Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01215 and MMP_RS01220 are separated by 78 nucleotides MMP_RS01220 and MMP_RS01225 are separated by 9 nucleotides MMP_RS01225 and MMP_RS01230 are separated by 9 nucleotides
MMP_RS01215: MMP_RS01215 - sodium/proline symporter PutP, at 229,031 to 230,509
_RS01215
MMP_RS01220: MMP_RS01220 - hypothetical protein, at 230,588 to 230,827
_RS01220
MMP_RS01225: MMP_RS01225 - DUF5379 domain-containing protein, at 230,837 to 231,115
_RS01225
MMP_RS01230: MMP_RS01230 - glutamate-1-semialdehyde 2 1-aminomutase, at 231,125 to 232,408
_RS01230
Position (kb) 230
231
232 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 229.965 kb on - strand, within MMP_RS01215 at 229.966 kb on + strand, within MMP_RS01215 at 230.437 kb on + strand at 230.544 kb on + strand at 230.544 kb on + strand at 230.544 kb on + strand at 230.547 kb on - strand at 230.548 kb on + strand at 230.557 kb on + strand at 230.557 kb on + strand at 230.588 kb on + strand at 230.637 kb on - strand, within MMP_RS01220 at 230.687 kb on + strand, within MMP_RS01220 at 230.705 kb on + strand, within MMP_RS01220 at 230.720 kb on - strand, within MMP_RS01220 at 230.724 kb on - strand, within MMP_RS01220 at 230.724 kb on - strand, within MMP_RS01220 at 230.743 kb on + strand, within MMP_RS01220 at 230.755 kb on - strand, within MMP_RS01220 at 230.756 kb on + strand, within MMP_RS01220 at 230.756 kb on + strand, within MMP_RS01220 at 230.756 kb on + strand, within MMP_RS01220 at 230.759 kb on - strand, within MMP_RS01220 at 230.760 kb on + strand, within MMP_RS01220 at 230.762 kb on + strand, within MMP_RS01220 at 230.762 kb on + strand, within MMP_RS01220 at 230.792 kb on + strand, within MMP_RS01220 at 230.844 kb on - strand at 230.844 kb on - strand at 230.906 kb on - strand, within MMP_RS01225 at 230.906 kb on - strand, within MMP_RS01225 at 230.912 kb on - strand, within MMP_RS01225 at 230.912 kb on - strand, within MMP_RS01225 at 230.912 kb on - strand, within MMP_RS01225 at 230.955 kb on - strand, within MMP_RS01225 at 230.974 kb on + strand, within MMP_RS01225 at 230.977 kb on - strand, within MMP_RS01225 at 230.977 kb on - strand, within MMP_RS01225 at 231.095 kb on - strand at 231.162 kb on - strand at 231.357 kb on - strand at 231.734 kb on + strand at 232.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 229,965 - MMP_RS01215 0.63 -0.9 229,966 + MMP_RS01215 0.63 +0.5 230,437 + +0.6 230,544 + +2.0 230,544 + +1.4 230,544 + +1.6 230,547 - +0.8 230,548 + +0.1 230,557 + +0.7 230,557 + +0.8 230,588 + -1.8 230,637 - MMP_RS01220 0.20 -0.1 230,687 + MMP_RS01220 0.41 -1.2 230,705 + MMP_RS01220 0.49 +1.2 230,720 - MMP_RS01220 0.55 -0.1 230,724 - MMP_RS01220 0.57 -1.8 230,724 - MMP_RS01220 0.57 -2.1 230,743 + MMP_RS01220 0.65 -3.0 230,755 - MMP_RS01220 0.70 +0.4 230,756 + MMP_RS01220 0.70 +1.2 230,756 + MMP_RS01220 0.70 -1.8 230,756 + MMP_RS01220 0.70 -0.5 230,759 - MMP_RS01220 0.71 -1.2 230,760 + MMP_RS01220 0.72 -0.3 230,762 + MMP_RS01220 0.72 -0.4 230,762 + MMP_RS01220 0.72 +2.8 230,792 + MMP_RS01220 0.85 -0.4 230,844 - -1.8 230,844 - -4.7 230,906 - MMP_RS01225 0.25 -3.4 230,906 - MMP_RS01225 0.25 -4.3 230,912 - MMP_RS01225 0.27 -3.3 230,912 - MMP_RS01225 0.27 -1.8 230,912 - MMP_RS01225 0.27 -3.7 230,955 - MMP_RS01225 0.42 -2.1 230,974 + MMP_RS01225 0.49 -4.7 230,977 - MMP_RS01225 0.50 -3.7 230,977 - MMP_RS01225 0.50 -0.3 231,095 - -1.2 231,162 - -1.0 231,357 - -0.2 231,734 + -0.7 232,022 + -1.5
Or see this region's nucleotide sequence