 kb on + strand at 1154.669 kb on - strand at 1154.838 kb on - strand at 1154.877 kb on + strand at 1155.033 kb on - strand at 1155.034 kb on + strand at 1155.077 kb on + strand at 1155.077 kb on + strand at 1155.086 kb on - strand at 1155.086 kb on - strand at 1155.197 kb on + strand at 1155.197 kb on + strand at 1155.209 kb on + strand at 1155.209 kb on + strand at 1155.209 kb on + strand at 1155.243 kb on + strand at 1155.249 kb on - strand at 1155.250 kb on + strand at 1155.250 kb on + strand at 1155.250 kb on + strand at 1155.253 kb on - strand at 1155.372 kb on + strand, within MMP_RS06045 at 1155.375 kb on + strand, within MMP_RS06045 at 1155.420 kb on + strand, within MMP_RS06045 at 1155.483 kb on + strand, within MMP_RS06045 at 1155.488 kb on + strand, within MMP_RS06045 at 1155.538 kb on - strand, within MMP_RS06045
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,153,048 - -0.7 1,153,049 + +0.3 1,153,062 + -0.7 1,153,064 - -2.9 1,153,143 + -1.3 1,153,220 - -3.0 1,153,221 + -1.7 1,153,319 + -1.7 1,153,454 + -2.9 1,153,661 - -2.0 1,153,688 + -3.0 1,153,756 - -0.3 1,153,785 + +1.2 1,153,788 - +0.6 1,153,788 - +0.3 1,153,828 - +0.1 1,153,843 - +0.9 1,153,847 - -2.9 1,153,848 + -0.4 1,153,864 - -3.0 1,153,864 - -2.2 1,153,865 + -1.2 1,153,869 + -0.0 1,153,906 + -1.7 1,153,935 + -5.0 1,153,946 + -3.1 1,154,016 + MMP_RS06035 0.15 -3.7 1,154,016 + MMP_RS06035 0.15 -3.0 1,154,070 + MMP_RS06035 0.23 -3.0 1,154,089 + MMP_RS06035 0.26 -3.4 1,154,091 - MMP_RS06035 0.26 -1.6 1,154,144 + MMP_RS06035 0.34 -3.1 1,154,157 + MMP_RS06035 0.36 -2.7 1,154,222 - MMP_RS06035 0.45 -4.3 1,154,257 + MMP_RS06035 0.50 -4.6 1,154,287 + MMP_RS06035 0.55 -2.9 1,154,335 - MMP_RS06035 0.61 -1.3 1,154,340 + MMP_RS06035 0.62 -4.2 1,154,433 - MMP_RS06035 0.76 -2.9 1,154,437 - MMP_RS06035 0.76 -1.8 1,154,444 - MMP_RS06035 0.77 -1.5 1,154,445 + MMP_RS06035 0.77 -2.6 1,154,445 + MMP_RS06035 0.77 -3.5 1,154,515 - MMP_RS06035 0.87 -3.1 1,154,516 + MMP_RS06035 0.88 -1.8 1,154,516 + MMP_RS06035 0.88 -4.3 1,154,518 + MMP_RS06035 0.88 -0.2 1,154,608 - -1.0 1,154,653 - -0.7 1,154,663 + -2.9 1,154,669 - +0.4 1,154,838 - +0.7 1,154,877 + +0.4 1,155,033 - +1.8 1,155,034 + -1.9 1,155,077 + +0.2 1,155,077 + -1.1 1,155,086 - -0.8 1,155,086 - -0.3 1,155,197 + -0.9 1,155,197 + +1.8 1,155,209 + +1.6 1,155,209 + +0.8 1,155,209 + +0.6 1,155,243 + -0.7 1,155,249 - +0.6 1,155,250 + +0.6 1,155,250 + +1.1 1,155,250 + -0.8 1,155,253 - +1.0 1,155,372 + MMP_RS06045 0.21 -0.7 1,155,375 + MMP_RS06045 0.21 +1.1 1,155,420 + MMP_RS06045 0.30 +0.4 1,155,483 + MMP_RS06045 0.41 +0.6 1,155,488 + MMP_RS06045 0.42 -1.1 1,155,538 - MMP_RS06045 0.51 +0.8
Or see this region's nucleotide sequence