Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05630 and MMP_RS05635 are separated by 30 nucleotides MMP_RS05635 and MMP_RS05640 are separated by 124 nucleotides
MMP_RS05630: MMP_RS05630 - flippase, at 1,081,000 to 1,082,502
_RS05630
MMP_RS05635: MMP_RS05635 - NAD-dependent epimerase/dehydratase family protein, at 1,082,533 to 1,083,411
_RS05635
MMP_RS05640: MMP_RS05640 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,083,536 to 1,084,384
_RS05640
Position (kb)
1082
1083
1084 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 1081.631 kb on + strand at 1081.663 kb on - strand at 1081.865 kb on + strand at 1081.895 kb on + strand at 1081.921 kb on + strand at 1081.958 kb on - strand at 1081.990 kb on - strand at 1082.085 kb on + strand at 1082.155 kb on - strand at 1082.160 kb on + strand at 1082.281 kb on - strand at 1082.331 kb on - strand at 1082.349 kb on - strand at 1082.427 kb on + strand at 1082.495 kb on - strand at 1082.534 kb on - strand at 1082.545 kb on + strand at 1082.590 kb on + strand at 1082.595 kb on - strand at 1082.785 kb on + strand, within MMP_RS05635 at 1082.859 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.956 kb on + strand, within MMP_RS05635 at 1082.972 kb on - strand, within MMP_RS05635 at 1082.972 kb on - strand, within MMP_RS05635 at 1083.024 kb on - strand, within MMP_RS05635 at 1083.088 kb on - strand, within MMP_RS05635 at 1083.108 kb on + strand, within MMP_RS05635 at 1083.217 kb on - strand, within MMP_RS05635 at 1083.309 kb on - strand, within MMP_RS05635 at 1083.314 kb on + strand, within MMP_RS05635 at 1083.352 kb on - strand at 1083.357 kb on - strand at 1083.358 kb on + strand at 1083.358 kb on + strand at 1083.397 kb on - strand at 1083.431 kb on - strand at 1083.431 kb on - strand at 1083.435 kb on + strand at 1083.435 kb on + strand at 1083.438 kb on - strand at 1083.438 kb on - strand at 1083.439 kb on + strand at 1083.439 kb on + strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.459 kb on + strand at 1083.459 kb on + strand at 1083.469 kb on - strand at 1083.469 kb on - strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.489 kb on + strand at 1083.489 kb on + strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.497 kb on - strand at 1083.497 kb on - strand at 1083.532 kb on - strand at 1083.561 kb on - strand at 1083.623 kb on + strand, within MMP_RS05640 at 1083.708 kb on + strand, within MMP_RS05640 at 1083.773 kb on - strand, within MMP_RS05640 at 1083.774 kb on + strand, within MMP_RS05640 at 1083.778 kb on + strand, within MMP_RS05640 at 1083.816 kb on + strand, within MMP_RS05640 at 1083.816 kb on + strand, within MMP_RS05640 at 1083.892 kb on - strand, within MMP_RS05640 at 1084.008 kb on + strand, within MMP_RS05640 at 1084.087 kb on - strand, within MMP_RS05640 at 1084.088 kb on + strand, within MMP_RS05640 at 1084.089 kb on - strand, within MMP_RS05640 at 1084.092 kb on - strand, within MMP_RS05640 at 1084.222 kb on - strand, within MMP_RS05640 at 1084.235 kb on + strand, within MMP_RS05640 at 1084.268 kb on - strand, within MMP_RS05640 at 1084.269 kb on + strand, within MMP_RS05640 at 1084.272 kb on - strand, within MMP_RS05640 at 1084.273 kb on + strand, within MMP_RS05640 at 1084.278 kb on + strand, within MMP_RS05640 at 1084.389 kb on - strand at 1084.405 kb on - strand at 1084.405 kb on - strand at 1084.411 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,081,631 + -3.1 1,081,663 - +1.1 1,081,865 + -3.2 1,081,895 + +4.7 1,081,921 + -0.1 1,081,958 - -1.1 1,081,990 - +2.1 1,082,085 + -3.4 1,082,155 - -2.9 1,082,160 + +1.6 1,082,281 - -3.1 1,082,331 - +2.5 1,082,349 - +0.8 1,082,427 + -2.9 1,082,495 - -0.1 1,082,534 - -1.7 1,082,545 + -1.5 1,082,590 + -2.9 1,082,595 - -3.0 1,082,785 + MMP_RS05635 0.29 -5.6 1,082,859 - MMP_RS05635 0.37 -3.5 1,082,948 - MMP_RS05635 0.47 -3.0 1,082,948 - MMP_RS05635 0.47 -1.9 1,082,948 - MMP_RS05635 0.47 -5.3 1,082,956 + MMP_RS05635 0.48 -2.1 1,082,972 - MMP_RS05635 0.50 -5.0 1,082,972 - MMP_RS05635 0.50 -2.8 1,083,024 - MMP_RS05635 0.56 -1.9 1,083,088 - MMP_RS05635 0.63 -2.0 1,083,108 + MMP_RS05635 0.65 -3.6 1,083,217 - MMP_RS05635 0.78 -5.5 1,083,309 - MMP_RS05635 0.88 -3.9 1,083,314 + MMP_RS05635 0.89 -3.4 1,083,352 - -3.1 1,083,357 - -1.2 1,083,358 + -3.1 1,083,358 + -3.7 1,083,397 - -1.3 1,083,431 - -1.0 1,083,431 - -3.0 1,083,435 + -3.9 1,083,435 + -1.9 1,083,438 - -3.1 1,083,438 - -1.6 1,083,439 + -1.4 1,083,439 + -2.6 1,083,458 - +1.6 1,083,458 - +0.3 1,083,458 - -2.4 1,083,459 + -1.9 1,083,459 + -3.6 1,083,469 - +1.7 1,083,469 - +0.2 1,083,472 + -1.5 1,083,472 + -5.5 1,083,472 + -0.0 1,083,489 + -0.5 1,083,489 + -1.8 1,083,490 - +5.2 1,083,490 - -0.7 1,083,490 - +0.6 1,083,491 + -0.6 1,083,491 + -1.7 1,083,491 + -0.7 1,083,491 + +0.9 1,083,497 - +1.3 1,083,497 - -0.5 1,083,532 - -1.2 1,083,561 - +2.0 1,083,623 + MMP_RS05640 0.10 -1.3 1,083,708 + MMP_RS05640 0.20 -1.3 1,083,773 - MMP_RS05640 0.28 +1.4 1,083,774 + MMP_RS05640 0.28 +5.4 1,083,778 + MMP_RS05640 0.29 +2.9 1,083,816 + MMP_RS05640 0.33 +0.5 1,083,816 + MMP_RS05640 0.33 +3.0 1,083,892 - MMP_RS05640 0.42 +3.1 1,084,008 + MMP_RS05640 0.56 +2.3 1,084,087 - MMP_RS05640 0.65 +3.5 1,084,088 + MMP_RS05640 0.65 +0.8 1,084,089 - MMP_RS05640 0.65 +5.1 1,084,092 - MMP_RS05640 0.65 +1.7 1,084,222 - MMP_RS05640 0.81 +0.5 1,084,235 + MMP_RS05640 0.82 -2.3 1,084,268 - MMP_RS05640 0.86 +2.9 1,084,269 + MMP_RS05640 0.86 +1.9 1,084,272 - MMP_RS05640 0.87 +4.4 1,084,273 + MMP_RS05640 0.87 -3.1 1,084,278 + MMP_RS05640 0.87 +1.8 1,084,389 - +0.3 1,084,405 - +2.3 1,084,405 - +1.0 1,084,411 + +1.6
Or see this region's nucleotide sequence