Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02015 and MMP_RS02020 are separated by 5 nucleotides MMP_RS02020 and MMP_RS02025 are separated by 4 nucleotides
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
MMP_RS02020: MMP_RS02020 - gamma-glutamylcyclotransferase, at 376,162 to 376,485
_RS02020
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
Position (kb)
376
377 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 375.479 kb on - strand, within MMP_RS02015 at 375.480 kb on + strand, within MMP_RS02015 at 375.518 kb on - strand, within MMP_RS02015 at 375.626 kb on + strand, within MMP_RS02015 at 375.665 kb on - strand, within MMP_RS02015 at 375.667 kb on - strand, within MMP_RS02015 at 375.835 kb on + strand, within MMP_RS02015 at 376.049 kb on + strand at 376.127 kb on + strand at 376.131 kb on - strand at 376.132 kb on + strand at 376.179 kb on + strand at 376.221 kb on - strand, within MMP_RS02020 at 376.300 kb on - strand, within MMP_RS02020 at 376.318 kb on + strand, within MMP_RS02020 at 376.385 kb on - strand, within MMP_RS02020 at 376.479 kb on + strand at 376.568 kb on - strand at 376.679 kb on - strand at 376.682 kb on + strand at 376.682 kb on + strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.730 kb on - strand, within MMP_RS02025 at 376.768 kb on - strand, within MMP_RS02025 at 376.789 kb on - strand, within MMP_RS02025 at 376.974 kb on + strand, within MMP_RS02025 at 377.239 kb on + strand, within MMP_RS02025
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 375,479 - MMP_RS02015 0.47 +0.4 375,480 + MMP_RS02015 0.47 -1.8 375,518 - MMP_RS02015 0.50 +0.4 375,626 + MMP_RS02015 0.59 +0.6 375,665 - MMP_RS02015 0.62 -0.5 375,667 - MMP_RS02015 0.62 -0.0 375,835 + MMP_RS02015 0.75 +0.3 376,049 + +1.1 376,127 + -1.3 376,131 - -0.7 376,132 + -0.0 376,179 + +0.5 376,221 - MMP_RS02020 0.18 -2.1 376,300 - MMP_RS02020 0.43 -0.5 376,318 + MMP_RS02020 0.48 -0.6 376,385 - MMP_RS02020 0.69 -1.8 376,479 + +0.5 376,568 - +0.4 376,679 - -2.6 376,682 + +0.7 376,682 + -3.8 376,691 - -0.6 376,691 - +3.2 376,691 - -1.7 376,730 - MMP_RS02025 0.10 -2.4 376,768 - MMP_RS02025 0.12 -0.4 376,789 - MMP_RS02025 0.13 -0.8 376,974 + MMP_RS02025 0.21 +0.5 377,239 + MMP_RS02025 0.32 -0.6
Or see this region's nucleotide sequence