Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00845 and MMP_RS00850 are separated by 33 nucleotides MMP_RS00850 and MMP_RS00855 are separated by 69 nucleotides MMP_RS00855 and MMP_RS00860 are separated by 4 nucleotides
MMP_RS00845: MMP_RS00845 - acetate--CoA ligase, at 158,021 to 159,931
_RS00845
MMP_RS00850: MMP_RS00850 - TIGR01177 family methyltransferase, at 159,965 to 161,017
_RS00850
MMP_RS00855: MMP_RS00855 - DUF367 family protein, at 161,087 to 161,602
_RS00855
MMP_RS00860: MMP_RS00860 - 50S ribosomal protein L40e, at 161,607 to 161,750
_RS00860
Position (kb) 159
160
161
162 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 159.061 kb on - strand, within MMP_RS00845 at 159.099 kb on - strand, within MMP_RS00845 at 159.165 kb on + strand, within MMP_RS00845 at 159.172 kb on + strand, within MMP_RS00845 at 159.232 kb on - strand, within MMP_RS00845 at 159.233 kb on + strand, within MMP_RS00845 at 159.235 kb on - strand, within MMP_RS00845 at 159.235 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.238 kb on + strand, within MMP_RS00845 at 159.238 kb on + strand, within MMP_RS00845 at 159.669 kb on - strand, within MMP_RS00845 at 159.929 kb on - strand at 159.988 kb on - strand at 159.988 kb on - strand at 159.988 kb on - strand at 159.989 kb on + strand at 159.998 kb on + strand at 160.077 kb on + strand, within MMP_RS00850 at 160.119 kb on + strand, within MMP_RS00850 at 160.185 kb on + strand, within MMP_RS00850 at 160.344 kb on + strand, within MMP_RS00850 at 160.481 kb on - strand, within MMP_RS00850 at 160.542 kb on - strand, within MMP_RS00850 at 160.787 kb on - strand, within MMP_RS00850 at 161.052 kb on - strand at 161.052 kb on - strand at 161.052 kb on - strand at 161.059 kb on - strand at 161.060 kb on + strand at 161.067 kb on - strand at 161.067 kb on - strand at 161.067 kb on - strand at 161.068 kb on + strand at 161.068 kb on + strand at 161.069 kb on - strand at 161.069 kb on - strand at 161.069 kb on - strand at 161.070 kb on + strand at 161.070 kb on + strand at 161.071 kb on - strand at 161.071 kb on - strand at 161.071 kb on - strand at 161.072 kb on + strand at 161.072 kb on + strand at 161.100 kb on - strand at 161.101 kb on + strand at 161.102 kb on - strand at 161.102 kb on - strand at 161.134 kb on - strand at 161.134 kb on - strand at 161.176 kb on - strand, within MMP_RS00855 at 161.214 kb on - strand, within MMP_RS00855 at 161.260 kb on - strand, within MMP_RS00855 at 161.262 kb on - strand, within MMP_RS00855 at 161.408 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.749 kb on + strand at 161.867 kb on + strand at 161.885 kb on + strand at 161.916 kb on - strand at 161.981 kb on + strand at 162.004 kb on + strand at 162.007 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 159,061 - MMP_RS00845 0.54 -1.6 159,099 - MMP_RS00845 0.56 +0.3 159,165 + MMP_RS00845 0.60 +1.7 159,172 + MMP_RS00845 0.60 -4.4 159,232 - MMP_RS00845 0.63 +0.0 159,233 + MMP_RS00845 0.63 +0.1 159,235 - MMP_RS00845 0.64 +0.8 159,235 - MMP_RS00845 0.64 +0.3 159,237 - MMP_RS00845 0.64 -0.9 159,237 - MMP_RS00845 0.64 +0.3 159,237 - MMP_RS00845 0.64 +1.2 159,237 - MMP_RS00845 0.64 -1.3 159,238 + MMP_RS00845 0.64 -1.5 159,238 + MMP_RS00845 0.64 +0.3 159,669 - MMP_RS00845 0.86 +0.4 159,929 - -0.4 159,988 - +4.0 159,988 - +2.0 159,988 - -0.3 159,989 + +0.0 159,998 + +0.2 160,077 + MMP_RS00850 0.11 +0.9 160,119 + MMP_RS00850 0.15 -3.4 160,185 + MMP_RS00850 0.21 -3.6 160,344 + MMP_RS00850 0.36 -1.5 160,481 - MMP_RS00850 0.49 -2.7 160,542 - MMP_RS00850 0.55 -3.8 160,787 - MMP_RS00850 0.78 -5.1 161,052 - +0.8 161,052 - +0.8 161,052 - -0.5 161,059 - +0.5 161,060 + -2.2 161,067 - +1.7 161,067 - -2.1 161,067 - -1.6 161,068 + +2.1 161,068 + -1.5 161,069 - +0.6 161,069 - +0.5 161,069 - +0.1 161,070 + -3.5 161,070 + -0.6 161,071 - -0.3 161,071 - +0.9 161,071 - +0.8 161,072 + -0.4 161,072 + -0.1 161,100 - -1.9 161,101 + +0.9 161,102 - -0.1 161,102 - +1.9 161,134 - +1.0 161,134 - +0.6 161,176 - MMP_RS00855 0.17 +0.7 161,214 - MMP_RS00855 0.25 +1.4 161,260 - MMP_RS00855 0.34 +0.6 161,262 - MMP_RS00855 0.34 -0.5 161,408 + MMP_RS00855 0.62 -0.8 161,436 + MMP_RS00855 0.68 -0.9 161,436 + MMP_RS00855 0.68 +0.6 161,749 + +0.1 161,867 + -3.4 161,885 + -0.4 161,916 - +0.1 161,981 + -3.0 162,004 + +0.5 162,007 + +1.9
Or see this region's nucleotide sequence