Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05280 and MMP_RS05285 overlap by 8 nucleotides MMP_RS05285 and MMP_RS05290 are separated by 83 nucleotides MMP_RS05290 and MMP_RS05295 are separated by 111 nucleotides
MMP_RS05280: MMP_RS05280 - COG1361 S-layer family protein, at 1,009,427 to 1,010,998
_RS05280
MMP_RS05285: MMP_RS05285 - MMPL family transporter, at 1,010,991 to 1,012,157
_RS05285
MMP_RS05290: MMP_RS05290 - TetR/AcrR family transcriptional regulator, at 1,012,241 to 1,012,843
_RS05290
MMP_RS05295: MMP_RS05295 - minichromosome maintenance protein MCM, at 1,012,955 to 1,015,087
_RS05295
Position (kb)
1010
1011
1012
1013 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1009.995 kb on + strand, within MMP_RS05280 at 1010.005 kb on + strand, within MMP_RS05280 at 1010.250 kb on + strand, within MMP_RS05280 at 1010.313 kb on + strand, within MMP_RS05280 at 1010.316 kb on - strand, within MMP_RS05280 at 1010.316 kb on - strand, within MMP_RS05280 at 1010.491 kb on - strand, within MMP_RS05280 at 1010.575 kb on - strand, within MMP_RS05280 at 1010.577 kb on - strand, within MMP_RS05280 at 1010.577 kb on - strand, within MMP_RS05280 at 1010.592 kb on - strand, within MMP_RS05280 at 1010.709 kb on - strand, within MMP_RS05280 at 1010.736 kb on + strand, within MMP_RS05280 at 1010.781 kb on + strand, within MMP_RS05280 at 1010.810 kb on - strand, within MMP_RS05280 at 1010.816 kb on - strand, within MMP_RS05280 at 1011.228 kb on - strand, within MMP_RS05285 at 1011.228 kb on - strand, within MMP_RS05285 at 1011.243 kb on - strand, within MMP_RS05285 at 1011.328 kb on + strand, within MMP_RS05285 at 1011.460 kb on - strand, within MMP_RS05285 at 1011.469 kb on + strand, within MMP_RS05285 at 1011.670 kb on - strand, within MMP_RS05285 at 1011.747 kb on - strand, within MMP_RS05285 at 1011.784 kb on - strand, within MMP_RS05285 at 1011.784 kb on - strand, within MMP_RS05285 at 1011.785 kb on + strand, within MMP_RS05285 at 1011.828 kb on + strand, within MMP_RS05285 at 1011.944 kb on - strand, within MMP_RS05285 at 1012.174 kb on + strand at 1012.197 kb on - strand at 1012.217 kb on - strand at 1012.226 kb on - strand at 1012.280 kb on + strand at 1012.280 kb on + strand at 1012.280 kb on + strand at 1012.280 kb on + strand at 1012.462 kb on - strand, within MMP_RS05290 at 1012.462 kb on - strand, within MMP_RS05290 at 1012.499 kb on - strand, within MMP_RS05290 at 1012.500 kb on + strand, within MMP_RS05290 at 1012.500 kb on + strand, within MMP_RS05290 at 1012.505 kb on + strand, within MMP_RS05290 at 1012.538 kb on + strand, within MMP_RS05290 at 1012.551 kb on + strand, within MMP_RS05290 at 1012.581 kb on + strand, within MMP_RS05290 at 1012.583 kb on - strand, within MMP_RS05290 at 1012.622 kb on - strand, within MMP_RS05290 at 1012.622 kb on - strand, within MMP_RS05290 at 1012.708 kb on - strand, within MMP_RS05290 at 1012.715 kb on + strand, within MMP_RS05290 at 1012.745 kb on + strand, within MMP_RS05290 at 1012.769 kb on - strand, within MMP_RS05290 at 1012.786 kb on + strand at 1012.837 kb on - strand at 1012.837 kb on - strand at 1012.838 kb on + strand at 1012.847 kb on + strand at 1012.851 kb on - strand at 1012.851 kb on - strand at 1012.851 kb on - strand at 1012.865 kb on - strand at 1012.866 kb on + strand at 1012.874 kb on - strand at 1012.874 kb on - strand at 1012.874 kb on - strand at 1012.888 kb on - strand at 1012.892 kb on - strand at 1012.900 kb on - strand at 1012.900 kb on - strand at 1012.940 kb on + strand at 1012.961 kb on - strand at 1012.962 kb on + strand at 1012.966 kb on - strand at 1012.966 kb on - strand at 1012.967 kb on + strand at 1012.971 kb on - strand at 1012.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,009,995 + MMP_RS05280 0.36 -0.8 1,010,005 + MMP_RS05280 0.37 -1.9 1,010,250 + MMP_RS05280 0.52 -1.6 1,010,313 + MMP_RS05280 0.56 -2.7 1,010,316 - MMP_RS05280 0.57 -3.1 1,010,316 - MMP_RS05280 0.57 -3.1 1,010,491 - MMP_RS05280 0.68 -5.1 1,010,575 - MMP_RS05280 0.73 -1.7 1,010,577 - MMP_RS05280 0.73 -0.8 1,010,577 - MMP_RS05280 0.73 -2.5 1,010,592 - MMP_RS05280 0.74 -3.6 1,010,709 - MMP_RS05280 0.82 -1.9 1,010,736 + MMP_RS05280 0.83 -2.2 1,010,781 + MMP_RS05280 0.86 -2.3 1,010,810 - MMP_RS05280 0.88 -3.8 1,010,816 - MMP_RS05280 0.88 -1.6 1,011,228 - MMP_RS05285 0.20 -2.8 1,011,228 - MMP_RS05285 0.20 -2.6 1,011,243 - MMP_RS05285 0.22 -3.3 1,011,328 + MMP_RS05285 0.29 -4.2 1,011,460 - MMP_RS05285 0.40 -4.7 1,011,469 + MMP_RS05285 0.41 -1.0 1,011,670 - MMP_RS05285 0.58 -1.3 1,011,747 - MMP_RS05285 0.65 -1.5 1,011,784 - MMP_RS05285 0.68 -3.6 1,011,784 - MMP_RS05285 0.68 -5.4 1,011,785 + MMP_RS05285 0.68 -2.5 1,011,828 + MMP_RS05285 0.72 -1.9 1,011,944 - MMP_RS05285 0.82 -2.6 1,012,174 + -1.4 1,012,197 - +0.5 1,012,217 - -0.3 1,012,226 - -2.1 1,012,280 + -0.7 1,012,280 + -2.8 1,012,280 + -0.9 1,012,280 + -2.9 1,012,462 - MMP_RS05290 0.37 -2.5 1,012,462 - MMP_RS05290 0.37 -1.0 1,012,499 - MMP_RS05290 0.43 +0.0 1,012,500 + MMP_RS05290 0.43 -1.2 1,012,500 + MMP_RS05290 0.43 -2.0 1,012,505 + MMP_RS05290 0.44 -3.3 1,012,538 + MMP_RS05290 0.49 -2.4 1,012,551 + MMP_RS05290 0.51 -1.3 1,012,581 + MMP_RS05290 0.56 -3.8 1,012,583 - MMP_RS05290 0.57 -0.9 1,012,622 - MMP_RS05290 0.63 -1.1 1,012,622 - MMP_RS05290 0.63 +0.1 1,012,708 - MMP_RS05290 0.77 -1.1 1,012,715 + MMP_RS05290 0.79 -0.7 1,012,745 + MMP_RS05290 0.84 -1.6 1,012,769 - MMP_RS05290 0.88 +0.4 1,012,786 + -0.2 1,012,837 - +0.2 1,012,837 - -1.0 1,012,838 + -4.9 1,012,847 + -1.4 1,012,851 - -2.3 1,012,851 - +0.4 1,012,851 - -3.1 1,012,865 - -1.6 1,012,866 + -1.2 1,012,874 - -1.9 1,012,874 - -0.4 1,012,874 - -5.1 1,012,888 - -1.9 1,012,892 - -0.6 1,012,900 - +1.7 1,012,900 - +1.2 1,012,940 + -2.8 1,012,961 - -2.5 1,012,962 + +0.7 1,012,966 - +2.1 1,012,966 - -2.3 1,012,967 + -1.6 1,012,971 - +0.4 1,012,992 + -3.5
Or see this region's nucleotide sequence