Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08700

Experiment: McCas_formate_complete Chloride 250 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08690 and MMP_RS08695 are separated by 17 nucleotidesMMP_RS08695 and MMP_RS08700 are separated by 10 nucleotidesMMP_RS08700 and MMP_RS08705 are separated by 12 nucleotidesMMP_RS08705 and MMP_RS08710 are separated by 1 nucleotides MMP_RS08690: MMP_RS08690 - RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5, at 1,627,999 to 1,629,000 _RS08690 MMP_RS08695: MMP_RS08695 - ferredoxin family protein, at 1,629,018 to 1,629,224 _RS08695 MMP_RS08700: MMP_RS08700 - hypothetical protein, at 1,629,235 to 1,629,918 _RS08700 MMP_RS08705: MMP_RS08705 - sulfopyruvate decarboxylase subunit beta, at 1,629,931 to 1,630,488 _RS08705 MMP_RS08710: MMP_RS08710 - HisA/HisF family protein, at 1,630,490 to 1,631,158 _RS08710 Position (kb) 1629 1630Strain fitness (log2 ratio) -1 0 1 2 3 4at 1628.279 kb on + strand, within MMP_RS08690at 1628.456 kb on - strand, within MMP_RS08690at 1628.456 kb on - strand, within MMP_RS08690at 1628.457 kb on + strand, within MMP_RS08690at 1628.486 kb on + strand, within MMP_RS08690at 1628.489 kb on - strand, within MMP_RS08690at 1628.618 kb on + strand, within MMP_RS08690at 1628.619 kb on - strand, within MMP_RS08690at 1628.620 kb on + strand, within MMP_RS08690at 1628.622 kb on + strand, within MMP_RS08690at 1628.712 kb on + strand, within MMP_RS08690at 1628.872 kb on + strand, within MMP_RS08690at 1628.876 kb on - strand, within MMP_RS08690at 1628.918 kb on - strandat 1628.961 kb on - strandat 1628.988 kb on + strandat 1629.125 kb on + strandat 1629.442 kb on - strand, within MMP_RS08700at 1629.490 kb on - strand, within MMP_RS08700at 1629.491 kb on + strand, within MMP_RS08700at 1629.495 kb on + strand, within MMP_RS08700at 1629.532 kb on + strand, within MMP_RS08700at 1629.559 kb on - strand, within MMP_RS08700at 1629.561 kb on - strand, within MMP_RS08700at 1629.691 kb on - strand, within MMP_RS08700at 1629.691 kb on - strand, within MMP_RS08700at 1629.692 kb on + strand, within MMP_RS08700at 1629.701 kb on + strand, within MMP_RS08700at 1629.864 kb on + strandat 1629.917 kb on + strandat 1629.965 kb on + strandat 1629.979 kb on - strandat 1630.030 kb on - strand, within MMP_RS08705at 1630.031 kb on + strand, within MMP_RS08705at 1630.094 kb on + strand, within MMP_RS08705at 1630.143 kb on - strand, within MMP_RS08705at 1630.144 kb on + strand, within MMP_RS08705at 1630.283 kb on + strand, within MMP_RS08705at 1630.291 kb on - strand, within MMP_RS08705at 1630.419 kb on - strand, within MMP_RS08705at 1630.420 kb on + strand, within MMP_RS08705at 1630.488 kb on - strandat 1630.492 kb on + strandat 1630.521 kb on + strandat 1630.609 kb on - strandat 1630.610 kb on + strandat 1630.626 kb on - strandat 1630.626 kb on - strandat 1630.627 kb on + strandat 1630.627 kb on + strandat 1630.627 kb on + strandat 1630.652 kb on - strandat 1630.655 kb on + strandat 1630.661 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Chloride 250 mM
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1,628,279 + MMP_RS08690 0.28 +0.9
1,628,456 - MMP_RS08690 0.46 +0.4
1,628,456 - MMP_RS08690 0.46 -0.0
1,628,457 + MMP_RS08690 0.46 +0.5
1,628,486 + MMP_RS08690 0.49 +1.1
1,628,489 - MMP_RS08690 0.49 +0.4
1,628,618 + MMP_RS08690 0.62 -0.9
1,628,619 - MMP_RS08690 0.62 +0.1
1,628,620 + MMP_RS08690 0.62 -0.5
1,628,622 + MMP_RS08690 0.62 +0.1
1,628,712 + MMP_RS08690 0.71 +0.4
1,628,872 + MMP_RS08690 0.87 +0.2
1,628,876 - MMP_RS08690 0.88 -0.0
1,628,918 - -0.6
1,628,961 - -0.0
1,628,988 + -0.7
1,629,125 + +3.9
1,629,442 - MMP_RS08700 0.30 +0.7
1,629,490 - MMP_RS08700 0.37 -0.7
1,629,491 + MMP_RS08700 0.37 -0.3
1,629,495 + MMP_RS08700 0.38 -0.5
1,629,532 + MMP_RS08700 0.43 -0.4
1,629,559 - MMP_RS08700 0.47 -0.4
1,629,561 - MMP_RS08700 0.48 +0.3
1,629,691 - MMP_RS08700 0.67 -0.1
1,629,691 - MMP_RS08700 0.67 -0.4
1,629,692 + MMP_RS08700 0.67 +0.0
1,629,701 + MMP_RS08700 0.68 -0.2
1,629,864 + -0.0
1,629,917 + -0.4
1,629,965 + -1.2
1,629,979 - +0.3
1,630,030 - MMP_RS08705 0.18 +1.1
1,630,031 + MMP_RS08705 0.18 -0.4
1,630,094 + MMP_RS08705 0.29 +0.6
1,630,143 - MMP_RS08705 0.38 +0.2
1,630,144 + MMP_RS08705 0.38 +0.3
1,630,283 + MMP_RS08705 0.63 -1.0
1,630,291 - MMP_RS08705 0.65 -0.5
1,630,419 - MMP_RS08705 0.87 +0.8
1,630,420 + MMP_RS08705 0.88 -1.6
1,630,488 - -0.1
1,630,492 + -0.3
1,630,521 + -0.1
1,630,609 - -0.5
1,630,610 + -0.9
1,630,626 - -0.7
1,630,626 - -0.9
1,630,627 + -0.1
1,630,627 + -1.6
1,630,627 + -0.2
1,630,652 - -1.2
1,630,655 + -0.7
1,630,661 - +0.1

Or see this region's nucleotide sequence