Experiment: McCas_formate_complete Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01855 and MMP_RS01860 are separated by 225 nucleotides MMP_RS01860 and MMP_RS01865 are separated by 90 nucleotides MMP_RS01865 and MMP_RS01870 are separated by 126 nucleotides
MMP_RS01855: MMP_RS01855 - 2-hydroxyacyl-CoA dehydratase family protein, at 342,467 to 343,588
_RS01855
MMP_RS01860: MMP_RS01860 - hypothetical protein, at 343,814 to 344,128
_RS01860
MMP_RS01865: MMP_RS01865 - GPR1/FUN34/YaaH family transporter, at 344,219 to 344,812
_RS01865
MMP_RS01870: MMP_RS01870 - acyl-CoA dehydratase activase, at 344,939 to 345,679
_RS01870
Position (kb) 344
345 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 343.318 kb on + strand, within MMP_RS01855 at 343.403 kb on - strand, within MMP_RS01855 at 343.488 kb on + strand at 343.527 kb on + strand at 343.646 kb on - strand at 343.657 kb on - strand at 343.657 kb on - strand at 343.657 kb on - strand at 343.658 kb on + strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.669 kb on + strand at 343.669 kb on + strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.677 kb on + strand at 343.688 kb on + strand at 343.849 kb on + strand, within MMP_RS01860 at 343.849 kb on + strand, within MMP_RS01860 at 343.849 kb on + strand, within MMP_RS01860 at 343.849 kb on + strand, within MMP_RS01860 at 343.879 kb on - strand, within MMP_RS01860 at 343.879 kb on - strand, within MMP_RS01860 at 343.896 kb on + strand, within MMP_RS01860 at 343.911 kb on + strand, within MMP_RS01860 at 343.992 kb on + strand, within MMP_RS01860 at 344.004 kb on - strand, within MMP_RS01860 at 344.023 kb on - strand, within MMP_RS01860 at 344.096 kb on - strand, within MMP_RS01860 at 344.099 kb on + strand at 344.103 kb on + strand at 344.115 kb on + strand at 344.128 kb on - strand at 344.182 kb on - strand at 344.182 kb on - strand at 344.182 kb on - strand at 344.183 kb on + strand at 344.184 kb on - strand at 344.184 kb on - strand at 344.185 kb on + strand at 344.186 kb on - strand at 344.187 kb on + strand at 344.187 kb on + strand at 344.388 kb on + strand, within MMP_RS01865 at 344.790 kb on + strand at 344.819 kb on + strand at 344.856 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.859 kb on + strand at 344.859 kb on + strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.861 kb on + strand at 344.861 kb on + strand at 344.873 kb on + strand at 344.884 kb on - strand at 344.910 kb on - strand at 344.913 kb on + strand at 344.924 kb on + strand at 345.036 kb on + strand, within MMP_RS01870 at 345.078 kb on + strand, within MMP_RS01870 at 345.139 kb on + strand, within MMP_RS01870 at 345.140 kb on - strand, within MMP_RS01870 at 345.141 kb on + strand, within MMP_RS01870 at 345.289 kb on - strand, within MMP_RS01870 at 345.372 kb on + strand, within MMP_RS01870 at 345.372 kb on + strand, within MMP_RS01870 at 345.485 kb on - strand, within MMP_RS01870 at 345.560 kb on - strand, within MMP_RS01870 at 345.605 kb on + strand, within MMP_RS01870 at 345.614 kb on - strand at 345.671 kb on - strand at 345.729 kb on - strand at 345.735 kb on - strand at 345.739 kb on - strand at 345.739 kb on - strand at 345.740 kb on + strand at 345.743 kb on - strand at 345.756 kb on + strand at 345.757 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.775 kb on + strand at 345.775 kb on + strand at 345.783 kb on - strand at 345.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Chloride 250 mM remove 343,318 + MMP_RS01855 0.76 +0.0 343,403 - MMP_RS01855 0.83 -2.5 343,488 + +0.3 343,527 + +0.3 343,646 - -0.1 343,657 - -1.1 343,657 - -0.9 343,657 - -0.2 343,658 + +0.3 343,666 - +0.5 343,666 - -1.2 343,666 - -1.0 343,666 - +0.5 343,666 - -1.2 343,666 - +0.5 343,666 - +0.4 343,666 - -0.7 343,667 + -0.8 343,667 + +1.6 343,667 + +0.8 343,667 + +0.2 343,667 + +0.4 343,667 + -0.6 343,667 + +1.0 343,667 + +0.1 343,669 + -0.8 343,669 + +1.1 343,676 - +1.7 343,676 - +0.1 343,676 - +0.5 343,676 - +0.3 343,676 - +4.4 343,676 - +0.6 343,677 + -1.3 343,688 + -0.4 343,849 + MMP_RS01860 0.11 -1.0 343,849 + MMP_RS01860 0.11 -4.0 343,849 + MMP_RS01860 0.11 +0.4 343,849 + MMP_RS01860 0.11 -0.1 343,879 - MMP_RS01860 0.21 -0.7 343,879 - MMP_RS01860 0.21 +1.1 343,896 + MMP_RS01860 0.26 -0.9 343,911 + MMP_RS01860 0.31 -0.7 343,992 + MMP_RS01860 0.57 -1.1 344,004 - MMP_RS01860 0.60 -0.7 344,023 - MMP_RS01860 0.66 -0.6 344,096 - MMP_RS01860 0.90 -0.2 344,099 + +0.7 344,103 + -0.8 344,115 + +0.3 344,128 - +0.5 344,182 - -0.6 344,182 - +0.6 344,182 - -0.1 344,183 + -3.2 344,184 - +0.5 344,184 - -0.3 344,185 + -1.1 344,186 - +0.4 344,187 + +0.7 344,187 + +0.6 344,388 + MMP_RS01865 0.28 -0.3 344,790 + -0.3 344,819 + +1.0 344,856 - +0.4 344,858 - +1.1 344,858 - -0.2 344,858 - +0.5 344,858 - +0.3 344,859 + -0.6 344,859 + +0.3 344,860 - +1.0 344,860 - +0.1 344,860 - +1.6 344,860 - +0.3 344,861 + +0.6 344,861 + -0.6 344,873 + +0.0 344,884 - -0.7 344,910 - -0.3 344,913 + -0.6 344,924 + +0.4 345,036 + MMP_RS01870 0.13 -0.3 345,078 + MMP_RS01870 0.19 -0.1 345,139 + MMP_RS01870 0.27 -0.8 345,140 - MMP_RS01870 0.27 +0.4 345,141 + MMP_RS01870 0.27 -1.0 345,289 - MMP_RS01870 0.47 -0.8 345,372 + MMP_RS01870 0.58 -0.4 345,372 + MMP_RS01870 0.58 +0.3 345,485 - MMP_RS01870 0.74 -0.0 345,560 - MMP_RS01870 0.84 +0.3 345,605 + MMP_RS01870 0.90 -0.4 345,614 - +0.7 345,671 - -0.1 345,729 - -0.6 345,735 - +0.0 345,739 - +0.3 345,739 - -0.0 345,740 + -0.1 345,743 - -1.1 345,756 + -1.7 345,757 - +0.6 345,774 - -2.9 345,774 - -0.2 345,774 - +0.4 345,775 + +4.3 345,775 + -1.0 345,783 - -3.4 345,789 + -1.5
Or see this region's nucleotide sequence