Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03435 and MMP_RS03440 are separated by 57 nucleotides MMP_RS03440 and MMP_RS03445 are separated by 75 nucleotides MMP_RS03445 and MMP_RS03450 are separated by 74 nucleotides MMP_RS03450 and MMP_RS03455 are separated by 97 nucleotides
MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721
_RS03435
MMP_RS03440: MMP_RS03440 - MoaD/ThiS family protein, at 646,779 to 646,973
_RS03440
MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600
_RS03445
MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS (from data) , at 647,675 to 647,947
_RS03450
MMP_RS03455: MMP_RS03455 - radical SAM protein, at 648,045 to 648,938
_RS03455
Position (kb)
647
648 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 646.615 kb on - strand, within MMP_RS03435 at 646.660 kb on - strand at 646.720 kb on + strand at 646.870 kb on - strand, within MMP_RS03440 at 646.979 kb on - strand at 646.983 kb on - strand at 646.984 kb on + strand at 646.987 kb on - strand at 646.997 kb on + strand at 647.001 kb on - strand at 647.002 kb on + strand at 647.002 kb on + strand at 647.004 kb on + strand at 647.018 kb on - strand at 647.055 kb on - strand at 647.407 kb on - strand, within MMP_RS03445 at 647.695 kb on - strand at 647.797 kb on - strand, within MMP_RS03450 at 647.797 kb on - strand, within MMP_RS03450 at 647.852 kb on - strand, within MMP_RS03450 at 647.951 kb on - strand at 647.951 kb on - strand at 648.008 kb on - strand at 648.008 kb on - strand at 648.009 kb on + strand at 648.015 kb on - strand at 648.086 kb on - strand at 648.087 kb on + strand at 648.117 kb on - strand at 648.186 kb on + strand at 648.261 kb on - strand at 648.262 kb on + strand at 648.262 kb on + strand at 648.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 646,615 - MMP_RS03435 0.89 -2.7 646,660 - -3.9 646,720 + -0.6 646,870 - MMP_RS03440 0.47 -0.6 646,979 - +0.2 646,983 - +0.6 646,984 + +1.4 646,987 - -4.1 646,997 + -0.6 647,001 - -1.6 647,002 + -1.7 647,002 + +0.7 647,004 + +0.0 647,018 - -1.1 647,055 - -0.5 647,407 - MMP_RS03445 0.65 -2.6 647,695 - -4.3 647,797 - MMP_RS03450 0.45 -0.4 647,797 - MMP_RS03450 0.45 -0.2 647,852 - MMP_RS03450 0.65 -0.6 647,951 - -3.3 647,951 - +0.4 648,008 - -1.7 648,008 - +1.0 648,009 + -0.2 648,015 - +1.4 648,086 - +1.5 648,087 + -3.6 648,117 - -0.2 648,186 + -3.5 648,261 - -2.5 648,262 + -2.2 648,262 + -3.3 648,508 + -2.7
Or see this region's nucleotide sequence