Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01680

Experiment: McCas_formate_complete Chloride 250 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01670 and MMP_RS01675 are separated by 8 nucleotidesMMP_RS01675 and MMP_RS01680 are separated by 314 nucleotidesMMP_RS01680 and MMP_RS01685 overlap by 8 nucleotidesMMP_RS01685 and MMP_RS01690 overlap by 4 nucleotides MMP_RS01670: MMP_RS01670 - methanogenesis marker 5 protein, at 307,706 to 308,146 _RS01670 MMP_RS01675: MMP_RS01675 - hypothetical protein, at 308,155 to 308,610 _RS01675 MMP_RS01680: MMP_RS01680 - phenylacetate--CoA ligase, at 308,925 to 310,169 _RS01680 MMP_RS01685: MMP_RS01685 - indolepyruvate oxidoreductase subunit beta, at 310,162 to 310,761 _RS01685 MMP_RS01690: MMP_RS01690 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 310,758 to 312,596 _RS01690 Position (kb) 308 309 310 311Strain fitness (log2 ratio) -1 0 1at 308.172 kb on - strandat 308.340 kb on + strand, within MMP_RS01675at 308.592 kb on + strandat 308.592 kb on + strandat 308.595 kb on + strandat 308.612 kb on - strandat 308.613 kb on + strandat 308.613 kb on + strandat 308.614 kb on - strandat 308.615 kb on + strandat 308.671 kb on - strandat 308.671 kb on - strandat 308.672 kb on + strandat 308.674 kb on + strandat 308.676 kb on + strandat 308.677 kb on - strandat 308.690 kb on - strandat 308.708 kb on - strandat 308.717 kb on + strandat 308.725 kb on - strandat 308.726 kb on + strandat 308.726 kb on + strandat 308.727 kb on - strandat 308.854 kb on - strandat 309.019 kb on - strandat 309.020 kb on + strandat 309.036 kb on - strandat 309.092 kb on - strand, within MMP_RS01680at 309.097 kb on + strand, within MMP_RS01680at 309.110 kb on - strand, within MMP_RS01680at 309.276 kb on + strand, within MMP_RS01680at 309.344 kb on - strand, within MMP_RS01680at 309.489 kb on - strand, within MMP_RS01680at 309.489 kb on - strand, within MMP_RS01680at 309.489 kb on - strand, within MMP_RS01680at 309.490 kb on + strand, within MMP_RS01680at 309.509 kb on - strand, within MMP_RS01680at 309.821 kb on - strand, within MMP_RS01680at 309.828 kb on + strand, within MMP_RS01680at 309.879 kb on + strand, within MMP_RS01680at 309.882 kb on - strand, within MMP_RS01680at 309.883 kb on + strand, within MMP_RS01680at 309.898 kb on - strand, within MMP_RS01680at 309.903 kb on - strand, within MMP_RS01680at 309.943 kb on + strand, within MMP_RS01680at 310.049 kb on - strandat 310.050 kb on + strandat 310.065 kb on - strandat 310.065 kb on - strandat 310.065 kb on - strandat 310.397 kb on - strand, within MMP_RS01685at 310.486 kb on - strand, within MMP_RS01685at 310.745 kb on + strandat 310.997 kb on + strand, within MMP_RS01690at 311.111 kb on - strand, within MMP_RS01690

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Chloride 250 mM
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308,172 - -0.4
308,340 + MMP_RS01675 0.41 -0.4
308,592 + +1.0
308,592 + +0.3
308,595 + -0.6
308,612 - -0.1
308,613 + -0.4
308,613 + -0.0
308,614 - +0.1
308,615 + -0.1
308,671 - -0.5
308,671 - -0.3
308,672 + -1.4
308,674 + -0.5
308,676 + -0.9
308,677 - -0.3
308,690 - -0.3
308,708 - -0.1
308,717 + +0.2
308,725 - +0.4
308,726 + -0.6
308,726 + -1.3
308,727 - -0.5
308,854 - +0.2
309,019 - -0.5
309,020 + -1.1
309,036 - -0.3
309,092 - MMP_RS01680 0.13 -1.3
309,097 + MMP_RS01680 0.14 +0.2
309,110 - MMP_RS01680 0.15 +0.1
309,276 + MMP_RS01680 0.28 -0.6
309,344 - MMP_RS01680 0.34 -0.5
309,489 - MMP_RS01680 0.45 -0.3
309,489 - MMP_RS01680 0.45 +0.0
309,489 - MMP_RS01680 0.45 +0.2
309,490 + MMP_RS01680 0.45 +0.1
309,509 - MMP_RS01680 0.47 +0.8
309,821 - MMP_RS01680 0.72 +0.4
309,828 + MMP_RS01680 0.73 +0.1
309,879 + MMP_RS01680 0.77 -0.4
309,882 - MMP_RS01680 0.77 -1.5
309,883 + MMP_RS01680 0.77 -0.2
309,898 - MMP_RS01680 0.78 -0.9
309,903 - MMP_RS01680 0.79 -0.2
309,943 + MMP_RS01680 0.82 +0.1
310,049 - -0.6
310,050 + -1.1
310,065 - -0.1
310,065 - -0.4
310,065 - -0.1
310,397 - MMP_RS01685 0.39 +0.2
310,486 - MMP_RS01685 0.54 -0.3
310,745 + +0.5
310,997 + MMP_RS01690 0.13 -0.6
311,111 - MMP_RS01690 0.19 +0.5

Or see this region's nucleotide sequence